The study of the relationship of r-hirudin and prethrombus

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The study of the relationship of r-hirudin and prethrombus

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- The study of the relationship of r-hirudin and prethrombus
- NUCLEAR TECHNIQUES Issue 5, (2000)
- 作者机构：
  
  1. 江苏省原子医学研究所核医学国家重点实验室,无锡,214063
  2. 中国科学院生物物理研究所
- 作者简介：
- 基金信息：
  
  卫生部科研基金!96－1－344
- DOI：
  CLC： R817.4
- Published：01 May 2000
- 稿件说明：
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[1]谭成,万卫星,张荣军,张莲芬,韩玉珉,金坚.重组水蛭素与血栓前体关系的研究[J].核技术,2000(05):343-346.

The study of the relationship of r-hirudin and prethrombus[J]. Nuclear techniques, 2000, (5): 343-346.
[1]谭成,万卫星,张荣军,张莲芬,韩玉珉,金坚.重组水蛭素与血栓前体关系的研究[J].核技术,2000(05):343-346. DOI：

The study of the relationship of r-hirudin and prethrombus[J]. Nuclear techniques, 2000, (5): 343-346. DOI：

摘要

氯胺－Ｔ法碘标水蛭素，采用固相饱和法、固相竞争法分析水蛭素与凝血酶－纤维蛋白原复合物的亲和力及定量结合关系，并分析水蛭素－凝血酶－纤维蛋白原复合物形成的时间反应动力学。结果显示，水蛭素可特异地与凝血酶－纤维蛋白原复合物形成三元复合物，它们的分子配比为１４∶１４∶１（水蛭素：凝血酶：纤维蛋白原）。生理Ｃａ

２＋

浓度是它们的最佳结合环境，水蛭素与凝血酶－纤维蛋白原复合物结合的亲和常数Ｋ

ａ

为９．７２ ×１０

６

Ｌ／ｍｏｌ；其结合反应可在３０－４５ｍｉｎ达到平衡，提示水蛭素有作为血栓显像配基的可能性。

Abstract

125

I-rhirudin was prepared by chloramine-T method. The affinity of rhirudin with thrombin-fibrin complex was analyzed with solid phase saturation method and competitive method. The kinetics of the rhirudin-thromb in-fibrin complex formation was studied too. Results indicated that rhirudin was able to bind with thrombin-fibrin complex as a tenary complex

but not directly with fibrinogen. The molecular binding ratio was 14:14:1 for rhirudin:thrombin:fibrinogen. The affinity constant of rhirudin with thrombin-fibrin complex was 9.72×10

L/mol

and the physiological Ca

concentration was their optimal binding environment. The binding reaction of rhirudin with thrombin-fibrin complex could reach the equilibrium in 30 to 45 minutes. All these results indicate that rhirudin is hopefully a new ligand for thrombotic image.

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金坚

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State Key Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine

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