最新刊期
卷 0 ， 期 0 ， 2020
- 摘要：To investigate the preparation of 125 I- somatostatin analogy (RC-160) and its binding characteristics to NCI-H446 cell receptors. RC-160 was labeled with Na 125 I using chloramines-T method, then purified by C 18 reverse phase chromatography and examined by silica chromatographic paper. Time-binding correlation test, temperature-binding correlation test, saturable binding test and competition binding test between NCI-H446 cells and 125 I-RC-160 were carried out. The labeling rate of 125 I-RC-160 was 54.34%. The specific activity of 125 I-RC-160 was 1.85TBq/mmol·L -1 . The radiochemical purity was 92%. The value of K d and B max was 1.71×10 -10 mol/L and 1.06×10 5 site /cell respectively. The labeled 125 I-RC-160 could be used for receptor analysis and the iodination of RC-160 does not change its binding to somatostatin receptor. The results demonstrated that the binding of 125 I-RC-160 to NCI-H446 cell possessed the reversibility, specificity, saturability, and was time and temperature dependent. The NCI-H446 cell line expresses somatostatin receptors with high density and affinity. The binding is in accordance with single site system.关键词：Somatostatin;Receptor;Small cell lung cancer
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发布时间：2021-03-12 - 摘要：To evaluate the value of positron emission tomography (PET) imaging with 18 F-FDG in differentiation of benign and malignent pancreatic mass. 12 patients with pancreatic occupying lesion diagnosed by ultra sond or CT/MR including 7 pancreatic cancer and 5 pancreatitis underwent 18 F-FDG PET imaging. Visual interpretation and semiquantitative analysis by calculating the tumor/liver ( T/L ) ratio based on ROI were performed on attenuation corrected images. 9 positive findings were detected. Among them, 7 were confirmed to be cancer, but the other 2 were mass-forming pancreatitis. Final diagnoses of the 3 patients with negative findings were confirmed to be pancreatitis. The mean T/L ratio was 2.58±0.95 in pancreatic cancer, significantly higher than that in pancreatitis (1.29±0.87) ( p =0.037). With a T/L ratio cutoff value of 1.5, all 7 cancer patients were correctly categorized. However, one pancreatitis had T/L ratio higher than 1.5. 18 F-FDG PET imaging was a potential reliable method in differentiating benign or malignant pancreatic mass with high negative predictive value, but the specificity was limited. Semiquantitative analysis may improve the accuracy of the diagnosis.关键词：Positron emission tomography(PET);Flruorodeoxyglucose;Pancreatic cancer;Pancreatitis
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发布时间：2021-03-12 - 摘要：The relationship between the homogeneity of irradiation field created by magnetic scan in heavy-ion radiotherapy and the size and scanning step of beam spot was investigated by computer simulation. Two formulae for the beam intensity and irradiation time calculation respectively were derived from the slice-by-slice idea of heavy-ion radiotherapy. The simulation result shows that the homogeneity better than 95% can be achieved when the scanning step is smaller than the half width of beam spot. The suitable beam intensity, the maximum scanning speed and the minimum spot stay time were obtained as 0.48nA, 1000cm/s, 0.24ms respectively.关键词：Heavy-ion radiotherapy;Homogeneity of irradiation field;Magnetic scan;Maximum scanning speed;Minimum spot stay time
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发布时间：2021-03-12 - 摘要：The purpose of this article is to study the preparation and biodistribution in animals of dopamine D2 receptor ligand 131 I-epidepride. Epidepride was 131 I radioiodinated with hydroperoxide or chloramine-T method. The purity and stability of 131 I-epidepride were studied by HPLC and TLC, the dynamic distribution in brain and critical organs of SD rats were studied. The radiolabelling yields of 131 I-epidepride were 97.4% and 52.9% respectively. With no more purification, the hydroperoxide method is better than the chloramine-T method. After 4 hours in room temperature, the radiochemical purity of 131 I-epidepride was still over 90%. The striatum uptake was good, the striatum/cerebellum ratio reached 237:1 at 320min. Among the critical organs,lung had the first and the highest uptake. All organs had quick clearance ( T 1/2 <4h). The radioactivity in thyroid increased with time. The results show that 131 I-epidepride has a high striatum uptake and a high striatum/cerebellum ratio. 131 I-epidepride is a good dopamine D2 receptor agent关键词：Dopamine D2 receptor;131 I-epidepride;Biodistribution
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发布时间：2021-03-12 - 摘要：The purpose of this work is to measure the pharmacokinetic parameters of r-Sak in rats as well as the biodistribution and the excretion of r-Sak in rats. 125 I-r-Sak was applied for the purpose. The results showed that the pharmacokinetics of 125 I-r-Sak following intravenous injection with two doses (70μg/kg and 840μg/kg) in rats was in correspondence with 2-compartment-model. The T 1/2(α) were 5.47±2.13 and 8.45±4.50min and T 1/2(β) were 96.15±22.29 and 58.78±32.79min for the two dose groups. AUC were 0.895±0.455 and 38.36±5.181μg·min·mL -1 . There were no marked differences of T 1/2(α) and T 1/2(β) between the two dose groups ( P >0.05), but the AUC increased markedly with the dose increase ( P <0.05). The biodistribution of 125 I-r-Sak was wide in rats. Radioactivity was highest in kidney, followed in order by plasma, liver, lung, spleen, heart and brain. Excretion experiment showed that the sum of the accumulative excretion of the radio-material plus the enrichment of the radio-material in thyroid was approximately 80% in 96h.关键词：I-r-Sak;Pharmacokinetics;Biodistribution;Excrement
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发布时间：2021-03-12 - 摘要：The active fractions of fructus trichosanthes cortex with antagonistic effect were studied by 45 Ca isotopic tracer technique- 45 Ca transmembrane influx technique. The fructus trichosanthes cortex was first extracted by 30-100% ethyl alcohol. The calcium antagonism of the extract obtained by 70% ethyl alcohol was found to be the highest. This extract was further extracted by petroleum ether, then isolated on Silica gel columm chromatography with elution of ethyl acetate and 95% ethyl alcohol. It was found that the eluates have five fractions. Among them, the fifth fraction had the strongest Ca 2+ antagonistic effect. In contrast, the third fraction has obvious activiating effect, but no antagonistic effect.关键词：Ca;Isotopic tracer technique;Fructus trichosanthes;Ca 2+ antagonist;Coronary heart disease
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发布时间：2021-03-12 - 摘要：To evaluate the clinical value of combined 153 Sm-EDTMP and Yunke therapy in metastatic bony pain. 210 patients with metastatic bony pain were treated using 153 Sm-EDTMP or combined with Yunke. The effect of treatment were observed. The total pain relief rate in single 153 Sm-EDTMP group was 83.7%, whereas in combined 153 Sm-EDTMP with Yunke group was 94.7%. The latter was markedly higher than the former. The clinical value of combined 153 Sm-EDTMP and Yunke therapy group in relief of metastatic bony pain is obvious. And it was safe and no significant adverse reaction was observed. The pain relief effect of combined 153 Sm-EDTMP and Yunke therapy is better than the single 153 Sm-EDTMP therapy.关键词：Metastatic bone cancer;153 Sm-EDTMP;Yunke;Pain relief
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发布时间：2021-03-12 - 摘要：To study the relationship between diabetic impotence and sex hormones as well as sex hormone receptors. 32 diabetic impotent patients, 32 diabetic patients with normal sex function, 32 impotent patients without diabetes, and 40 healthy men were enrolled. The plasma sex hormone levels were examined by radioimmunoassay, and sex hormone receptors in white blood cells by radioreceptor assay. Compared with healthy men and impotent patients without diabetes, PRL levels in both diabetic impotent patients and diabetic patients with normal sex function increased markedly, T and AR levels decreased, and the ratio of E 2/T and ER/AR increased. Compared with diabetic patients with normal sex function, while there was no significant difference in PRL, T and E 2/T ratio, the AR level of diabetic impotent patients further decreased, and the ER/AR ratio further increased. Negative correlation was found between age and AR as well as T. The decline of AR and the increase of ER/AR ratio might be one main cause of diabetic impotence. And the decline of T and AR might be an important cause of the increase of diabetic impotence incidence with age.关键词：Diabetes;Impotence;Receptors;Androgen;Testosterone;Prolactin
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发布时间：2021-03-12 - 摘要：Evaluation of the immunological properties and safety of 99m Tc-C 50 CEA McAb labelled by 2-IT was reported. C 50 was modulated chemically by 2-IT and labelled by 99m Tc with stannous reduction. The immunological activity, stability and safety were studied, and radioimmunoimaging were performed in human colon tumor bearing mice. The results showed that the immunological activity, the best labelling rate of chromatography and colloid inclusion were 40.2%-73.2%, 94.1% and 3.8% respectively, and radiochemicl purity maintained over 90% within 12h after labelling. Labelling products was pyogen free and no toxicity was found. Radioactivity was highly accumalated at tumor site of 6h after injection human colon tumor bearing nude mice, T/NT ratio was 3.46-4.68 at 24h after 99m Tc-C 50 injection. All the findings suggested that preconditioning with 2-IT to C 50 can improve labelling of 99m Tc,and minimize the injury of protein. The labelling method is simple and safe, and can be effectively used for clinical study.关键词：Carcinoembryonic antigenn;Monoclonal antibody;Radioimm-unoimaging
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发布时间：2021-03-12 - 摘要：The Tc-carbonyl complexes of tryptophan and histidine were synthesized by two-step method. The yielded complexes were found by paper electrophoresis to be electrically neutral in three buffer solutions (pH=4.7, 7.4, 9.2). Their possible structures were postulated based on ab initio MO calculations. The biodistribution in mice bearing S180 tumor demonstrated that the Tc-carbonyl complex of histidine showed good stability in vivo and quick clearance, selectively accumulated in tumor. The tumor/muscle ratio attained 3 to 4. However, the complex of tryptophan showed poor stability in vivo and slow clearance, and retained for a long time in organs and tissues. It also accumulated in tumor to some extent. The tumor/muscle ratio attained 2 to 3. The labeling of proteins and polypeptides with the Tc(I)-carbonyl complex was also discussed.关键词：Tryptophan;Histidine;Tc-carbonyl;Biodistribution
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发布时间：2021-03-12 - 摘要：To investigate the levels of carbohydrate antigen 19-9 in the urine samples of the patients with bladder cancer and to evaluate its clinical diapnostic value. Urine samples were taken from 30 patients with bladder cancer, 53 with benign, 22 with malignant urological diseases and 35 with malignant tumors from other systems, together with 30 normal subjects which have no any history of cancers or other urological diseases. CA19-9 was assayed by Chiron Diagnostic Corporation ACS:180SE. The CA19-9 level of the group with bladder cancer was 159.0±128.0U/mL, while that of the group of control was 12.4±8.4U/mL. The critical points were determined as the mean value of the group of control ±1.96 SD , then >28.9U/mL was considered as positive. The diagnostic sensitivity for bladder TCC were 86.7% and specificity were 68.2%. CA19-9 level in the bladder cancer group was significantly different from that of control group ( P <0.001), and also different from those of other groups. The urine CA19-9 level in the group of benign urological diseases was 53.9±77.9%, significantly higher than that of control ( P =0.001), but not significantly different from those of the group of other urological cancers and other systems cancers. Preliminary study indicates that CA19-9 urine samples study is a non-invasive auxiliary index for the clinical diagnosis of bladder cancer. The method is simple and useful. But the interference from other benign and malignant diseases as well as gene-types should be considered in clinical practice.关键词：Bladder cancer;Carbohydrate antigen 19-9;Tumor marker
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发布时间：2021-03-12 - 摘要：To check the performance of SPECT, γ-camera and scanner in Shanghai, and to promote applying quality of nuclear medical imaging instrumentation. (1) Performance phantom (76-823 source tank, 76-824 set of 3 inserts): It permits measurements of the resolution of "hot" lesions in a "cold" field, "cold" lesions in a "hot" field, linearity, uniformity of response to a uniform field. (2) Hoffman brain phantom (model No.9000): Providing a truly three-dimensional model of the radioisotope distribution in the normal patient brain. The size and structure of the phantom including the gray and white matter subsections, are representative of studies in emission tomography. The phantom simulates the 4:1 gray to white matter uptake ratio, that is normally seen in studies such as 123 I and 99m Tc brain perfusion imaging in SPECT. (3) Thyroid phantom: Composing of three "cold" lesions whose diameters are 8,10 and 12mm and one "hot" lesion whose diameter is 10mm. Left lobe is 9mm thick while right one 18mm thick. There are 22 SPECT scanners including 8 double-detector SPECT and 14 single-detector SPECT. Of the 22 SPECT 16 were qualified in uniformity. The "hot" lesions were 9-10mm with Double-detectors, 11-12mm with single-detector; while the "cold" lesions were about 10mm and 11-12mm respectively. The brain imaging is not very good generally. 50% of γ-cameras were not qualified in uniformity. Scanners did not detect the thyroid phantom "cold" or "hot" lesion. According to the quality control results, the applying performance of nuclear medicine imaging instrumentation is relative satisfactory in Shanghai.关键词：Quality control;SPECT;γ-cameras;Scanner
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发布时间：2021-03-12 - 摘要：To study a new method for predication of portal vein pressure (PVP) by hepatic perfusion imaging. 25 hepatocirrhotic cases and 13 normal controls were performed the hepatic perfusion imaging. According to two compartmental model the values of portal vein indexes (PVI) was calculated using curve slope, area and hepatic heart perfusion ratio methods etc. The relationship of PVI with different method to PVP was also observed. All PVI by three methods in hepatocirrhosis were higher than those in normal controls ( P <0.01), and also positively correlated with the PVP, their correlated coefficients was 0.79, 0.60, 0.68 respectively. Among them the slope method was most markedly significant than nomal control and closely correlated with PVP. PVI can sensitively reflect the changes of portal vein blood flow. And it was an atraumatic, simple method for the evaluation of PVP.关键词：Hepatic perfusion imaging;Portal vein pressure;Portal vein index;Hepatocirrhosis
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发布时间：2021-03-12 - 摘要：To evaluate clinical value of single or combined tumor marker detection CY21-1, CEA, CA15-3 and SCC in the diagnosis of lung cancer. There was retrospective analysis of 87 lung cancer in-patients, all of them was confirmed by pathology. Result showed: (1) Sensitivity of CY21-1, CEA, CA15-3 and SCC by single detection in diagnosing lung cancer was 59.8%, 39.1%, 44.8%, 18.4%, respectively. (2) Sensitivity of groupⅠ (CY21-1+CEA) was 78.2%; sensitivity of group Ⅱ (CY21-1+CEA+CA15-3)was 88.5%; sensitivity of group Ⅲ (CY21-1+CEA+CA15-3+SCC) was the same as group Ⅱ. In the diagnosis of lung cancer, the combined detection with CY21-1, CEA, CA15-3 was an ideal selective combination.关键词：Tumor markers;Lung cancer;Diagnosis;Detection
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发布时间：2021-03-12 - 摘要：healthy women aged 31-72 years participated in the study, include 64 sexual maturity women aged 31-52 were enrolled in four groups based on age, and 67 postmenopausal women, the years of menopause from 1 month to 23 years, were also enrolled in four groups based on duration of menopause. The bone mineral density of the lumbar-spine and femur were measured using dual-energy X-rays absorptionmetry. At the same time, the serum E 2, FSH, BGP were detected by radioimmunoassay; the serum AKP, calcium, phosphate were detected using automatic biochemistry instrument. The results were: the BMD of lumbar-spine and right femur were decreased, and associated with increasing age and the duration of menopause, especially after menopause. Significant positive correlation was noted between BMD and E 2. Serum AKP and BGP levels were higher in postmenopausal women than those of sexual maturity women. The serum calcium level was increased significantly soon after menopause, then decreased to normal level, and maintained in this level. The serum phosphate level had no difference in these groups. Therefore BMD, serum E 2, BGP and AKP determination have some value for the early diagnosis of postmenopausal osteoporosis and prediction of bone tracture also.关键词：Dual-energy X-rays absorptionmetry;Bone mineral density;Estrogen;Postmenopausal osteoporosis
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发布时间：2021-03-12 - 摘要：Acquisition of both stressed and rest myocardial perfusion simultaneously is possible when dual-isotope technique is used. And using this technique we also can obtain data of myocardial perfusion and metabolism imaging with different clinicge significance under the same physiological condition. The clinical application of dual-isotope in nuclear cardiology and its scatter correction was reviewed in this article.关键词：Dual-isotope;Nuclear cardiology
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发布时间：2021-03-12 - 摘要：This paper describes in detail the advances in monoclonal antibodies for radioimmunotherapy since 1990. It introduces the radionuclides, monoclonal antibodies including their dosimetry, toxicity and radiobiological characteristics in radioimmunotherapy. Finally it discusses the problems, potential solutions and future directions in developing monoclonal antibodies for radioimmunotherapy.关键词：Radioimmunotherapy;Monoclonal antibody;Radioisotope
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