Study on the methodology of FT<sub>3</sub> and FT<sub>4</sub> radioimmunoassay （direct method）

Xu Shuxiang Zou Songhai Yang Wenru

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Study on the methodology of FT₃ and FT₄ radioimmunoassay （direct method）

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- Study on the methodology of FT₃ and FT₄ radioimmunoassay （direct method）
- NUCLEAR TECHNIQUES Issue 5, (1992)
- 作者机构：
  
  1. 上海南洋医学放射免疫检测中心
  2. 上海铁道医学院附属甘泉医院
  3. 中国科学院上海原子核研究所,上海,200020
  4. ,上海,201800
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：01 May 1992
- 稿件说明：
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[1]许叔祥,邹颂海,杨文如,唐国忠,赵夏令,周美英,殷林祥.FT_3、FT_4放射免疫分析(直接法)方法学研究[J].核技术,1992(05):285-290.

Xu Shuxiang Zou Songhai Yang Wenru. Study on the methodology of FT₃ and FT₄ radioimmunoassay （direct method）[J]. Nuclear techniques, 1992, (5): 285-290.
[1]许叔祥,邹颂海,杨文如,唐国忠,赵夏令,周美英,殷林祥.FT_3、FT_4放射免疫分析(直接法)方法学研究[J].核技术,1992(05):285-290. DOI：

Xu Shuxiang Zou Songhai Yang Wenru. Study on the methodology of FT₃ and FT₄ radioimmunoassay （direct method）[J]. Nuclear techniques, 1992, (5): 285-290. DOI：

摘要

自行研制了

125

I标记的T

X、T

X（酰胺类衍生物）。免疫所得抗T

抗体滴度1:160000

K=1.13×10

l/mol;抗T

抗体滴度1:6000

K=7.6×10

l/mol。以PR试剂为分离剂

建立一步直接放免测定法。灵敏度FT

为0.24 pmol/l;FT

为0.16pmol/l。方法精确、特异、稳定。临床应用结果与英国Amersham Amerlex—M法相关极好。

Abstract

The measurement of FT3 anid FT4 is one of the most direct indicators of thyroid status. A thyronine derivative （T3X） was synthesized and used as the precursor for preparing 125I-T3X and 125I-T4X with high specific activity. The antiserum titres are 1:160000 for T3 and 1:6000 for T4

and the affinity constants （K） are 1.13×1011 I/mol for T3 and 7.6×10101/mol for T4. Using specific precipitating reagent to separate F and B

the one-step direct method of RIA has been established. It is accurate

specific and stable. The sensitivity is 0.24pmol/l for FT3 and 0.16pmol/l for FT4. The direct method and its kits have been used succes-sfully in some clinical laboratory. When compared with Amersham’s Amerlex-M kits

the results of both are quite coincidence.

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Study on the methodology of FT3 and FT4 radioimmunoassay （direct method）

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摘要

Abstract

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references

Study on the methodology of FT₃ and FT₄ radioimmunoassay （direct method）