电离辐射诱导小鼠骨髓树突状免疫细胞急性凋亡的研究

张红; 罗冰芝; 徐凤兰; 胡晓娅; 张哲; 刘小琴

doi:10.11889/j.0253-3219.2018.hjs.41.030301

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电离辐射诱导小鼠骨髓树突状免疫细胞急性凋亡的研究

核化学、放射化学、放射性药物和核医学 | 更新时间：2021-05-19

- 电离辐射诱导小鼠骨髓树突状免疫细胞急性凋亡的研究
- Acute apoptosis of mice bone marrow dendritic cells induced by ionizing irradiation
- 核技术 2018年41卷第3期文章编号：030301
- 作者机构：
  
  1.甘肃省武威肿瘤医院武威 733000
  2.中国科学院近代物理研究所兰州 730000
- 作者简介：
  
  [ "张红, 女, 1959年出生, 1996年于兰州大学获理学博士学位, 研究领域为生物物理学", "ZHANG Hong, female, born in 1959, graduated from Lanzhou University with a doctoral degree in 1996, focusing on biophysics" ]
- 基金信息：
  
  国家自然科学基金(11605260)
- DOI：10.11889/j.0253-3219.2018.hjs.41.030301
  中图分类号： R594.8
- 收稿日期：2017-12-04，
  
  修回日期：2018-01-17，
  
  纸质出版日期：2018-03-10
- 稿件说明：
移动端阅览
张红, 罗冰芝, 徐凤兰, 等. 电离辐射诱导小鼠骨髓树突状免疫细胞急性凋亡的研究[J]. 核技术, 2018,41(3):030301

Hong ZHANG, Bingzhi LUO, Fenglan XU, et al. Acute apoptosis of mice bone marrow dendritic cells induced by ionizing irradiation[J]. Nuclear techniques, 2018, 41(3): 030301
张红, 罗冰芝, 徐凤兰, 等. 电离辐射诱导小鼠骨髓树突状免疫细胞急性凋亡的研究[J]. 核技术, 2018,41(3):030301 DOI： 10.11889/j.0253-3219.2018.hjs.41.030301.

Hong ZHANG, Bingzhi LUO, Fenglan XU, et al. Acute apoptosis of mice bone marrow dendritic cells induced by ionizing irradiation[J]. Nuclear techniques, 2018, 41(3): 030301 DOI： 10.11889/j.0253-3219.2018.hjs.41.030301.

摘要

观察X射线辐射对小鼠骨髓树突状免疫细胞（Dendritic Cells，DCs）的影响。利用X射线对小鼠骨髓树突状免疫细胞进行不同剂量的照射，流式细胞术检测小鼠骨髓DCs的凋亡比例、CCK-8方法检测DCs免疫能力的变化情况，利用蛋白抑制剂对JAK2/STAT3信号转导通路进行抑制，探讨可能的分子机理。与未照射组比较，X射线照射0.5 Gy能够诱导小鼠骨髓DCs抗原递呈和抑制肿瘤细胞活性能力下降，凋亡比例增加，JAK2、STAT和Caspase-3蛋白表达显著增加。X射线能够诱导小鼠骨髓DCs凋亡增加，从而导致细胞免疫能力下降，JAK2/STAT3/Caspase-3通路可能是参与X射线诱导DCs凋亡的机制之一。

Abstract

Background

Radiation would induce apoptosis of cells.

Purpose

This study aims to observe the effect of irradiation on bone marrow dendritic cells (BMDCs) in mice.

Methods

The BMDCs were irradiated by X-ray to absorbed doses of 0.5 Gy or 1.0 Gy. The apoptosis and immunity ability of DCs were measured by flow cytometry and CCK-8. Protein expressions were determined by Western blot. The JAK2/STAT3 signal transduction pathway was inhibited by protein inhibitors.

Results

The dysfunction of BMDCs induced by X-ray irradiation was observed. The BMDCs apoptosis percentage increased

and JAK-2

STAT-3

and cleaved Caspase-3 protein expressions were promoted.

Conclusion

The up-regulation of JAK2

STAT3 and cleaved Caspase-3 expression induced by X-ray irradiation results in apoptosis and dysfunction of BMDCs

and may represent a new mechanism in radiation-induced immune suppression.

关键词

Keywords

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