<sup>125</sup>I labeling of human serum albumins and their metabolic process in rabbits

LIU Ning; JIN Jiannan; ZHANG Shuyuan; YANG Yuanyou

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¹²⁵I labeling of human serum albumins and their metabolic process in rabbits

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- ¹²⁵I labeling of human serum albumins and their metabolic process in rabbits
- NUCLEAR TECHNIQUES Issue 11, (2000)
- 作者机构：
  
  1. 四川大学原子核科学技术研究所
  2. 教育部辐射物理及技术重点实验室,成都,610064
  3. 教育部辐射物
- 作者简介：
- 基金信息：
- DOI：
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- Published：01 November 2000
- 稿件说明：
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[1]刘宁,金建南,张叔渊,杨远友.~(125)I标记人血白蛋白及其在家兔体内代谢的研究[J].核技术,2000(11):816-820.

LIU Ning, JIN Jiannan, ZHANG Shuyuan, et al. ¹²⁵I labeling of human serum albumins and their metabolic process in rabbits[J]. Nuclear techniques, 2000, (11): 816-820.
[1]刘宁,金建南,张叔渊,杨远友.~(125)I标记人血白蛋白及其在家兔体内代谢的研究[J].核技术,2000(11):816-820. DOI：

LIU Ning, JIN Jiannan, ZHANG Shuyuan, et al. ¹²⁵I labeling of human serum albumins and their metabolic process in rabbits[J]. Nuclear techniques, 2000, (11): 816-820. DOI：

摘要

采用氯胺－Ｔ法标记了３种不同来源的人血白蛋白。用预饱和的ＳｅｐｈａｄｅｘＧ５０柱分离纯化１２５Ｉ标记人血白蛋白，并用ＨＰＬＣ法对标记物进行分析鉴定。取分离后的１２５Ｉ标记白蛋白配制示踪液，进行家兔体内代谢试验。用氯胺－Ｔ方法，１２５Ｉ标记人血白蛋白的标记率＞８０％。代谢研究表明，成都生物制品研究所柱层析、低温乙醇工艺和加拿大技层析工艺生产的人血白蛋白，其生物半减期分别为９６．１１±０．０１、８５．７５±２．６２和９０．００±１．６９，三者之间无显著性差异（＞０．０５）。

Abstract

I labeling of three human serum albumins(HSAs) produced by different methods were performed by Chloramine-T

with yields of more than 80%. 125I labelled HSAs were purified by Sephadex G50 column with elution of 0.05mol/L PBS(pH7.4) and analysed by HPLC on a Sepheragel TSK phenyl SPW Column (7.5mm × 75mm)with gradient elution. The metabolic process of 125I-labelled HSAs in rabbits was investigated and their biological half lifes was calculated. The result showed that no significant difference were found among the biological half lifes of the three kinds of HSAs in rabbits.

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125I labeling of human serum albumins and their metabolic process in rabbits

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摘要

Abstract

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references

¹²⁵I labeling of human serum albumins and their metabolic process in rabbits