最新刊期
      卷 0 ， 期 0 ， 2020
      • TANG Ganghua
        Issue 11, (2000)
        摘要：After oral administration of perlolyrine and [2-15N] perlolyrine, urines of rats were hydrolyzed with glucuronidase, basified with NaHCO3-Na2CO3, and extracted with ethyl ether-iso-propyl alcohol etc. The organic phases (neutral and basic fractions) were concentrated for TMS derivatives. The aqueous phase were acidified with sulfuric acid, taken to dryness and extracted with methanol (water soluble acidic fractions) and concentrated for TMS derivatives. The TMS derivatives were determined by GC-MS. Perlolyrine and one metabolite were found from the neutral and basic fractions, and two different metabolites were found from the water soluble acidic fractions. It is proposed that the major metabolic pathways of perlolyrine are the hydroxylation of perlolyrine and the oxidition of its hydroxylmethyl group.  
        关键词：Perlolyrine;[2-15N] perlolyrine;Metabolism;GC-MS   
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        发布时间：2021-03-12
      • LI Qingnuan,ZHANG Xiaodong,SHENG Rong,MING Xiaofeng XU Jianying,HU Weiqing,LI Wenxin
        Issue 11, (2000)
        摘要：Carrier-free 188Re- and carrier-added 188Re-AEDP (1-amino-ethylene diphosphonic acid) were synthesized by using 188Re tracer eluted from 188W/188Re generator. The distributions of both 188Re-labelled compounds in rats were measured and the dependence of their biodistribution on the rhenium carrier was studied. The results showed much higher skeletal uptake, T/NT and more rapid blood clearance when using carrier-added 188Re. It was found that carrier has effect on the skeletal uptake of 188Re-AEDP. Thus 188Re-AEDP is a potential radiopharmaceutical which may be therapeutically beneficial to bone metastases.  
        关键词：Re-AEDP;Carrier;Biodistribution@   
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        发布时间：2021-03-12
      • DING Zigeng,YU Junfeng,YIN Duanzhi,HU Weiqing,WANG Yongxian
        Issue 11, (2000)
        摘要：SnCl2 reduction solution together with sodium gluconate was chosen to reduce Na188ReO4- and label human IgG. The labelling conditions were optimized. It was found that the labelling efficiency could reach 90% under the following condition: 1g/L protein with 5000 mole ratio AA in 2h reducing time at pH= 3, in mingled with 2.6×10-2- 8.0× 10-2mol/L SnCl2 in sodium gluconate and 188ReO4- at ambient temperature. The factors effecting labelling efficiency were studied and discussed.  
        关键词：Labelling efficiency;188Re;Human immunoglobulin   
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        发布时间：2021-03-12
      • YIN Duanzhi,HU Weiqing,SHI Xichang,CAO Benhong ZHOU Wei,YU Junfeng,WANG Yongxian,KNAPP F F
        Issue 11, (2000)
        摘要：A clinical-scale 188W-188Re generator loaded with more than one curie of 188W was successfully prepared. The yield of 188Re from generator eluted with 10mL isotonic saline solution was 90%. The breakthrough of 188W was 4 × 10-7. The purity of eluted 188Re was satisfactory. Radionuclide purity was >99.9%. Radiochemical purity was >99% of the 188Re as perrhenate ion. Concentration of aluminium in eluate was ～ 1 μ g Al/mL. The generator provides a convenient supply of sterile 188Re as sodium perrhenate in injectable saline solution ready for use in the preparation of 188Re labelled therapeutic reagents.  
        关键词：W-188Re;Generator;Therapy;Radionuclide   
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        发布时间：2021-03-12
      • ZHANG Rongjun,WAN Weixing,ZHOU Yan,TAN Chen,TAO Yonghui WANG Bochen,HAN Yumin,JIN Jian
        Issue 11, (2000)
        摘要：Comparsion of two labeling methods showed that 99mTc-recombinant hirudin (99mTc-rH) prepared by modified 2-imiothiolane method had higher radiochemical purity and stability than that by direct labeling method. Biodistribution of 99mTc-rH in mice showed that the radioactivity in heart, lung, liver and spleen was very low, while that in kidney was high. 99mTc-rH was cleared rapidly in blood and excreted mostly from kidney. The arterial and venous thrombi in rabbits were clearly imaged 60min after injection of 99mTc-rH, T/B ratio was 2.24 and 1.95 respectively. The rH would be a potential agent for diagnosis of thrombotic disease.  
        关键词：Hirudin;99mTc;Thrombosis;Radionuclide imaging;Thrombin   
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        发布时间：2021-03-12
      • ZHOU Guangming,LI Wenjian,WANG Jufang,HE Jing,GAO Qingxiang,CHEN Wei,WEI Zengquan
        Issue 11, (2000)
        摘要：In order to study the mechanism of radiobiological effects, the inverse pulsed-field gel electrophoresis has been applied to separate DNA from hepatoma SMMC-7721 cells cultured for various hours after exposed to γ -ray. Results showed that, in the samples repaired for 0 and 24 hours, the percentages of DNA released from plug (PR) increased with dose. The fragments were mainly 1-2Mbp and the yields of DSB in the two kinds of samples were 0.38DSBs/100Mbp· Gy and 0.06DSBs/100Mbp·Gy, respectively. Thus 84% DSBs were rejoined. In the samples repaired for 48 hours, the remaining DSB fragments induced with different doses were similar to the control and seem to have nothing to do with dose. It is elucidated that DSBs induced by γ -ray are easy to be rejoined and the remaining DSBs are the cause of cell death.  
        关键词：γ -ray;DNA double-strand break;Electrophoresis;Hepatoma ce?   
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        发布时间：2021-03-12
      • ZHANG Rongjun,LIANG Gaolin,WAN Weixing,CAI Gangming,YIE Wanzhong TAO Yonghui,ZHANG Lianfeng,TAN Cheng,JIN Jian
        Issue 11, (2000)
        摘要：Human serum albumin (HSA) was purified by FPLC with HiTrap Q column. Galactosyl neoglycoalbumin (NGA) was synthesized by the covalent coupling of a bifunctional reagent, 2-imino-2-ethyloxymethyl- 1-thiogalactose, to HSA. The average number of galactose groups was 30 in each NGA. NGA was labeled directly with Na99mTcO4. The radiochemical purity of 99mTc-NGA was over 90%. Biodistribution in mice, liver imaging in rat, rabbit and dog all show that 99mTc-NGA is a good hepatic receptor imaging agent.  
        关键词：NGA;99mTc;Hepatic receptor imaging agent;Bifunctional reagent;Glycoprotein?   
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        发布时间：2021-03-12
      • LU Gongxu,ZHANG Yadong,WANG Xuebin
        Issue 11, (2000)
        摘要：A new tridentate N-(2-mercapto-2-methylpropyl)- N,N-diethylethylene diamine (MDEDA) containing the SNN donor was synthesized and characterized by IR and 1H NMR. The tridentate and various monodentate thiols using 99mTc(V)-gluconate as precusor, chelates to the TcO3+ core. Thus four "3 + 1," mixed ligand complexes of general formula [TcO(Et2NCH2CH2 NCHC(CH3)2S] [SR] were prepared. The labeling efficiency were all higher than 90%. The effects of pH value, temperature and reaction time were studied. The biodistribution results in mice and rabbits indicated that the uptake and retention of these complexes in blood was much higher and longer than those in other organs. The retention of complex 5c can be retend in blood was longer than the retention of others. Perhaps it can satisfy the requirement of blood-pool imaging agent.  
        关键词：mTc mixed-ligand complexes;Biodistribution;Retention in blood   
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        发布时间：2021-03-12
      • WU Jing,MIAO Weibing,YE Defu,LIN Haoxue
        Issue 11, (2000)
        摘要：This paper reported 20 cases of joint imaging in active Rheumatoid Arthritis (RA) by using 99mTc labeled human immunoglobulin G(HIG), 16 of which were compared with 99mTc-MDP. Abnormal images were found in 99mTc-HIG imaging of all patients, showing increased uptake in inflammatory synovial membranes of joint capsules. The concordance of 99mTc-HIG imaging with clinical findings was much better than that of 99mTc-MAP bone imaging. Significant correlation was found between clinical score with image score, as well as uptake score of radionuclide with clinical symptoms (including pain or swelling) of the joints involved (P<0.01). It was concluded that 99mTc-HIG joint imaging was a simple, safe, noninvasive and objective method for early detection of RA. It can also demonstrate synovitis and assess of lammatory activity in involved joint. By the way the diagnostic value of 99mTc-HIG seems better than 99mTc- MDP bone imaging.  
        关键词：Rheumatoid Arthritis;99mTc labeled human immunoglobulin;Joint imaging   
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        发布时间：2021-03-12
      • YU Yongli,ZHU Ruisen JI Hong,LUO Quanyong
        Issue 11, (2000)
        摘要：To investigate clinical value of 14C-urea breath test (14C-UBT) for the diagnosis of Helicobacter pylori(Hp), 70 patients were both pertormed gastroscopy (taking gastric mucosae biopsy for rapid urease test and histology) and14C-UBT (some patients by Hp-IgG or DNAHp test also) within two days. The positive cases of both rapid urease test and histology was defined as a gold standard" of Hp-positive, whereas the negative cases of both rapid urease test and histology as "gold standard" of Hp-negative. The sensitivity of 14C-UBT was 93.2%, the specificity 73.1%, and the diagnostic accuracy 85.7%. The difference (comparing with " gold standard") was not marked (x2 = 0.9 < x20.05(1) = 3.84, P> 0.05). But the diagnostic accuracy of 14C-UBT (85.7%) and Hp-IgG (50%) had a marked difference (x2= 13.80>x2 0.01(1)=6.64, P<0.01). Therefore, the 14C-UBT was easy to operate, reliable and suitable for clinical application.  
        关键词：Helicobacter pylori;14C-urea breath test   
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        发布时间：2021-03-12
      • JIN Xiumu,XIE Wenhui,YU Zhichang,ZHANG Peiling
        Issue 11, (2000)
        摘要：Three tumor markers or CEA, CYFRA21-2 and NSE were assayed in 239 cases with lung cancer (adenocarcinoma 129, squamous-cell carcinoma 59, small cell lung cancer 35, mixed type of adenocarcinoma and squamous-cell carcinoma 16) and 66 cases with benign lung disease. The positive rate of CEA, CYFRA21-1 and NSE for detecting lung cancer were 51.4%, 43.5% and 48.1% respectively. It seemed there was a relationship between the sensitivity and the pathologic patterns of lung cancer. The highest diagnostic sensitivities were 76.3% for CYFRA21-1 in the detection of squamous-cell carcinoma, 57.4% for CEA in adenocarcinoma and 94.3% for NSE in the small cell lung cancer respectively. In 49 cases with pleural effusion (adenocarcinoma 27, small cell lung cancer 7, benign disease 15), three tumor markers in serum and pleural fluid were both measued. The results indicated that the sensitivity of the CEA and CYFRA21-1 in pleural fluid in patients /with adenocarcinoma was superior to serum. The detection of the NSE in pleural fluid was a very reliable method in diagnosing the small cell lung cancer. The sensitivity and specificity of CEA, CYFRA21-1 and NSE in different pathologic patterns of lung cancer was compared and also the false positive and false negative in benign lung disease. Moreover, the clinical role and necessity of combined determination were also discussed.  
        关键词：CYFRA21-1;CEA;NSE;Lung cancer;Determinati?   
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        发布时间：2021-03-12
      • DIAO Yao,XING Fuqin
        Issue 11, (2000)
        摘要：Distribution of 99mTc-MIBI was studied in frozen section of rat thyroid tissue using autoradiography and γ counter detector at different times after cauda vein injection. The results demonstrated that the ability of thyroid to uptake 99mTc-MIBI was strongest at 3 minute after injection and the speed of excretion was slow in the period from 15 to 60 minutes and thus formed the retention period with the highest concentration. 99mTc-MIBI was rapidly eliminated after 60 minutes and autoradiographic film exhibited that large amount of 99mTc-MIBI distributed in follicular epithelial cells especilly in basal part.  
        关键词：mTc-MIBI;Thyroid;Autoradiogra?   
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        发布时间：2021-03-12
      • SHI Wei,ZHANG Yingjian,JIANG Changying
        Issue 11, (2000)
        摘要：The aim of this study was to evaluate the value of 99mTc-MIBI imaging in 46 cases of thyroid masses (18 malignant, 28 benign and 24 cases of postoperative relapse or metastatic thyroid cancer. Results showed that radioactivity filling in primary cancer lesion was observed in 14 out of 18 thyroid carcinoma, whereas only 9 out of 28 in benign lesions, 99mTc-MIBI imaging was positive in 19 out of 24 patients with postoperative relapse or lymph nods metastases. Its positivity was signicantly higher than, 131I imaging, thereby 99mTc-MIBI imaging was highly valuable in detecting early thyroid carcinoma, especially, in finding cot distant metastases and postoperative relapse.  
        关键词：Thyroid neoplasms;Radionuclide imaging;99mTc-M?   
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        发布时间：2021-03-12
      • CAO Guoxiang,ZHANG Yongxue
        Issue 11, (2000)
        摘要：To investigate the regularity of renal dynamic imaging in the various courses of diabetes mellitus, and then to provide the important evidence variety for diagnosis and treatment. The data of renal dynamic imaging was analyzed on 276 kidneys of 40 normal and 98 diabetic patients, and according to course of disease, the diabetic patients was divided into three groups: ≤1 year, 1-5 year and ≥5 year respectively. Based upon was grouped 99mTc-DTPA renal dynamic imaging and others, the renal functions into normal, approximately normal, Ⅰ, Ⅱand Ⅲ injury respectively, and the statistics and analysis of these data were caned out. The extent of renal function injury was related to the course of diseace significantly, and it showed that the longer the course, the heavier the damage of renal function, and there was a significant difference between them (P<0.01). There was radioactive retention in pelvis and/or calyxes in 23.5% patients together with" a stepwise curve" on renogram. 99mTc-DTPA renal dynamic imaging can effectively evaluate the extent of renal functions injury and was also valuable for the clinical. Also, the study indicated that in diabetic patients susceptible to urinary tract infection could aggravate the injury of renal function.  
        关键词：Diabetes mellitus;Renal dynamic imaging;99mTc-DH   
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        发布时间：2021-03-12
      • LIU Ning,JIN Jiannan,ZHANG Shuyuan,YANG Yuanyou
        Issue 11, (2000)
        摘要：I labeling of three human serum albumins(HSAs) produced by different methods were performed by Chloramine-T, with yields of more than 80%. 125I labelled HSAs were purified by Sephadex G50 column with elution of 0.05mol/L PBS(pH7.4) and analysed by HPLC on a Sepheragel TSK phenyl SPW Column (7.5mm × 75mm)with gradient elution. The metabolic process of 125I-labelled HSAs in rabbits was investigated and their biological half lifes was calculated. The result showed that no significant difference were found among the biological half lifes of the three kinds of HSAs in rabbits.  
        关键词：Human serum albumin;125I;Chloramine-T;HPLC;Biological half li?   
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        发布时间：2021-03-12
      • YAN Jian,CAI Jin,LU Zhe
        Issue 11, (2000)
        摘要：To observe the influence of nifidipine(NIF) in 99mTc-MIBI myocardial tomography imaging. 35 patients with coronary artery disease were divided into NIF group and nitroglycerine(NTC) group. 48 hours after rest imaging, each patient was administrated NIF or NTC sublingually, then myocardial imaging was performed at 1 and 5 hours after injection of 99mTc-MIBI, these imagings were compared and analyzed. In NIF group, 40.0% segments of low intake in rest imaging had improvement. But 33 segments at 1 hour imaging had lower intake than rest imaging, and 29 segments returned to normal at 5 hour. In NTC group, 69.5% segments of low intake in rest imaging had improvement. It was not seen at 1 hour that segments had lower intake than rest imaging. 90mTc-MIBI NIF myocardial tomography imaging revealed some myocardial segments had transient low intake at 1 hour imaging, the exact explaination requires more studies.  
        关键词：Nifedipine;Nitroglycerine;Radionuclide imaging;Nitril?   
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        发布时间：2021-03-12
      • JIA Wenbao,YAO Zeen,SU Tongling,WANG Xuezhi,YANG Huazhong
        Issue 11, (2000)
        摘要：According to the design need of the shielding of therapy head in the 14MeV neutron therapy system of Lanzhou University, this paper simulated the transfer process of neutron at a source to body surface distance of 100cm by Monte Carlo method (mcnp3b). The dose transmissivity of fast neutron on the complex shielding of various combination was calculated. Optimum scheme of shielding was designed. It supplied a scientific basis for the design of therapy head.  
        关键词：Monte Carlo method;Combined shield;Dose transmissivit   
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        发布时间：2021-03-12
      • LI Wenxin,ZHANG Xiaodong,LI Qinnuan
        Issue 11, (2000)
        摘要：The radionuclides currently used for cancer therapy are reviewed. The radionuclides involved are the pure β emitters, the β emitters with accompanying γ-ray emission suitable for imaging as well as the internal conversion electron and Auger electron emitters. The problems related to the production of these radionuclides in nuclear reactors are discussed. It is indicated that the spallation neutron source based on high intensity proton accelerator will be an optimum approach to the production of the radionuclides for cancer therapy.  
        关键词：Radionuclides;Cancer therapy;Nuclear reactor;Spallation neutron source   
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        发布时间：2021-03-12
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