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1. 中国科学院高能物理研究所核分析技术开放实验室
2. 中国科学院微生物研究所
3. 中国科学院高能物理研究所核分析技术开放实验室,北京,100039
4. ,北京,100080
纸质出版日期:2003-07-01
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[1]刘静,章佩群,张博润,钱琴芳,陈春英,柴之芳,丁文军.利用中子活化分析法研究高生物量富铬酵母的培养条件[J].核技术,2003(07):509-513.
LIU Jing1 ZHANG Peiqun1 ZHANG Borun2 QIAN Qinfang1 CHEN Chunying1 CHAI Zhifang1 DING Wenjun1 1. Neutron activation study of cultivation conditions for high biomass chromium-rich yeast[J]. Nuclear techniques, 2003, (7): 509-513.
[1]刘静,章佩群,张博润,钱琴芳,陈春英,柴之芳,丁文军.利用中子活化分析法研究高生物量富铬酵母的培养条件[J].核技术,2003(07):509-513. DOI:
LIU Jing1 ZHANG Peiqun1 ZHANG Borun2 QIAN Qinfang1 CHEN Chunying1 CHAI Zhifang1 DING Wenjun1 1. Neutron activation study of cultivation conditions for high biomass chromium-rich yeast[J]. Nuclear techniques, 2003, (7): 509-513. DOI:
利用中子活化分析法对富铬酵母中的铬进行了测定。以啤酒酵母菌株LZ-53为出发菌株对培养基的种类、糖度、铬浓度、pH值、装液量、接种量和培养时间等培养条件进行了优化
确定了最佳培养条件为:麦芽汁作培养基
培养基中糖浓度为10Brix
铬浓度为1200mg/mL
pH6.0
装液量为8%
接种量为10%
培养时间为30h。在此条件下干菌体铬含量可达3248mg/g。
The instrumental neutron activation analysis was used to determine Cr content in chromium-rich yeast cells. Through a series of tests
the optimized cultivation parameters of Saccharomyces cerevisiae LZ-53 were obtained as follows: wort as medium
10Brix sugar concentration; Cr 1200mg/mL in wort; the initial pH6.0; 8% culture medium; inoculum volume 10% (V/V)
and yeast cells harvested in 30 h. Under the optimized cultivation conditions
the chromium content of strain LZ-53 reached 3248mg/g.
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