最新刊期
      卷 0 ， 期 0 ， 2020
      • Issue 5, (2000)
        摘要：The purpose of this artical is to study the preparation and biodistribution in animal of dopamine transporter imaging agent 125I- β-CIT. β-CIT was 125I radioiodinated with odogen method, the dynamic distribution of 125I-β-CIT in brain and critical organs were studied with SD rat （atuoradiography） and NIH mice respectively. The radiolabelling yield of 125I-β-CIT was 84%, the radiochemical purity was better than 98%. Blood clearance could be explained by two-compartment model with a duration of 12h, (α = 3.87, T1/2α =0.179, β =0.162, T1/2β =4.276) and three-compartment model in 24h, (Pi= 5.28, T1/2Pi= 0.131, α = 0.403, T1/2α. = 1.719, β= 0.040, T1/2β = 17.298). The maxim uptake rate of brain （9.1% ± 1.0%） was reaches at lh, while at 24h, the target/noise ratio was higher. Critical organs liver, lung, spleen and kidney had high uptake rate [（9.881 1.43）- （16.29± 1.72）], except liver, other organs showed quick clearance (T1/2<4h). Thyroid had a low uptake. Autoradiography of the brain showed that striatum uptake was good, striatum /cerebral cortex radioactivity ratio achieved maximum at 20h. The results show that 125-β-CIT has a high striatum uptake and good stability in vivo, can provide good SPECT forages, the best acquisition time of SPECT may be about 20h after i.v..  
        关键词：Dopamine transporter;Biodistribution;Autoradiography CL R817.1   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：To investigate the characteristics of 125I-VIP binding to human gastrointestinal adenocarcinoma and their adjacent tissue. VIP was labeled with Na125I using chloramine -T method, separated by Sephadex G-50 column chromatography and examined by silica 60F254 thin layer chromatography. The effects of time and temperature on the specific binding were examined, saturable binding and competitive binding between 125I-VIP and VIP receptors in membranes from gastrointestinal adenocarcinoma and their adjacent tissue were carried out. The results showed that labeling rate was 70%, the specific activity of 125I-VIP was 18TBq/mmol, the radiochemical purity was better than 98%. A single specific binding sites of high affinity were present in gastric adenocarcinoma and its adjacent tissue as well as in colon adenocarcinoma and its adjacent tissue. The Kd and Bmax values were 1.19nmol/L and 339fmol/mg protein, 1.26nmol/L and 156fmol/mg protein, 0.97nmol/L and 304fmol/mg protein, 1.53nmol/L and 151fmoll/mg protein respectively. The present study demonstrated that the binding sites of VIP on human gastrointestinal adenocarcinoma were more abundant than their adjacent tissue membrane, which provide valuable experimental evidence for VIP receptor imaging.  
        关键词：VIP receptor;Gastrointestinal adenocarcinoma;Receptor analysis   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：To investigate the effect of serotonin uptake inhibitor, clomipramine, on binding of dopamine transporter ligand 125I-β-CIT in mouse brain. Dose-dependant changes in 125I-β-CIT specific binding induced by clomipramine were studied in the striatum （rich in DAT）, frontal cortex and brain stem （rich in 5-HTT）. Changes in the time-activity curves of 125I-P-CIT specific binding after clomipramine injection were also examined. The specific binding of 125I-β-CIT in brain stem, hippocampus, frontal cortex was inhibited significantly （vs controls P<0.01）, while the binding in the striatum was greater than contyols. The binding in brain stem, frontal cortex and hippocampus were inhibited at all time points （from 5 minutes to 8h）, while the binding in the striatum were enhanced from 5min to 8h except at 6h. Enhancement of 125I-β-CIT binding in the striatum after clomipramine treatment indicates there was the possibility of interaction between serotonin and dopamine system. The uptake of 125I-β-CIT in brain is reduced by clomipramine administration and this result has not yet reported in literatures.  
        关键词：Dopamine transporter(DAT);125I-P-CIT;Serotonin transporter (5-HTT);Clomipramine   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：SPECT coincidence imaging was performed on a dual-head gamma camera （DST-XLi, SMV） to allow the detection of 511keV gamma emission of 18FDG positron radionuclides. 18FDG was provided by Shanghai Institute of Nuclear Research. All the patients with lung lesions on X-ray film have not yet confirmed in diagnosis underwent 18FDG imaging. Patients fasted 4 hours before administration of 18FDG and kept rest from 1-2 hours after intravenous administration of 37-111MBq 18FDG. Images were reconstructed by lterative reconstruction. Draw ROI on lesion area （L） and control area （C）. The L/C ratio larger than 1.3, it was considered positive. Of total 39 cases, the sensitivity, specificity and accuracy was 93%（25/27）, 83%（10/12） and was 90%（35/39） respectively, for the diagnosis of lung cancer. Therefore coincidence imaging of 18FDG-SPECT is a suitable and reliable new technology for the diagnosis of lung cancer.  
        关键词：18FDG imaging;Coincidence SPECT;Lung cancer   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：Myocardial blood perfusion （MBP） ECT and quantitative analysis were performed in 10 canines with experimental acute myocardial infarct（AMI）. The accuracy of main myocardial quantitative index, including defect volume（DV） and defect fraction（DF）, was estimated and correlated with histchemical staining （HS） of infarcted area. Other 21/AMI canines were divided into Nd:YAG laser tyansmyocardial revascularization treated group LTMR and control group. All canines were performed MBP ECT after experimental AMI. Results found that the infarcted volume （IV） measured by HS has well correlated （r=0.88） with DV estimated by myocardial quantitative analysis. But the DF values calculated by both methods was not significantly different （t= 1.28 P>0.05）. In LTMR group 27.5% ± 3.9%, the DF is smaller than control group 32.1% ± 4.6% （t = 2.49 P<0.05）. Results indicate that 99mTc-MIBI myocardial perfusion SPECT and quantitative study can accurately predict the myocardial blood flow and magnitude of injured myocardium. Nd:YAG LTMR could improve myocardial blood perfusion of ischemic myocardium and decrease effectively the infarct areas.  
        关键词：Myocardial infarction;Radionuclide myocardial imaging;Laser transmyocardial revascularization   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：Three common reducing agents: stannous chloride （SnCl2）, ascorbic acid （AA）, 2-mercapoethanol （2-ME） have been compared for their efficacy of labeling antibodies（Abs） with 99mTc, stability and immunoreactivity of labeled Abs The results showed that 2-ME was an optimum reducing agent, at a molar ratio of 1:2000 for Ab to 2-ME. A 99mTc labeling efficiency greater than 95% can be obtained, the colloid formation was less than 5%. The radioimmunoimaging of 99mTc-BAC5 in nude mice bearing tumor xenografts showed that tumor can be viewed clearly.  
        关键词：Isotope labeling/method;Antibody;Technetium-99m;Radioimmunoimaging   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：To evaluate the therapeutic effect of phosphorus-32 glass microsphere (32P-GMS) on hepatocellular carcinoma. By Seldinger’s procedure, super-selective catheterization was performed to the distal end of proper hepatic artery for instilling 32p-GMS. Ninety-nine times of interventional internal irradiation were accomplished in 79 cases of hepatocellular carcinoma. Results shows that surviving rates in A or B regimen at half year, 1, 2 and 3 years were 100%, 96.7%, 56.7% and 43.3% or 97.9%, 91.8%, 61.2% and 51.0% respectively. The median surviving time after therapy was 585 days single multifactorial analysis revealed liver function, tumor size, clinical typing and accumulation of 32P-GMS in the tumor mass and the therapeutic regime were the prognostic factors influencing the surviving rate of the patient. Conclusion:32P-GMS is a safe, effective and economic method for the therapeutic measure in treating hepatocellular carcinoma.  
        关键词：Phosphorus-32 glass microspheres;Radioactive medicine;Internal irradiation interventional therapy;Liver carcinoma   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：Results of lymphoscintigraphy in 78 patients with clinically suspected malignant lymphoma and leukemia were reported and its clinical value for in diagnosis of hematological diseases were evaluated. Confirmed by pathological examination, 30 cases were diagnosed as malignant lymphoma and 24 cases non-malignant lymphoma. In malignant lymphoma, the sensitivity of lymphoscintigraphy was 83.3% and the specificity 62.5%, where the sensitivity of CT and ultrasound, were 83.3%, 66.7% and 22.2% respectively. Confirmed by bone marrow biopsy, leukemia was found in 9 cases and non-leukemia in 15. In leukemia, the sensitivity of lymphoscintigraphy was 88.9% and specificity 53.3%. Whereas the sensitivity of CT, was 50%. Therefore, the lymphoscintigraphy have comparatively high sensitivity for the diagnosis of malignant lymphoma and leukemia.  
        关键词：Lymphoscintigraphy;99mTc-DX   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：To investigate the influence of 153Sm-EDTMP and 89SrCl2 on immune function in patients with multiple bone metastate. 86 cases of multiple bone metastases were treated with 153Sm-EDTMP and 89SrCl2 during the period of 1995-1998. The patient’s RBC, WBC, T lymphocyte subset, blood immunoglobulin and tumor necrosis factor（TNF） were also measured. Difference in the RBC, T lymphocyte subset, and immunoglobulin and TNF before and after therapy and also between 153Sm-EDTMP and 89SrCl2 group both was not significant （P>0.05）. Althrough the WBC have decreased, but still within normal range. As the dose of 153Sm-EDTMP increased, the toxicity of bone narrow rose then the WBC and RBC of patient and blood IgG decreased, however, T lymphocyte subset has not changed. Common dosage of 153sm-EDTMP and 89SrCl2 did not influence patient’s immune function significantly. However with the increasing of therapeutic dose it’s inevitable to influence their immune function and possible to effect the humonal function first.  
        关键词：Bone metastases;Radionuclide therapy;Immune function   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：Characteristics of single beam collimated by Leksell Gamma Unit have been simulated using EGS4 Monte Carlo method. Results of the simulation indicated that non-scattered photons dominated in the aperture of final collimator and the number of non-scattered photons was 2 to 3 order bigger than the number of scattered Photons. Outside the aperture, the number of scattered photon could not be ignored. In addition, Compton scatter was the major interaction of photon with the final collitnator.  
        关键词：Gamma knife;Monte Carlo simulation;Collimated system   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：To investigate the possible role of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) on the pathogenesis of Graves’ ophthalmopathy (GO), the serum IL-6 and TNF-alpha concentrations were evaluated by radioimmunoassay from 38 patients with GO, 33 patients with Graves’ disease (GD) without ophthalmopathy, and also from 24 healthy subjects. The serum IL-6, TNF-alpha levels were significantly higher in the patients with GO (x ± s 341.7 ± 151.8 and 784.4 ± 1103.5pg/mL) than those in the patients with GD (x ± s 174.7 ± 53.5 and 597.9± 400.0pg/mL), (P<0.01, P<0.05). No significant difference in the mean serum IL-6, TNF-alpha was found between the patients with GD and normal control group (P>0.05). The serum levels of IL-6 and TNF-alpha in the patients with GO were positively correlated (r=0.45, P<0.01). The results suggested that IL-6 and TNF-alpha might be an important factor in the pathogenesis of GO.  
        关键词：Cytokines;Graves’ ophthalmopathy;Graves’ disease;Radioimmunoassay   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：125I-rhirudin was prepared by chloramine-T method. The affinity of rhirudin with thrombin-fibrin complex was analyzed with solid phase saturation method and competitive method. The kinetics of the rhirudin-thromb in-fibrin complex formation was studied too. Results indicated that rhirudin was able to bind with thrombin-fibrin complex as a tenary complex, but not directly with fibrinogen. The molecular binding ratio was 14:14:1 for rhirudin:thrombin:fibrinogen. The affinity constant of rhirudin with thrombin-fibrin complex was 9.72×106L/mol, and the physiological Ca2+ concentration was their optimal binding environment. The binding reaction of rhirudin with thrombin-fibrin complex could reach the equilibrium in 30 to 45 minutes. All these results indicate that rhirudin is hopefully a new ligand for thrombotic image.  
        关键词：r-hirudin;Thrombin;Fibrinogen;Thrombus;Radioligandassay   
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        发布时间：2021-03-12
      • Issue 5, (2000)
        摘要：188Re (Eβmax= 2.11MeV; E,= 155keV; T1/2= 16.9h) is an attractive therapeutic radionuclide which is produced from decay of the reactor-produced 188W parent (T1/2= 69h) and thus conveniently obtained on demand by elution from the 188W/188Re generator system. It is very useful for remote region far from reactor. This paper reviews the physical and chemical properties, nuclear characters as well as the biological behavier in vivo and vitro of the 188Re; preparation of the 188W/188Re generator and labelling chemistry of 188Re. The recent progress of the 188Re and its labelled compounds applied in the clinical studies of cancer therapy, synovectomy and intravascular radiation therapy were also exampled. It is concluded that the application of 188W/188Re generator in the clinic therapy should be expanded as the 99Mo/99mTc generator in the clinic diagnosis.  
        关键词：188Re;188W;Therapy;Generator   
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        发布时间：2021-03-12
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