最新刊期
卷 0 ， 期 0 ， 2020
- 摘要：The result of radioligand binding assay study demonstrated that in the prostate both cytoplasmic and nuclear AR can be saturated by 5 nmol/1 dihydrotestosterone （DHT）. The values of Bmax and KD are 129.4 fmol/mg protein and 0.88 nmol/1 in cytoplasm; 176.2 fmol/mg protein and 3.2 nmol/1 in nucleus. Both the cytoplasmic and nuclear AR content in prostatic cancer are higher than that in normal prostate and benign prostatic hypertrophy （P<0.05）. It is useful in selecting suitable therapy for PC patients. Thaw-mount autoradiography demonstrated that AR are mainly located in the epithelial cells of the glandular tissue of prostate.
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发布时间：2021-04-09 - 摘要：The effects of sarsasapogenine （SAR）, an active principle of Anemorrhenae Rhizome （知母）on peripheral lymphocyte β-adrenoceptors （β-AR） and serum cortisol contents were studied in rabbit hydrocortison models produced by repeated injections of therapeutic doses of hydrocortisone. The lymphocyte β3-AR binding sites （SB） were measured with 125I-PIN binding assay and the serum cortisol contents were estimated with radioimmunoassay. Experimental data revealed that hydrocortisone treatment markedly elevated the lymphocyte SB （0.65±0.03 and 1.48±0.13 fmol/106 cells before and after treatment） as well as the serum cortisol （128±21 and 239±45 nmol/1 before and after treatment）. Oral administration of SAR for 6 days decreased the lymphocyte β-AR significantly （1.48±0.13 and 0.76±0.06 fmol/106 cells before and after administration）. On the contrary, SAR showed no significant effect on serum cortisol contents.. Therefore the down-regulation effect of SAR on peripherel lymphocyte β-AR, which might reflect one of the clinical effects of Anemorrhenae Rhizome for treating "Yin Deficiency"syndrome, is unlikely to be mediated through an influence on serum cortisol content.The mechanism of the down-regulation effect of SAR on peripheral lymphocyte β-AR remains to be studied.
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发布时间：2021-04-09 - 摘要：Using H-bepridil as a radioligand and calmodulin （CaM, an intracellular calcium receptor） as a receptor, the characteristics of 3H-bepridil binding to CaM from bovine brain were analyzed with equilibrium column binding technique. The 3H-bepridil radioreceptor binding assay is also established. Binding of 3H-bepridil to CaM is specific, saturable and reversible only in the presence of calcium. Scatchard analysis revealed that KD was 2.52±0.44 μmol/1 （n=5） and Bmax was 13.95±2.33 nmol/mg protein （n=5）. Hill analysis demonstrated that nH was 0.96±0.03 （n=5）. The intra-CV or inter-CV of this method was about .10% （n=22）, indicating good stability and reproducibility. The results of competitive inhibition experiments of newly synthesized compounds indicates that the affinity of all bepridil analogs to CaM was similar, with that of bepridil.
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发布时间：2021-04-09 - 摘要：The bone mineral contents（BMC） of radius and ulna of 7972 individuals of 4-90 years old living in Shanghai were measured by GMY-1 bone mineral detector, which is based on γ-ray absorptiometry technique. The results obtained showed that the BMC at 4-19 years old increased yearly, the growing rate being quicker in males than in females. The BMC of 20 to 29 years old group grew slowly and remained relatively constant at years 30-39. Then it started to fall down when the age was over 40 years old. The speed of reduction in females was twice or thrice times quicker than that in males. The BMC was obviously decreased when females were above 50 years old and males were above 60 years old.
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发布时间：2021-04-09 - 摘要：Cardiac function was measured by radionuclide cardiac blood-pool imaging in 15 normal persons, 19 cases of hypertension, 32 cases of coronary heart disease, 35 cases of coronary heart disease combined with hypertension and 44 cases of myocardial infarction. Significant differences have been found in indices of cardiac function between normal subjects and patients with coronary heart disease and coronary heart disease combined with hypertension, even though the patients were without any clinical sign of cardiac failure. Lowered regional EF and decreased ventricular wall motion were found in 38.8% of patients, while 65.7% of patients revealed marked abnormality in MFR. The results indicate that latent cardiac dysfunction is common in patients with coronary heart disease. The earliest change is diastolic function abnormalities.
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发布时间：2021-04-09 - 摘要：In this article, the plasma level of atrial natriuretic factor （ANF） in 40 patients with acute viral myocarditis and 20 healthy subjects （controls） were measured by radioimmunoassay （RIA）. The results showed that the level of ANF in patients was significantly lower than that of normal controls. The mechanism of the decrease of plasma ANF in this disease and its clinical significance were discussed.
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发布时间：2021-04-09 - 摘要：The measurement of FT3 anid FT4 is one of the most direct indicators of thyroid status. A thyronine derivative （T3X） was synthesized and used as the precursor for preparing 125I-T3X and 125I-T4X with high specific activity. The antiserum titres are 1:160000 for T3 and 1:6000 for T4,and the affinity constants （K） are 1.13×1011 I/mol for T3 and 7.6×10101/mol for T4. Using specific precipitating reagent to separate F and B, the one-step direct method of RIA has been established. It is accurate, specific and stable. The sensitivity is 0.24pmol/l for FT3 and 0.16pmol/l for FT4. The direct method and its kits have been used succes-sfully in some clinical laboratory. When compared with Amersham’s Amerlex-M kits, the results of both are quite coincidence.
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发布时间：2021-04-09 - 摘要：The serum TSH of 452 patients were measured with a two-site immunoradio-metric assay （IRMA）. The serum TSH level was 1.69±0.95μIU/ml in euthyroid subjects, and was much lower in patients with hyperthyroidism （i. e. 0.3μIU/ml） which returned to nearly normal in 85% patients who were in remission stage. On the contrary, serum TSH level elavated to more than 5μIU/ml in patients with hypothyroidism. TRH stimulation test showed that the baseline serum TSH values correlated well with the peak serum TSH value post-TRH （r=0.88）. TSH （IRMA） improved the sensitivity over that of conventional radioimmunoassays, hence has made possible discrimination between hyperthyroid, euthyroid and hypothyroid subjects, and may serve as a first-line test in the diagnosis of thyroid dysfunction. In addition, when serum thyroid hormone levels have been brought to normal range, a further "fine adjustment" of treatment based on serum TSH levels appears to be helpful to achieve physiologically and biochemically.
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发布时间：2021-04-09 - 摘要：In order to explore the excellent condition for the preparation of 99mTc-MIBI, the kinetics of preparation reaction was studied using MIBI freeze-dried kit. The result obtained has shown that the preparation reaction exhibits a first-order reaction. The relationship between temperature and rate constant （k）can be expressed by the equation: lnk= -13502.3/ T+36.8（ r=0.9907）. The activation energy Ea is equal to 0.11MJ/mol. In order to make.the labelling yield of 99mTc-MIBI higher than 95%, the reaction time should be longer than 3 min at 100℃.
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发布时间：2021-04-09 - 摘要：The 125I-iodopindolol was prepared with the lodogen or Chloramine-T method. The result suggested that iodination of pindolol with lodogen was more simple and had a higher iodinating rate. The binding of 125I-iodopindolol to membrane protein of rat lung tissue was specific, saturable and reversible.关键词：125I-iodopindolol;lodogen;Chloramine-T;Beta-adrenoceptor
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发布时间：2021-04-09 - 摘要：Analogue dementia modelof rats was produced by electrolytic lesion of brain. α1 -adrenergic receptors were assayed by radioligand binding assay （RRBA）. It was found that Bmax of α1 receptors was decreased obviously in cerebral certex, hippo- campus and cerebellum of analogue dementia rats and was raised obviously by Heart-and Kidney- Benefiting Chinese Herbs as well as by Hydergin.The electrolytic lesion did not change the activity of MAO-B in model rat brains. Neither Heart- and Kidney- Benefiting Chinese Herb Medicines nor Hydergin showed marked effect on brain MAO-B activity of the model rats.
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发布时间：2021-04-09 - 摘要：Seven cases of extremely severe acute radiation injuries treated with fetal liver cells transplantation （FLT） are reported in this article. Patients received 6-8 Gy whole body irradiation which was followed by an infusion of fetal liver cell suspension. Hematological reconstitution occurred in all patients and temporary chimera developed in 3 patients after FLT.There were no difference between the hematologic reconstitution in patients with or without chimera.
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发布时间：2021-04-09 - 摘要：The binding capacities of estrogen receptors in nuclear matrix, nuclei and cytosol of human breast cancer tissue （EmR, EnR, EcR） were estimated with radioligand binding assay of receptors.The average Bmax values of these components in 21 breast cancer specimens are 417.54±170.95,147.75±98.32, 7.34±5.33 fmol/mg protein, andthose in 10 normal breast tissue specimens are 42.33±8.49, 25.05±7.81, 5.91±2.28 fmol/mg protein. Comparing the cancer and normal breast tissues, there is significant difference in Bmax values of EmR and EnR（P<0.01）, and no difference in Bmax values of EcR （P>0.10）. The EmR/EnR value of 21 breast cancer tissue is 0.65±0.10, and that of 10 normal breast tissue is 0.42±0.04. There is statistical difference between the cancer and normal. 10 of 13（77%） patients, who are EcR-positive, have higher EmR/EnR values （≥0.50）. The results suggest that estrogen receptors are mainly located at the nuclear matrix, ER levels in nucleus, especially in nuclear matrix of breast cancer tissue are valuable parameters and may be useful for predicting whetner the patient will be responsible to endocrine therapy.
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发布时间：2021-04-09 - 摘要：50 cases of epilepsy have been examined by SPECT with 99mTc-ECD. All of these were performed during the internal period. Interval scan showed decreased uptake in the region of seizure focus. Most seizure foci were found in temporal and frontal lobe. Compared with X-CT and EEG, SPECT gave higher percentage of positive results, and may be helpful for clinical treatment.
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发布时间：2021-04-09
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