Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents

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Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents

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- Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents
- NUCLEAR TECHNIQUES Issue 5, (2000)
- 作者机构：
  
  1. 广州市红十字会医院,广州,510220
  2. 中山医科大学,广州,510089
  3. 广州市红十字会医院!广州510220中山医科大学,广州,510089
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.3
- Published：01 May 2000
- 稿件说明：
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[1]张汝森,刘长征.~(99m)Tc直接法标记抗体的3个常用还原剂的评价[J].核技术,2000(05):312-315.

Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents[J]. Nuclear techniques, 2000, (5): 312-315.
[1]张汝森,刘长征.~(99m)Tc直接法标记抗体的3个常用还原剂的评价[J].核技术,2000(05):312-315. DOI：

Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents[J]. Nuclear techniques, 2000, (5): 312-315. DOI：

摘要

比较了

９９ｍ

Ｔｃ直接法标记抗体的３个常用还原剂：氯化亚锡（ＳｎＣｌ

２

），２－巯基乙醇（２－ＭＥ）和抗坏血酸（ＡＡ）的

９９ｍ

Ｔｃ标记率、标记抗体的稳定性及免疫活性。结果显示。２－ＭＥ是一个良好的还原剂，当抗体／２－ＭＥ摩尔比为１∶２０００时，抗体的标记率可达到９５％以上，胶体形成量少于５％，

９９ｍ

Ｔｃ－ＢＡＣ

５

肿瘤放射免疫显像，鼻咽癌移植瘤影像清晰。

Abstract

Three common reducing agents: stannous chloride （SnCl

）

ascorbic acid （AA）

2-mercapoethanol （2-ME） have been compared for their efficacy of labeling antibodies（Abs） with

99m

stability and immunoreactivity of labeled Abs The results showed that 2-ME was an optimum reducing agent

at a molar ratio of 1:2000 for Ab to 2-ME. A

99m

Tc labeling efficiency greater than 95% can be obtained

the colloid formation was less than 5%. The radioimmunoimaging of

99m

Tc-BAC

in nude mice bearing tumor xenografts showed that tumor can be viewed clearly.

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Related Author

刘长征

谢瑶

邹俊涛

梁昌盛

卢汉平

陈维真

ZHANG Yong1 XU Jiehua1 CHEN Weizhen1 LU Hanping2 LIANG Changsheng3 ZOU Juntao4 XIE Yao4 LIU Changzheng2 1

许杰华

Related Institution

中山大学基础医学院人体解剖学教研室

中山大学基础医学实验教学中心

中山大学基础医学院实验核医学教研室

Department of Nuclear Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China) 2

Department of Nuclear Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630) 2

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