氧化石墨烯纳米片对淀粉样蛋白纤维的影响

鄢朋; 霍庚阳; 储新星; 张公军; 李宾; 周星飞

doi:10.11889/j.0253-3219.2019.hjs.42.090501

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氧化石墨烯纳米片对淀粉样蛋白纤维的影响

核物理、交叉学科研究 | 更新时间：2024-10-25

- 氧化石墨烯纳米片对淀粉样蛋白纤维的影响
  封面论文
- The effect of graphene oxide nanosheets on the dissociation of amyloid fibers
- 核技术 2019年42卷第9期文章编号：090501
- 作者机构：
  
  1.宁波大学物理系宁波 315211
  2.中国科学院宁波材料技术与工程材料所宁波 315211
  3.中国科学院上海应用物理研究所上海 201800
- 作者简介：
  
  鄢朋，男，1991年出生，2015年毕业于井冈山大学，现为硕士研究生，研究领域为淀粉样蛋白对神经退行性疾病影响
  周星飞，E-mail：zhouxingfei@nbu.edu.cn
- 基金信息：
  
  国家自然科学基金(11474173;31670871);浙江省自然科学基金(LY18A040003);宁波市自然基金(2018A610220)
- DOI：10.11889/j.0253-3219.2019.hjs.42.090501
  中图分类号： TL99
- 收稿日期：2019-05-29，
  
  修回日期：2019-07-03，
  
  纸质出版日期：2019-09-10
- 稿件说明：
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鄢朋,霍庚阳,储新星等.氧化石墨烯纳米片对淀粉样蛋白纤维的影响[J].核技术,2019,42(09):47-52.

YAN Peng,HUO Gengyang,CHU Xinxing,et al.The effect of graphene oxide nanosheets on the dissociation of amyloid fibers[J].NUCLEAR TECHNIQUES,2019,42(09):47-52.
鄢朋,霍庚阳,储新星等.氧化石墨烯纳米片对淀粉样蛋白纤维的影响[J].核技术,2019,42(09):47-52. DOI： 10.11889/j.0253-3219.2019.hjs.42.090501.

YAN Peng,HUO Gengyang,CHU Xinxing,et al.The effect of graphene oxide nanosheets on the dissociation of amyloid fibers[J].NUCLEAR TECHNIQUES,2019,42(09):47-52. DOI： 10.11889/j.0253-3219.2019.hjs.42.090501.

摘要

淀粉样蛋白纤维是引发神经退行性疾病的重要原因，抑制和阻碍蛋白纤维的形成已成为预防相关疾病的重要手段。为了快速、高效地清除、降解成熟胰岛素纤维以治疗II型糖尿病，本文用Hummer法制备了平均厚度为1 nm的氧化石墨烯纳米片（Graphene Oxide Nanosheets，GOs），研究了GOs对胰岛素纤维的作用。实验结果显示：GOs能够快速地解离成熟胰岛素纤维并清除，其解离效果随GOs浓度的提高而显著加强。结合ThT荧光和显微傅里叶红外变换光谱(Micro Fourier Transform Infrared Spectroscopy

Micro-FTIR)的测量结果推测GOs是通过抽取胰岛素纤维的蛋白分子改变纤维的二级结构，将其从原来的β折叠结构转变为α螺旋结构从而达到解离作用。

Abstract

Background

The formation of amyloid fibers is closely related to the neurodegenerative diseases. Inhibiting the formation of mature amyloid fibers has become an important approach to prevent related diseases.

Purpose

This work aims to prepare an efficient and rapid material to remove and degrade mature insulin fibers for the treatment of type II diabetes mellitus.

Methods

In this study

the effect of graphene oxide nanosheets (GOs) with an average thickness of 1 nm on mature insulin fibers was prepared by Hummer method. Both the ThT fluorescence and Micro Fourier Transform Infrared Spectroscopy (Micro-FTIR) were employed to measure the outputs.

Results

GOs can effectively dissociate mature insulin fibers into short fibers and remove them. The dissociation effect is significantly enhanced with the increase of GOs concentration.

Conclusions

Combining the results of ThT fluorescence and Micro-FTIR

speculate that GOs changes the second structures of the mature insulin fibers by extracting the protein molecules

transforming it from the original beta fold to an alpha spiral structure.

关键词

Keywords

references

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