最新刊期
      卷 0 ， 期 0 ， 2020
      • Issue 11, (1998)
        摘要：To study the possibility of radioactive labelled vasoactive intestinal peptiede （VIP） for tumor VIP receptor imaging. 125I-VIP was prepared by chloramine-T method, and purified by sephadex G-50 column chromatography. The bioactivity and stability of 125I-VIP were measured by silica 60 F254 TLC and competition test to SGC7901 cell in vitro. The biodistribution of 125I-VIP was studied in the nude mice bearing tumor. The results showed that labelled rate of 125I was 73.8%, the specific activity was 18.2PBq/mol, the radiochemical purity（RCP） was over 98% and remained 96.3% after 48 days stored at m80oC. The specific binding of 125I-VIP to the SGC7901 cell was inhibited by VIP in dose dependence in the competition experiment. The radioactivity of tumor was higher than that of muscles in all phases （P<0.05-0.01）,the peak activity of tumor occured at 30min （3.58 ± 0.48ID%/g） and the peak ratio of T/N occured at 60min after the injection. The activity of lungs was obviously higher than that of blood, the intestine was always in low level. Most of the activity in the body was mainly eliminated from kidney. The present study demonstrated that the radioactive labelled VIP is a promosing agent for tumor VIP receptor scintigraphy.  
        关键词：VIP;Pharmacological distribution;Tumor;Gastric tumor;Animal;Receptor analysis   
        8
        |
        45
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416418 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：Radioisotope tracer method, combined with trichloroacetic acid （TCA） precipitation, was used to determine the epidermal growth factor （EGF） in plasma of rabbits. The detection limit for EGF in plasma was 3.0 μg/L. The average recovery of EGF was （97.80±4.94）%. The within day-assay coefficient of variation was （3.23±2.04）%. The assay linearity was determined over the range of 12.5- 400μg/L in plasma and the correlation coefficient was 0.9990. The EGF concentration in rabbits was also determined by the ELISA method, the results of these two methods showed little difference on the whole, but present method was slightly greater than ELISA method.  
        关键词：Epidermal growth factor;Isotope tracer method;TCA method;ELISA method   
        9
        |
        47
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416401 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：Nerve growth factor（NGF） was iodolabelled with chloramine-T method and its distribution and excretion in mice were investigated. The results showed that the highest levels were found in superior cervical ganglia, thyroid, bile, kidneys and adrenals between 20-30 min after injection, and the peak time in most tissues was also found at 20-30 min. About 72.55% of the injected dose was excreted in stool and urine, among them 65.6% was excreted in urine and only 7.0% in stool within 72h after intramuscular injection.  
        关键词：Nerve growth factor（NGF）;(125)I labelled;Distribution;Excretion   
        8
        |
        53
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416541 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：The radioimmunoassay（RIA） has been used to measure epidermal growth factor（EGF） concentration in cancer tissues and non-malignant colorectal epithelial tissues surronding cancer from 40 patients with colorectal cancer. The results showed that EGF was significantly raised in colorectal cancer tissues than in normal epithelium （P<0.001）. This implies that EGF in carcinoma tissue has its specific accumulation.  
        关键词：Epidermal growth factor;Radioimmunoassay;Colorectal cancer;Carcinoma tissue   
        8
        |
        45
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416430 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：Two methods for the synthesis of the [99mTc≡N] intermediates were reported. In the presence of reducer （TPPS or SnCl2） and the donor of N, the mixture of [99mTc≡N] intermediate could be conveniently synthesized but with different composition. On reaction with ligands complexes containing [99mTc≡N]2+ core can be obtained. The change of composition of Intermediates was detected by HPLC at different PH values,and the stability was also evaluated.  
        关键词：Isotope labelling;[99mTc≡N] intermediate;NOEt   
        9
        |
        31
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416444 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：An improved method has been developed for determination of platelet low density lipoprotein receptor （LDL）. LDL was isolated by improved discontinuous density gradient ultracentrifugation. Iodogen method was used for radiolablling and platelet was separated by centrifugation-washing method. LDLR activity was assessed with receptor radioligand binding assay and Bmax. and KD were calculated based on mass action law. The results denoted that this improved method is simple, effective and practical.  
        关键词：Platelet;LDL receptor;Improved assay;RBA   
        8
        |
        39
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416486 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：Radioimmunodetection using 125I and 99mTc-labelled cardiac myosin heavy chain （CMHC） monoclonal antibody （McAb） was undertaken to assess myocardial damage in acute myocardial infarction（AMI） model of rats and dogs. 125I-CMHC McAb was already found in infarcted area at 4h after injection and the highest uptake value was at 8h in rats. The uptake ratio of 125I-CMHC in infarcted area of experimental group to that in normal cardiac area of contral group was 2.86. 99mTc-CMHC McAb was injected intravenously to the dogs with AMI. In SPECT imaging, infarcted area were clearly visualized at 6h post injection. The positive rate was 70%. It is concluded that radiolabelled CMHC McAb can visualize infarcted area by SPECT and the findings suggest that this method is effective for locltization of myocardial damage.  
        关键词：Antibody;Monoclone;Cardiac myosin heavy chain;Acute myocardial infarction;Radioimmunoscintigraphy   
        8
        |
        46
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416512 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：The feasibility and specificity of imaging of myocardial infarct with 99mTc-AMLA was reported. The first group of myocardial infarction（MI） rats were injected 99mTc-AMLA at 24h post MI. The second group were injected with 99mTc-N-IgG for control. For study of the influence of MI onset to uptake of 99mTc-AMLA, the third group with varied duration of MI from 4h to 14d was used.ID%/g and Infracted/Normal uptake ratio（IM/NM） were calculated. One in vitroheart of MI dog was imaged at 24 h after injection of 99mTc-AMLA. The results showed that the infarcted myocardium uptake of 99mTc-AMLA was higher than the normal myocardium. The IM/NM reached 6.68 at 8h in the first group, while non-specific IM/NM of 99mTc-N-IgG was only 1.42 （P<0.01）. There were no significant differences between the ratios of uptake of 99mTc-AMLA in any infarcted duration from 4h to 14d（P>0.05）. The in vitro myocardium imaging showed that the localization of 99mTc-AMLA in myocardium was the same as the blank area of triphenyltetrazolium chloride staining. It was concluded that 99mTc-AMLA is a myocardial imaging agent specific to MI. Thereby, it can be used as a useful infarct avid imaging reagent.  
        关键词：Cardiac myosin protein;Radioimmunoimaging;Monoclonal antibody;Myocardial infarction   
        9
        |
        31
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416473 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：Evaluation of myocardiotoxicity on adriamycin（ADM） with SPECT cardiac blood pool imaging was presented. SPECT was done to monitor left ventricular function in 49 patients with Non-Hodgkin’s Lymphoma and small cell carcinoma, they were treated with ADM of which the accumulation dose were over 300mg/m2. The results showed that parameters of EF, PER and PFR before and after treatment with ADM were 57±6%, 3.08±0.63EDV. s-1, 2.99±0.23EDV. s-1 and 50±11%, 2.25±0.66EDV. s -1, 2.53±0.35EDV. s-1 respectively. The difference of these data are significant （P<0.05）. The larger the accumulative dose of ADM, the more the parameters decrease, among them PFR is the most sensitive one. It was concluded that SPECT measurement of left ventricular function is a sensitive and useful method in monitoring ADM myocardiotoxicity.  
        关键词：Left ventricle function;SPECT;Chemotherapy;ADM;Side effect   
        8
        |
        48
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416585 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：To explore the diagnostic value of SPECT image for children with cerebral infarction （CCI）, comparative research was made on 26 cases undergoing regional cerebral perfusion （rCP） image between SPECT imaging and CT scanning. The results showed that the rCP in the infarct and its distant area was decreased. The positive rate of SPECT and CT were 92.3% （24/26） and 84.5% （22/26） respectively. The difference was not significant （P=0.67, P>0.05）. But, the positive rate of SPECT image 2 days after onset in 9 CCI was 100% （9/9）, significantly higher than 55.6% （5/9） in CT sacnning （P=0.04, P<0.05）. These findings suggested that the SPECT imaging is a sensitive method for the early diagnosis of CCI, and also helpful for observation of the therapeutic effect and evaluation of the prognosis.  
        关键词：SPECT;Cerebral blood flow;Cerebral infarction   
        9
        |
        22
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416460 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：The pharmacological action and hepatobilliary dynamics of the two chief components, a NAA and NTMC of Galle-Donau was reported. Sixty mices were divided into 3 groups. In each group 10mg α-NAA, 10mg NTMC and 0.2mL normal saline, was gastrogavared respectively. 370kBq 99mTc-Mebrofenin was injected intravenously in each mouse and they were killed and the organ radioactivity of each mouse was measured after 5, 15, 30 and 60 minutes, respectively. The results showed that 30 minutes in the group of α-NAA and 5 minutes in the group of NTMC,appeared the increased secretion of 99mTc-Mebrofenin, and the radioactivity in binary system was significant increased. It was concluduced that it demonstrated that Galle-Donau has a significant influence on hepatobilliary dynamics.  
        关键词：Kinetics;Hepatobiliary radiopharmaceuticals;Animal experiment;(99m)Tc-Mebrofenin   
        9
        |
        40
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416500 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：99mTc-EHIDA radionuclide hepatobiliary imaging was performed to evaluate the hepatobiliary functional changes one to two weeks after cryosurgery in porta hepatis of porcine. The imaging shown that the radioactivity of cryosurgical hepatic region was decreased or absent. The time of appearence of radioactivity in intestines was significantly prolonged, and was statistically significant as compared with control group. The binary tract system was damaged. It was suggested that the prefect protection of binary system should be accomplished in the cryosurgery of porta hepatis to avoid severe complications.  
        关键词：(99m)Tc-EHIDA;Radionuclide imaging;Cryosurgery   
        8
        |
        29
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416531 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：27 cases of lung tumor patients performed SPECT imaging 48 to 120 hours after injection of 185 MBq 67Ga. 19（70%） showed positive result and undertook operation.11 of the 19 patients performed SPECT imaging again 3 months after operation, 4 of 11 patients appeared new metastases. 6 months later 2 positive cases and 4 negative cases of the above 11 patients repeated 67Ga iamging. In positive cases, the metastases increased, whereas in negative cases, new metastases discovered. All these result was the same as X-ray and CT. It was concluded that 67Ga imaging is a simple and accurate method for the detection of recurrence, monitoring of therapeutic effect and treatment regime in lung tumor patients. 67Ga imaging has its good clinical value.  
        关键词：Lung tumor;(67)Ga;Imaging;Follow-up   
        7
        |
        19
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416571 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：To explore the relationship between PRL, GH（RIA）, GH（RRA） serum levels changes and the onset and development of disease in patients with SLE, and to understand the hormonal changes in active and inactive phase of disease, 28 cases and 20 controls were studied. The results showed that anti-ds-DNA serum level in active and inactive phases was higher than those in controls （P<0.01）, and also there was significant difference between active and inactive phase （P<0.01）. Morever,there is significant difference in PRL level between the patients in active phase and the controls （P<0.05）, also in GH serum level between the patients and the controls （P1<0.01） and the patients in inactive phase （P2<0.05）. This study indicated that the higher serum level of PRL, GH（RIA）, GH（RRA） is correlated with some autoimmune characteristics of SLE, especially in its active phase. Therefore it gives some help for clinical study of SLE.  
        关键词：Systemic lupus erythematosus;Prolactin;Growth hormone   
        8
        |
        39
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416617 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：通过甲状腺结节模型模拟实验研究评价B超、CT、核素显像对甲状腺结节诊断的价值。结果显示：B超、CT和核素显像的结节检出率分别为90．9％、91.4％、84．8％，准确率分别为92％、100％、100％，但B超有7.6％的误检率。核素显像中。针孔准直器无论是平面还是断层显像均优于平行孔准直器，尤其是对小结节（≤5mm）的诊断针孔断层可提高小结节的检出率。  
        关键词：Thyroid;Nodules phantom;Nuclear-imaging;Ultrasound;CT   
        8
        |
        98
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416557 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：188Re-HEDP have been prepared with 188Re eluted from a 188W/188Re generator.The best labelling condition is 5 mg HEDP, 2.0mg SnCl2, and 1 mg Vitamin C, PH2.0.Labelling yield is greater than 95%. The in vitro stability of 188Re-HEDP is good in 12 hours. By adding some adjunct reagent in preparation, femur uptake raised to 7.86 ±1.62%/g after 4 hours. As compared with control uptake 1.92±0.61%/g in mice （P<0.01）. Clear whole body images of rabbit has been obtained. Thereby 188Re-HEDP is a good potential candidate for the treatment of bone tumor.  
        关键词：Bone tumor;Radionuclide therapy;(188)Re-HEDP   
        7
        |
        53
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416600 false
        发布时间：2021-04-09
      • Issue 11, (1998)
        摘要：90Y generator was made by absorption 90Sr-90Y equilibrium system to Dowex 50 resin. 90Y was obtained by elution with sodium citrate buffer solution. The 90Y was mixed with Biou-Rex 70 resin, high binding rate were achieved when the pH value was more than eight and the mixing duration was 4 h.90Y labelled resin was injected through hepatic artery catheter into the right lower lobe of liver in dog. The total blood concentration of 90Y was estimated to be 1% of total dose administered.After 2 weeks, X-CT showed that the administered liver lobe observed liquified necrosis and liver atrophy grossly, also liver cell die away extensive1y in pathological sections.These prelimenary findings suggested that with selective administration of hepatic artery catheterization, radiolabelled resin was an effective and safe radioinjury reagent, and can be used for tumor radiotherapy.  
        关键词：(90)Y;Resin;Radiotherapy   
        9
        |
        22
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 8416633 false
        发布时间：2021-04-09
      0