Study of urinary metabolites of perlolyrine in rats by stable isotope tracing method

TANG Ganghua

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Study of urinary metabolites of perlolyrine in rats by stable isotope tracing method

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- Study of urinary metabolites of perlolyrine in rats by stable isotope tracing method
- NUCLEAR TECHNIQUES Issue 11, (2000)
- 作者机构：
  
  1. 第一军医大学南方医院,广州,510515
  2. 中国医学科学院-中国协和医科大学北京协和医院,北京,100730
- 作者简介：
- 基金信息：
- DOI：
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- Published：01 November 2000
- 稿件说明：
移动端阅览
[1]唐刚华,姜国辉,王世真,郑连芳.稳定同位素法研究川芎哚体内代谢[J].核技术,2000(11):753-756.

TANG Ganghua. Study of urinary metabolites of perlolyrine in rats by stable isotope tracing method[J]. Nuclear techniques, 2000, (11): 753-756.
[1]唐刚华,姜国辉,王世真,郑连芳.稳定同位素法研究川芎哚体内代谢[J].核技术,2000(11):753-756. DOI：

TANG Ganghua. Study of urinary metabolites of perlolyrine in rats by stable isotope tracing method[J]. Nuclear techniques, 2000, (11): 753-756. DOI：

摘要

分别灌胃给予川芎哚和［２－１５Ｎ］川哚后，尿样经酶水解、碱化、有机溶剂提取。有机相（碱性和中性部分）经浓缩并硅烷化；水相经酸化、吹干提取（提取物为酸性水溶部分）并硅烷化。用ＧＣ－ＭＳ测定。在碱性和中性提取物中，检出原型川穹哚及其一种代谢物；在酸性水溶性提取物中，检出川芎哚的另两种代谢物。结果表明，川芎哚体内代谢途径可能是川芎哚羟基化和川芎哚羟甲基的氧化。

Abstract

After oral administration of perlolyrine and [2-15N] perlolyrine

urines of rats were hydrolyzed with glucuronidase

basified with NaHCO3-Na2CO3

and extracted with ethyl ether-iso-propyl alcohol etc. The organic phases (neutral and basic fractions) were concentrated for TMS derivatives. The aqueous phase were acidified with sulfuric acid

taken to dryness and extracted with methanol (water soluble acidic fractions) and concentrated for TMS derivatives. The TMS derivatives were determined by GC-MS. Perlolyrine and one metabolite were found from the neutral and basic fractions

and two different metabolites were found from the water soluble acidic fractions. It is proposed that the major metabolic pathways of perlolyrine are the hydroxylation of perlolyrine and the oxidition of its hydroxylmethyl group.

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