最新刊期
      卷 0 ， 期 0 ， 2020
      • WANG Jing WANG Xiqing WANG Liangang LI Fujun DENG Jinglan
        Issue 11, (2002)
        摘要：A method for the iodination of peptide RC-160 with high efficiency was developed. RC-160 was iodinated with N-bromosuccinimide (NBS) as oxidant, the conventional chloramine T (Ch-T) method was used as control. The labelling condition of NBS method was optimized and radiolabelled conjugate 125I-RC-160 was assessed as follows: no further purification was needed, the measured labelling yield of 125I-RC-160 was 92% and the specific activity was 1.95×10 12Bq/mmoL. The yield increased as the amount of NBS increased. The optimal ratio of RC-160(μg): 125I(MBq):NBS(μg)was 3:7.4:1. For Ch-T method, the labelling yield is 56% and specific activity was 0.65×10 12Bq/mmoL, but after purification by SepPaK-C 18, the labelling yield may reach as high as 92%. 1h after injection, radioactivity in blood decreased by 87.2%. No obvious concentration of 125I-RC-160 in thyroid or kidney was observed.  
        关键词：Somatostatin analogy;Iodination;Radiolabelled   
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        发布时间：2021-03-12
      • HUANG Gang REN Xiaoqing CHEN Jianwei MAO Jialiang HUANG Dingju 1
        Issue 11, (2002)
        摘要：To compare the effects of β-particle radiation on proliferation of smooth muscle cells and endothelial cells, and to explore the methods for protecting endothelial cells during endovascular radiotherapy, the smooth muscle cells and endothelial cells cultured in vitro were irradiated by 188Re with different single doses. By means of proliferation cell count, cell cycle progression analysis, 3H-TdR incorperation, the effects of β-particle on proliferation of smooth muscle cells (SMCs) and endothelial cells (ECs) were studied and compared. SMCs proliferation, but not ECs, was significantly inhibited by β-particle irradiation with dose of 5.2Gy. SMCs and ECs were inhibited similarly by β-particle irradiation with doses >10.3Gy and <20Gy. ECs appeared to be more radioresistant than SMCs at a lower dose. A safety window of treatment with 188Re at a dose of 5.2Gy was found between the smooth muscle cells and endothelial cells cultured in vitro.  
        关键词：188Re;Radiation;Proliferation;SMCs;ECs   
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        发布时间：2021-03-12
      • SHI Hongcheng CHEN Shaoliang YAO Zhifeng ZHU Weimin LIU Wenguan
        Issue 11, (2002)
        摘要：To study the characteristics of common myocardial tomography artifacts so as to increase the diagnosis accuracy, 132 patients with myocardial perfusion were reviewed. With careful recognition of artifacts, the patients were re-diagnosed and compared with previous results. It was found that attenuation artifacts and motion artifacts were found frequently in thallium SPECT images. Artifacts caused by thoracic wall were frequent in female and showed a fixed defect in anterior or lateral wall; attenuation artifacts caused by left hemidiaphragm were always found in male and showed that the inferior wall became thinner gradually from apex to bottom in vertical long axis slices. The coexistence of defects and hot areas was the characteristics of motion artifacts. Artifacts caused by non-target organs, liver and/or gastrointestinal tracts, were frequently seen in 99mTc- sestamibi SPECT image. By recognizing the artifacts, misdiagnosis rate decreased significantly from 13.6% to 6.8%. Our studies show that all kinds of artifacts have their prevalence rate and imaging feature, the artifacts could be eliminated effectively by careful analysis and some correction measures.  
        关键词：Artifacts;Myocardial perfusion SPECT;Diagnosis accuracy   
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        发布时间：2021-03-12
      • WANG Shuxia DIAO Yao PEI Zhuguo
        Issue 11, (2002)
        摘要：To quantify protected effect of norepinephrine (NE) induced delayed phase of ischemic preconditioning, the relationship between cardioprotection and superoxide dismutase(SOD) was studied. A total of 50 rats were randomly divided into three groups:NE group, I/R group and normal group, 3.1 μmol/kg NE was injected into abdomen 24 hours before surgery as a trigger, both NE and I/R group underwent main left coronary artery occlusion for 30 minutes then reperfusion for 120 minutes. Ischemic risk area, infarct area and nonischemic area were identified by TTC, Evan’ s blue staining methods and calculated by Metamorph Imaging System software. Serum Cu 2+, Cytosol Cu 2+, Zn 2+-SOD content and activity, and activity of mitochondrial Mn-SOD activity were detected by radioimmunoassay and method xanthosine oxidase separately. Results showed that: 1) infarct size was reduced by 33.4% in preconditioned heart; 2) mitochondrial Mn-SOD activity was higher in preconditioned myocardium; 3) no significant difference about the activity and content of Cu 2+,Zn 2+-SOD either in serum or in myocardium was found. The above phenomena imply that preconditioned cardioprotection might be only related to mitochondrial Mn-SOD, mitochondrial Mn-SOD might be the final effector of NE induced preconditioning .  
        关键词：Reperfusion injury;Ischemic preconditioning;Myocardium;Mitochondrium;Mn-SOD;Radioimmunoassay   
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        发布时间：2021-03-12
      • TANG Ganghua 1,2 ZHANG Lan 1 TANG Xiaolan 2 WANG Mingfang 2 WANG Yongxian 1 HUANG Zuhan 2 YIN Duanzhi 1 1
        Issue 11, (2002)
        摘要：Fluoro-L-DOPA ( 18FDOPA), an analogue of L-DOPA is a tracer for presynaptic dopamine function in cerebral studies with PET. In order to use it safely and effectively in clinical study, quality control of 18FDOPA was studied. Determination of the enantiomeric purity of 18FDOPA by HPLC method using a chiral mobil phase and reversed-phase C18 column was described. All quality criteria of 18FDOPA met the requirements of the local pharmacopoeia. The relevant details involved in quality control of 18FDOPA might be useful in the future development of new radiopharmaceuticals for PET.  
        关键词：Fluoro-L-DOPA;Quality control;Entiomeric purity;HPLC method using a chiral mobil phase and reversed-phase C18 column   
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        发布时间：2021-03-12
      • GAO Kejia WANG Jianhua YE Zhiwei LIU Hongqi HUANG Xiang CHEN Hailin YANG Guang ZHANG Shuzhong JIANG Shujun HE Simei CHEN Ronggen
        Issue 11, (2002)
        摘要：The radiation from 32P-GTMS was used to suppress the proliferation of prostate mesenchymal cell and improve the symptom of dysuria. (1) 25mg/kg of testosterone propionate were intramuscularly injected into 5 male healthy dogs three times a week for 30 days to establish the prostate proliferation model. Under the guidance of ultrasonography 9.25-74mBq/mL of 32P-GTMS were injected and 30, 60, 90 days post injection biopsy were examined to get the data of prostate destruction and its shrinking. Another 2 dogs were not treated with 32P-GTMS, but injected with testosterone propionate to serve as controls. Then we compare the rate of proliferatioe of prostate proliferation. (2) According to the data from dogs, 111±18.5mBq of 32P-GTMS were injected to 34 prostate proliferation patients under the guidance of ultrasonography and the prostate volume, urine flow, remnant urine and I-PSS scores were recorded at 30, 60 and 90 days post injection. In dogs, 30 days after the injection of testosterone propionate, the prostate were enlarged more than 2 times. After treatment with 32P-GTMS, 5 dogs all showed cytometaplasia and mesenchymal cell infiltration. After 90 days the average shrinking rate was larger than 43.4%, the average shrinking rate of compare group was loss than 13%. The average shrinking rate of 34 cases of prostate proliferation patients are 17% and the urine flow increased by 41%, remnant urine were decreased by 74% and I-PSS score decreased by 31%,(P<0.01). Pathological sections showed degeneration and atrophy of the prostate measenchymal cell. The method is an easy and non- painful way that can shrink the prostate and make the emiction freely.  
        关键词：BPH;Dog;Animal model;32P-GTMS;Treatment   
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        发布时间：2021-03-12
      • SANG Shibiao WU Yiwei ZHANG Wei JIANG Yimin CHEN Guangxiang DONG Tianhua
        Issue 11, (2002)
        摘要：Nine mature and healthy dogs were divided into 4 groups randomly. The first group consists of 3 dogs, and the rest groups, 2 dogs. Self-control way was used. The left ilium was the experimental site, where the bone piece with sartorial muscle attached was cut from ilium. The right side served as control, where the graft bone from ilium was wrapped up with silica gel, then fixed subcutaneously. The pedicled bone pieces were retrieved in 2, 4, 6, 8 week after surgery, the size of graft bones from two sides were the same. Survival range, osteogenic formation of muscle-pedicle bone were examined by 99mTc-MDP bone imaging and histological method to evaluate the clinical value of treatment of ischemic osteonecrosis of the femoral head with muscle-pedicle bone grafting. The results were as follows: 1. The part of bone attached by the sartorial survived, but osteonecrosis was observed at the location of 1.2-1.7 cm from attached point of muscle. 2. There were two forms observed in the course of osteogenic activation of muscle-pedicle bone. Under the reduction of mechanical stimulation and blood supply, bone marrow tissue was replaced by fiberogranulation tissue and new capillary in the survival part, while the survival bone of proximal part grew distally and gradually replace the dead bone of distal part. 3. Histological examination revealed that small amounts of fiberogranulation tissue and new capillary appeared in 2nd week, primary trabeculae appeared in 6th week, and in 8th week, some trabeculae recovered their normal appearance and osteogenic cell reduced remarkably. 4. Survival range of muscle-pedicle graft bone was directly proportional to the width of muscle pedicle. Therefore, radionuclide bone imaging and histological examination demonstrated that muscle-pedicle grafte bone was a piece of bone with relatively poor blood supply, its effect in repairing necrosis and collapse of the femoral head is limited. 99mTc-MDP bone imaging may be an effective and first choice method for the early monitoring of the muscle-pedicle bone graft osteogenic activation.  
        关键词：Muscle-pedicle graft bone;Radionuclide bone imaging   
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        发布时间：2021-03-12
      • WU Chunying LIN Xiangtong
        Issue 11, (2002)
        摘要：The biodistribution of 125I-BMIPP in SD rats and the effect of metabolic intervention on myocardial uptake of 125I-BMIPP were reported. The maximal myocardial uptake of 125I-BMIPP in fasted rats was 5.70ID%/g and remained constant during 1h. The myocardial uptake was 2.18ID%/g at 120min postinjection. The ratio of myocardium to blood, myocardium to liver and myocardium to lung at 30min were 3.40, 2.64 and 2.88 respectively. The liver and lung showed a low uptake and rapid clearance of 125I-BMIPP. Toxicity test revealed that the tolerable dose per kg in mice was as high as 500 times that of the recommended dose for clinical application, when the in vivo and in vitro test showed that the binding of 125I-BMIPP to HSA was stable up to 2 h. Partition coefficients (logP) at pH 7.0 and 7.4 were 1.93 and 1.68 respectively. The study of metabolic intervention showed that myocardial uptake of 125I-BMIPP in the glucose-insulin group was significantly increased (P<0.05) than those of the normal control.  
        关键词：I-BMIPP;Fatty acid;Metabolic intervention;Glucose;Insulin;Sodium lactate   
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        发布时间：2021-03-12
      • YUAN Zhibin YU Yongli LU Hankui ZHU Jifang ZHU Ruisen
        Issue 11, (2002)
        摘要：The well differentiated thyroid cancer patients had a better prognosis. High-dose radioiodine therapy may induce immediate and long-term side effects to patients. The aim of this paper is to investigate the side effects after high-dose radioiodine therapy on thyroid cancer patients and the relation with cumulative therapy dosage. According to the common side effects of 131I therapy, 10 questions was chosen to set up a standard questionnaire. Patients were followed up by senior nuclear medicine physicians and the results were analyzed and compared. The results showed that the late symptom of sialadenitis and severe dry mouth were intensively related with cumulative therapy dosage. It is suggested that during the therapy certain necessary means should be adopted to reduce the potential side effects and improve the patients’ life quality.  
        关键词：Thyroid cancer;Radioiodine therapy;Side effects   
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        发布时间：2021-03-12
      • JI Shundong 1 FANG Wei 3 WANG Feng 2 LIU Xiujie 3 SHI Rongfang 3 WU Jinchang 2 RUAN Changgeng 1 1
        Issue 11, (2002)
        摘要：To establish a new method of preparation of 99mTc -labelled SZ-51 for radioimmunoimaging of thrombi and to evaluate the value of 99mTc-SZ-51 in detecting pulmonary embolism and distinguishing fresh and old thrombi. SZ-51 was modified with 2-iminotholane and labelled with 99mTc-GH using ligand exchange method. Labelling yield and immunoreactivity were determined by instant thin-layer chromatography ( ITLC) and enzyme linked immunosorbent assay ( ELISA) respectively. The kit was produced using the optimal labelling conditions. 8 dogs with pulmonary embolism were used for radioimaging. Using the best modulation ratio of 2-IT and McAb SZ-51, radiolabelling yield was 90%-95% as determined by ITLC. The results of ELISA showed that immunoactivity was not affected. Radioimaging results showed that 99mTc-SZ-51 concentrated in the pulmonary embolism 1 hour after injection, and the image became clear gradully within 3 h. Quantitative analysis 3 h after injection showed that the ratios of radioactive uptake between the thrombus and surrounding lung were 5.3±0.5 in vivo imaging and 7.0±1.2 in vitro imaging. Higher uptake was found in fresh pulmonary thrombus than in old one, The ratio of them was 2.65 in vitro measurement.  
        关键词：Monoclonal antibody;Pulmonary embolism;Radioimmuoimaging;Animals laboratory   
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        发布时间：2021-03-12
      • WU Weiyi 1 FAN Wo 2 BAO Junjie 1 WU Jinchang 1 1
        Issue 11, (2002)
        摘要：A direct labelling method for anti-CEA monoclonal antibody (McAb), C 50 was labelled with 188Re has been established. Making use of sodium gluconate as chelator, SnCl 2 as reducing agent, the McAb C 50 was reduced by Vitamin C, and then labelled with 188Re. In this process, the in vitro stability of labelled 188Re-C 50 was observed, the concentration of SnCl 2, sodium gluconate, and McAb as well as the affection of interval time of adding McAb on radiochemical purity of labelled C 50 were determined. SPECT imaging was performed to show the biological distribution of 188Re labelled C 50 after injected into the tumor of nude mice. The pathologic study was observed by electron microscopy after therapy. The experimental results showed that the optimal condition for 188Re labelling C 50 was as follows: the concentration of SnCl 2, sodium gluconate and McAb was 2.2-4.0g/L, 0.3mol/L and 2.5×10 5-5.0×10 5mol/L respectively. The labelling efficiency 2h post labelling was as high as 90%. The radioactivity was localized in the tumor after 188Re-C 50 was injected into it, and the tumor began to shrink 2 weeks later, the disruption and necrosis of the tumor cells were observed microscopically.  
          
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        发布时间：2021-03-12
      • MIN Xiaofeng 1 XU Jianying 1 CUI Haiyan 1,2 ZHANG Jiong 1,2 ZHOU Wei 1,2 CHEN Minfeng 1 YIN Duanzhi 21
        Issue 11, (2002)
        摘要：188Re-HEDP was concentrated in bone after intravenous injection into the tumor- bearing rats. Biodistribution study showed that 188Re retained in bony lesions was much higher than normal bone (P<0.05). The blood clearance curve showed its pharmacodynamics of two phase models. After injection of 188Re-HEDP, most of the radioactivity was excreted by the urinary system. Within 72h the accumulative excretion rate was 82% (80% of the injected dose was found in the urine). Radiopharmaceutical study in urine excretion was mainly in the form of 188Re-HEDP. In vitro, the binding to proteins in plasma was found to be 86.4%±0.30% for 188Re-HEDP.  
        关键词：188Re-HEDP;Pharmacokinetics   
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        发布时间：2021-03-12
      • JIANG Zhong WANG Feng LIU Zengli TANG Jun WU Jinchang
        Issue 11, (2002)
        摘要：To evaluate the radiobiodistribution and internal dosimetry of 99mTc-TRODAT-1 in human target organs. Three patients with Pakinson’ s disease (PD) and two patients with essential tremor (ET) were included. After injection of 99mTc-TRODAT-1 intravenously, serial whole body scans were aquired during the following 24h by dual-head SPECT. Radioactivity uptakes were estimated applying ROI technique and radioactivity-time curves in target organs were obtained with non-linear regression analysis in terms of Microcal Origin 5.0 software. Residence time was calculated from the accumulative activity. All data of the residence times were input to the MIRDOSE 3.1 software to create the organ-absorbed dose. Brain tomography for each patient was performed 3h post injection. The experimental results showed that the internal absorbed dose from 99mTc-TRODAT-1 in target organs was small and the striatum was clearly imaged. Also, demonstrates that 99mTc-TRODAT-1 is a safe and ideal radio tracer to visualize the changes of dopamine transporter in striatum.  
        关键词：Internal absorbed dose;99mTc-TRODAT-1;Dopamine transporter/Brain   
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        发布时间：2021-03-12
      • WANG Fujun SHAO Junguo LI Xingtao LI Jinfeng LIU Yalin
        Issue 11, (2002)
        摘要：The left ventricular functions were examined by radionuclide ventriculography in 84 patients with acute viral myocarditis and 11 normal persons. The patients were divided into two groups. One group was treated with Ginseng-Ophiopogon injection. The other was with conventional therapy. The results showed that the systolic and diastolic functions of left ventricule in patients with acute viral myocarditis were damaged with various magnitude and recovered gradually after therapy. At last ,we found that the therapeutic effect of Ginseng-Ophippogon injection is significantly better than that of conventional therapy.  
        关键词：Ginseng-Ophiopogon injection;Acute viral myocarditis;Radionuclid ventriculogrophy   
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        发布时间：2021-03-12
      • YU Yongli YU Jianfang ZHU Reisen
        Issue 11, (2002)
        摘要：patients with cold thyroid nodule were imaged with 201Tl (early 15min, delayed 120min). Regions of interest (ROIs) were generated over the cold nodule and normal tissue on each image by semi-quantification method. The semi-quantitative analysis was performed by using a lesion to non-lesion ratio of early(ER) and delayed(DR) images. Retention index(RI) was calculated by dividing delayed-early subtraction to product ER. There was significant difference in the ER,DR and RI between malignant and benign lesions (P<0.05). The difference was not significant between carcinoma and inflammation (P>0.05), but there was significant difference between carcinoma and cystic adenoma (P<0.01).The sensitivity, specificity and accuracy using semi-quantification were significantly higher than visual method, and DR,RI were higher than ER (P<0.05). 201Tl imaging of thyroid cold nodule using ER,DR and RI have good clinical application value.  
        关键词：Radionuclide imaging;201Tl;Thyroid nodule   
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        发布时间：2021-03-12
      • TANG Jun LIU Ning YANG Yuanyou JIN Jiannan
        Issue 11, (2002)
        摘要：At is a promising radioisotope for cancer therapy due to its favorable radiobiological characteristics. The progress of radiotherapeutic drugs labelled with 211At is reviewed based upon 39 references. Additionally, its present status, problems and prospect are also discussed.  
        关键词：At;Labelled compound;Cancer;Radiotherapy   
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        发布时间：2021-03-12
      • YU Junfeng YIN Duanzhi WANG Yongxian
        Issue 11, (2002)
        摘要：This paper describes in details the advances in radioactive intervention therapy of liver cancer. It includes the radionuclides, agents and their respective advantages and disadvantages. Finally, the therapeutic protocols, effect and dose calculation etc. were discussed also.  
        关键词：Radioactive intervention therapy;Liver cancer;Radionuclide   
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        发布时间：2021-03-12
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