培养基相态对菌膜结构和机械性能的调控

刘诗马; 吕敏; 罗立强; 郭竞颺; 王丽华; 樊春海

doi:10.11889/j.0253-3219.2018.hjs.41.110504

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培养基相态对菌膜结构和机械性能的调控

核物理、交叉学科研究 | 更新时间：2021-05-19

- 培养基相态对菌膜结构和机械性能的调控
- Regulation of the structure and mechanical properties of biofilm by medium phase
- 核技术 2018年41卷第11期文章编号：110504
- 作者机构：
  
  1.上海大学理学院上海 200444
  2.中国科学院上海应用物理研究所上海 201800
- 作者简介：
  
  [ "刘诗马, 男, 1989年出生, 2013年毕业于河南师范大学, 现为博士研究生, 研究领域为新型抗菌纳米材料", "LIU Shima, male, born in 1989, graduated from Henan Normal University in 2013, doctoral student, focusing on new antibacterial nanomaterials" ]
  吕敏, E-mail:lvmin@sinap.ac.cnLYU Min, E-mail:lvmin@sinap.ac.cn
  罗立强, E-mail:luck@shu.edu.cnLUO Liqiang, E-mail:luck@shu.edu.cn
- 基金信息：
  
  国家重点研发项目(2016YFA0201200);国家重点研发计划(2016YFA0400900);口腔疾病研究国家重点实验室开放课题(SKLOD2018OF01);国家自然科学基金(61571278);国家自然科学基金(U1432116)
- DOI：10.11889/j.0253-3219.2018.hjs.41.110504
  中图分类号： TL99
- 收稿日期：2018-07-16，
  
  修回日期：2018-09-26，
  
  纸质出版日期：2018-11-10
- 稿件说明：
移动端阅览
刘诗马, 吕敏, 罗立强, 等. 培养基相态对菌膜结构和机械性能的调控[J]. 核技术, 2018,41(11):110504

Shima LIU, Min LYU, Liqiang LUO, et al. Regulation of the structure and mechanical properties of biofilm by medium phase[J]. Nuclear techniques, 2018, 41(11): 110504
刘诗马, 吕敏, 罗立强, 等. 培养基相态对菌膜结构和机械性能的调控[J]. 核技术, 2018,41(11):110504 DOI： 10.11889/j.0253-3219.2018.hjs.41.110504.

Shima LIU, Min LYU, Liqiang LUO, et al. Regulation of the structure and mechanical properties of biofilm by medium phase[J]. Nuclear techniques, 2018, 41(11): 110504 DOI： 10.11889/j.0253-3219.2018.hjs.41.110504.

摘要

菌膜（Biofilm）是细菌为了适应不利的生存环境，粘附于基底形成的、由细菌和其分泌的胞外基质（Extracellular Polymeric Substance，EPS）组成的三维类凝胶复合物。具有类凝胶结构的菌膜，已被用于工业污水处理、生物工程和生物电池等领域。然而，如何简单而有效地调控菌膜结构使其更有利于应用依然是一个难题。本研究选取革兰氏阳性菌（枯草芽孢杆菌）和阴性菌（绿脓杆菌）作为模式生物，通过流变学测试和扫描电子显微成像技术研究不同相态（固态和液态）培养基对两种细菌菌膜结构的影响。结果显示：菌膜结构和类凝胶性质依赖于培养基的相态；与液态培养基相比，固态培养基培养细菌形成的菌膜结构松散、但厚度较厚且机械强度更强。本研究为调控菌膜结构和机械性能提供了一种简便的方法，为菌膜在有机污染物吸附和新型生物材料构建方面提供了新思路。

Abstract

Background

Bacteria tends to form biofilm to adapt to the unfavorable living environment. Biofilm

a three-dimensional hydrogel-like compound

is composed of bacteria adhering to the substrate and its extracellular polymeric substance (EPS). Biofilm with gel-like structure has been widely used in industrial wastewater treatment

bioengineering and bio-battery.

Purpose

This study aims to simply and effectively regulate biofilm for practical applications.

Methods

Gram-positive bacteria (

Bacillus subtilis

) and -negative bacteria (

Pseudomonas aeruginosa

) were selected as model organisms to investigate the effect of different phase media (solid and liquid) on biofilm structure by rheological tests and scanning electron microscopy.

Results

The results showed the gel-like structure of biofilm depended on the medium phase. Compared to the liquid medium

biofilm cultured with solid medium possessed loose

thicker and stronger mechanical strength.

Conclusions

This study provides a simple method for regulating the structure and mechanical properties of biofilm

and also provides insight into the application of biofilm for adsorption of organic pollutants and the design of new biomaterials.

关键词

Keywords

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