The preparation of      <sup>125</sup>I-RC-160 and its   in vitro   binding characteristics   to the receptors of NCI-H446 cell line

LI Fujun  WANG Jing  DENG Jinglan

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The preparation of ¹²⁵I-RC-160 and its in vitro binding characteristics to the receptors of NCI-H446 cell line

更新时间：2021-03-12

- The preparation of ¹²⁵I-RC-160 and its in vitro binding characteristics to the receptors of NCI-H446 cell line
- NUCLEAR TECHNIQUES Issue 5, (2002)
- 作者机构：
  
  1. 第四军医大学西京医院
  2. 第四军医大学口腔医院,西安,710032
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助 (39870 2 18)课题
- DOI：
  CLC： R817.1
- Published：01 May 2002
- 稿件说明：
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[1]李富军,汪静,邓敬兰,李焰.~(125)I-RC-160的标记及与NCI-H446细胞体外受体结合特性的研究[J].核技术,2002(05):321-326.

LI Fujun WANG Jing DENG Jinglan. The preparation of ¹²⁵I-RC-160 and its in vitro binding characteristics to the receptors of NCI-H446 cell line[J]. Nuclear techniques, 2002, (5): 321-326.
[1]李富军,汪静,邓敬兰,李焰.~(125)I-RC-160的标记及与NCI-H446细胞体外受体结合特性的研究[J].核技术,2002(05):321-326. DOI：

LI Fujun WANG Jing DENG Jinglan. The preparation of ¹²⁵I-RC-160 and its in vitro binding characteristics to the receptors of NCI-H446 cell line[J]. Nuclear techniques, 2002, (5): 321-326. DOI：

摘要

为探索高比活度12 5I -生长抑素类似物（RC - 160 ）的制备及与小细胞肺癌（SCLC）细胞株-NCI-H4 46体外受体结合特性。12 5I -RC - 160采用氯胺T法标记

C18反向色谱柱纯化

硅胶纸层析检测。12 5I-RC - 160与NCI -H4 46细胞进行体外结合实验、饱和结合实验和竞争结合实验。12 5I -RC - 160标记率为 5 4.34 %

比活度 1.85TBq/ （mmol·L- 1）

放射化学纯度（RCP）为 92 % ;Scatchard作图提示

Kd 为 1.71× 10 - 10 mol/L

Bmax为 1.0 6× 10 5个 /细胞。制备的12 5I -RC - 160达到体外受体配体结合分析的标准 ;12 5I-RC - 160与NCI -H4 46的结合具有温度时间依赖性、可饱和性、可逆性及特异 ;12 5I -RC - 160与NCI -H4 46细胞生长抑素受体的结合符合单位点系统。

Abstract

To investigate the preparation of 125 I- somatostatin analogy (RC-160) and its binding characteristics to NCI-H446 cell receptors. RC-160 was labeled with Na 125 I using chloramines-T method

then purified by C 18 reverse phase chromatography and examined by silica chromatographic paper. Time-binding correlation test

temperature-binding correlation test

saturable binding test and competition binding test between NCI-H446 cells and 125 I-RC-160 were carried out. The labeling rate of 125 I-RC-160 was 54.34%. The specific activity of 125 I-RC-160 was 1.85TBq/mmol·L -1 . The radiochemical purity was 92%. The value of K d and B max was 1.71×10 -10 mol/L and 1.06×10 5 site /cell respectively. The labeled 125 I-RC-160 could be used for receptor analysis and the iodination of RC-160 does not change its binding to somatostatin receptor. The results demonstrated that the binding of 125 I-RC-160 to NCI-H446 cell possessed the reversibility

specificity

saturability

and was time and temperature dependent. The NCI-H446 cell line expresses somatostatin receptors with high density and affinity. The binding is in accordance with single site system.

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Related Author

汪静

金河天

王连刚

武胜昔

邓敬兰

朱瑞森

袁志斌

CHEN Libo YUAN Zhibin ZHU Ruisen

Related Institution

Xijing Hospital,The Fourth Military Medical University

沈阳军区２０２医院!沈阳１１００００

第四军医大学!西安７１００３２

上海交通大学附属第六人民医院核医学科

Department of Nuclear Medicine,Shanghai Sixth People’s Hospital,Shanghai Jiaotong University

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The preparation of 125I-RC-160 and its in vitro binding characteristics to the receptors of NCI-H446 cell line

DOI：

摘要

Abstract

关键词

Keywords

references

The preparation of ¹²⁵I-RC-160 and its in vitro binding characteristics to the receptors of NCI-H446 cell line