A Preliminary study on the labelling of immunoglobulin with <sup>188</sup>Re

DING Zigeng; YU Junfeng; YIN Duanzhi; HU Weiqing; WANG Yongxian

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A Preliminary study on the labelling of immunoglobulin with ¹⁸⁸Re

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- A Preliminary study on the labelling of immunoglobulin with ¹⁸⁸Re
- NUCLEAR TECHNIQUES Issue 11, (2000)
- 作者机构：
  
  1. 中国科学院上海原子核研究所,上海,201800
  2. 中国科学院上海细胞生物研究所,上海,200031
  3. 中国科学院上海细胞生物研
- 作者简介：
- 基金信息：
- DOI：
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- Published：01 November 2000
- 稿件说明：
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[1]丁自更,于俊峰,尹端沚,胡伟清,汪勇先,王根凤,张茹萍,来文,谢弘.人血清IgG的~(188)Re标记条件初探[J].核技术,2000(11):762-766.

DING Zigeng, YU Junfeng, YIN Duanzhi, et al. A Preliminary study on the labelling of immunoglobulin with ¹⁸⁸Re[J]. Nuclear techniques, 2000, (11): 762-766.
[1]丁自更,于俊峰,尹端沚,胡伟清,汪勇先,王根凤,张茹萍,来文,谢弘.人血清IgG的~(188)Re标记条件初探[J].核技术,2000(11):762-766. DOI：

DING Zigeng, YU Junfeng, YIN Duanzhi, et al. A Preliminary study on the labelling of immunoglobulin with ¹⁸⁸Re[J]. Nuclear techniques, 2000, (11): 762-766. DOI：

摘要

以含葡萄糖酸钠配体的ＳｎＣｌ２为还原液，还原Ｎａ１８８ＲｅＯ４后，标记人血清ＩｇＧ，在ｐＨ＝３的条件下，１ｇ／Ｌ的蛋白质用高于５０００倍摩尔过量的抗坏血酸（ＡＡ）还原２ｈ后，与２．６×１０－２－８．０×１０－２ｍｏｌ／Ｌ的ＳｎＣｌ２－葡萄糖酸钠溶液及１８８－ＲｅＯ４－反应３ｈ，可以得到９０％的标记率，在该标记条件下，对影响标记率的因素作了初步的研究和探讨。

Abstract

SnCl2 reduction solution together with sodium gluconate was chosen to reduce Na188ReO4- and label human IgG. The labelling conditions were optimized. It was found that the labelling efficiency could reach 90% under the following condition: 1g/L protein with 5000 mole ratio AA in 2h reducing time at pH= 3

in mingled with 2.6×10-2- 8.0× 10-2mol/L SnCl2 in sodium gluconate and 188ReO4- at ambient temperature. The factors effecting labelling efficiency were studied and discussed.

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Shanghai Institute of Applied Physics, China Academy of Sciences, Shanghai 201800, China) 2

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A Preliminary study on the labelling of immunoglobulin with 188Re

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摘要

Abstract

关键词

Keywords

references

A Preliminary study on the labelling of immunoglobulin with ¹⁸⁸Re