藻蓝蛋白对辐射致小鼠氧化损伤的保护作用

刘琪; 李文军; 陆丽娜; 谢园园; 王明超; 秦松; 杜振宁

doi:10.11889/j.0253-3219.2018.hjs.41.010302

您当前的位置：

首页 >

文章列表页 >

藻蓝蛋白对辐射致小鼠氧化损伤的保护作用

核化学、放射化学、放射性药物和核医学 | 更新时间：2021-05-19

- 藻蓝蛋白对辐射致小鼠氧化损伤的保护作用
- Protective effect of phycocyanin against oxidative damage induced by radiation
- 核技术 2018年41卷第1期文章编号：010302
- 作者机构：
  
  1.烟台大学药学院烟台 264005
  2.中国科学院烟台海岸带研究所烟台 264003
  3.兰州大学核科学与技术学院兰州 730000
- 作者简介：
  
  [ "刘琪, 女, 1991年出生, 2015年毕业于滨州医学院, 现为硕士研究生, 药学专业", "LIU Qi, female, born in 1991, graduated from Binzhou Medical University in 2015, master student, major in pharmacy" ]
  [ "李文军, 男, 1984年出生, 2017年毕业于中国科学院大学, 现为助理研究员, 海洋生物学专业", "LI Wenjun, male, born in 1984, graduated from University of Chinese Academy of Sciences in 2017, assistant researcher, major in marine biology" ]
  秦松, E-mail:sqin@yic.an.cn DU Zhenning, E-mail:sqin@yic.an.cn
  杜振宁, E-mail:zhenning.du@163.com QIN Song, E-mail: zhenning.du@163.com
- 基金信息：
  
  中国科学院战略性先导科技专项(XDA1102040300);海洋公益性行业科研专项(20120527);国家自然科学基金(41176144);国家自然科学基金(41376139);国家科技支撑计划(2013BAB01B01);烟台市科技计划(2016JHZB007);海洋经济创新发展区域示范专项海洋药源生物种质资源库建设项目(12PYY001SF08-ZKYHADS-1)
- DOI：10.11889/j.0253-3219.2018.hjs.41.010302
  中图分类号： TL7;R963
- 收稿日期：2017-06-28，
  
  修回日期：2017-09-07，
  
  纸质出版日期：2018-01-10
- 稿件说明：
移动端阅览
刘琪, 李文军, 陆丽娜, 等. 藻蓝蛋白对辐射致小鼠氧化损伤的保护作用[J]. 核技术, 2018,41(1):010302

Qi LIU, Wenjun LI, Lina LU, et al. Protective effect of phycocyanin against oxidative damage induced by radiation[J]. Nuclear techniques, 2018, 41(1): 010302
刘琪, 李文军, 陆丽娜, 等. 藻蓝蛋白对辐射致小鼠氧化损伤的保护作用[J]. 核技术, 2018,41(1):010302 DOI： 10.11889/j.0253-3219.2018.hjs.41.010302.

Qi LIU, Wenjun LI, Lina LU, et al. Protective effect of phycocyanin against oxidative damage induced by radiation[J]. Nuclear techniques, 2018, 41(1): 010302 DOI： 10.11889/j.0253-3219.2018.hjs.41.010302.

摘要

通过建立X射线辐射损伤小鼠模型，研究藻蓝蛋白（Phycocyanin，PC）对辐射引起鼠氧化损伤的保护作用。选取C57 BL/6雄性小鼠72只，按体重随机分为正常对照组、单纯辐射组、PC预防组、阳性对照组，每组18只。辐射前连续灌胃相应受试样品7 d，在第8 d，除正常组外，其余各组接受全身一次性6 Gy的X射线照射。各组分别在辐射后的1 d、3 d、7 d留取血浆和组织，并检测血浆及肝组织中的抗氧化酶活性及肝组织中活性氧自由基（Reactive Oxygen Species，ROS）含量。实验结果表明，藻蓝蛋白显著提高了辐射后小鼠血浆中和肝脏中抗氧化酶超氧化物歧化酶（Superoxide Dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione Peroxidase，GSH-PX）的活性（

0.05），降低了丙二醛（Malondialdehyde，MDA）的含量（

0.05），减少肝组织中ROS的含量（

0.05）。结果表明，藻蓝蛋白可提高小鼠抗氧化能力，减轻辐射对机体造成的氧化损伤，对小鼠辐射损伤有较好的防护作用。

Abstract

Background

Phycocyanin (PC) is a pigment protein present in cyanobacteria

cryptophyta

etc

Purpose

This study aims at the protective effect of phycocyanin on oxidative damage induced by irradiation by establishing injury mouse model by X-ray irradiation.

Methods

72 C57 BL/6 male mice were randomly divided into normal control group

irradiation model group

PC pretreatment group and positive control group. The mice were continuously fed for 7 d. On the 8th day of intragastric administration

except for the normal control group

the rest of the mice were subjected to total body irradiation to a dose of 6 Gy for the preparation of radiation damage model. The plasma and tissues were collected at 1 d

3 d and 7 d after irradiation

and the activities of antioxidant enzymes in plasma and liver were measured. The content of reactive oxygen species (ROS) in liver tissue was detected by dihydroethidium (DHE) staining.

Results

Phycocyanin increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in the plasma of mice after irradiation

and increased the activity of SOD (

0.05)

decreased malondialdehyde (MDA) content (

0.05)

and reduced liver tissue ROS content (

0.05).

Conclusion

The results show that phycocyanin can improve the antioxidant capacity of mice

reduce oxidative damage to the body caused by irradiation

and provide a better protective effect on irradiation injury of mice.

关键词

Keywords

references

G D C Toro , P Toro , L M Zieher , . Sensitivity of cerebellar glutathione system to neonatal ionizing radiation exposure . Neurotoxicology , 2007 . 28 ( 3 ): 555 - 561 . DOI: 10.1016/j.neuro.2006.12.002 http://doi.org/10.1016/j.neuro.2006.12.002 .

D Thotala , S Chetyrkin , B Hudson , . Pyridoxamine protects intestinal epithelium from ionizing radiation-induced apoptosis . Free Radical Biology and Medicine , 2009 . 47 ( 6 ): 779 - 785 . DOI: 10.1016/j.freeradbiomed.2009.06.020 http://doi.org/10.1016/j.freeradbiomed.2009.06.020 .

宋璐非 , 刘冰 , 赵勇 , . 天然藻蓝蛋白对SD大鼠的长期毒性研究 . 中国医药导报 , 2012 . 9 ( 33 ): 15 - 17 . DOI: 10.3969/j.issn.1673-7210.2012.33.005 http://doi.org/10.3969/j.issn.1673-7210.2012.33.005 .

Lufei SONG , Bing LIU , Yong ZHAO , . Chronic toxicity study of phycocyanin on Sprague Dawley rats . China Medical Herald , 2012 . 9 ( 33 ): 15 - 17 . DOI: 10.3969/j.issn.1673-7210.2012.33.005 http://doi.org/10.3969/j.issn.1673-7210.2012.33.005 .

B Fernández-Rojas , J Hernández-Juárez , J Pedraza-Chaverri . Nutraceutical properties of phycocyanin . Journal of Functional Foods , 2014 . 11 375 - 392 . DOI: 10.1016/j.jff.2014.10.011 http://doi.org/10.1016/j.jff.2014.10.011 .

H Mansour . Protective role of carnitine ester against radiation-induced oxidative stress in rats . Pharmacological Research , 2006 . 54 ( 3 ): 165 - 171 . DOI: 10.1016/j.phrs.2006.04.003 http://doi.org/10.1016/j.phrs.2006.04.003 .

李程程 , 张恒 , 吴红英 , . 白藜芦醇对小鼠辐射氧化损伤的保护性研究 . 陕西医学杂志 , 2013 . 42 ( 5 ): 515 - 517 . http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_sxyxzz201305001 .

Chengcheng LI , Heng ZHANG , Hongying WU , . The protective effects of resveratrol on irradiation induced oxidative damage in mice . Shaanxi Medical Journal , 2013 . 42 ( 5 ): 515 - 517 . http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_sxyxzz201305001 .

李园园 , 张宇睿 , 徐文清 . 一氧化氮清除剂Fe(DETC) 2 对小鼠的辐射防护作用 . 辐射研究与辐射工艺学报 , 2017 . 35 ( 4 ): 040203 DOI: 10.11889/j.1000-3436.2017.rrj.35.040203 http://doi.org/10.11889/j.1000-3436.2017.rrj.35.040203 .

Yuanyuan LI , Yurui ZHANG , Wenqing XU . Radioprotective effects of a nitric oxide scavenger, Fe(DETC) 2 , on mice . Journal of Radiation Research and Radiation Processing , 2017 . 35 ( 4 ): 040203 DOI: 10.11889/j.1000-3436.2017.rrj.35.040203 http://doi.org/10.11889/j.1000-3436.2017.rrj.35.040203 .

J Zheng , T Inoguchi , S Sasaki , . Phycocyanin and phycocyanobilin from Spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress . AJP: Regulatory, Integrative and Comparative Physiology , 2013 . 304 ( 2 ): R110 - R120 . DOI: 10.1152/ajpregu.00648.2011 http://doi.org/10.1152/ajpregu.00648.2011 .

D Xia , B Liu , X Luan , . Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice . Chinese Journal of Oceanology and Limnology , 2016 . 34 ( 2 ): 399 - 404 . DOI: 10.1007/s00343-015-4312-6 http://doi.org/10.1007/s00343-015-4312-6 .

姚星辰 , 段雅彬 , 王财 , . 牦牛活性蛋白对X射线损伤小鼠的防护作用 . 营养学报 , 2015 . 37 ( 5 ): 442 - 447 . DOI: 10.13325/j.cnki.acta.nutr.sin.2015.05.012 http://doi.org/10.13325/j.cnki.acta.nutr.sin.2015.05.012 .

Xingchen YAO , Yabin DUAN , Cai WANG , . Protective effects of YAK activated protein on X-ray injury in mice . Acta Nutrimenta Sinica , 2015 . 37 ( 5 ): 442 - 447 . DOI: 10.13325/j.cnki.acta.nutr.sin.2015.05.012 http://doi.org/10.13325/j.cnki.acta.nutr.sin.2015.05.012 .

陈洁 , 张永芹 , 毕洁静 , . 耐辐射球菌PprI蛋白对急性放射损伤小鼠防护作用的初步研究 . 辐射研究与辐射工艺学报 , 2012 . 30 ( 5 ): 286 - 290 . http://d.wanfangdata.com.cn/Thesis/Y2120237 .

Jie CHEN , Yongqin ZHANG , Jiejing BI , . Studying the protective effect of deinococcus radiodurans PprI on acute radiation injury in mice . Journal of Radiation Research and Radiation Processing , 2012 . 30 ( 5 ): 286 - 290 . http://d.wanfangdata.com.cn/Thesis/Y2120237 .

S Rong , Y Zhao , W Bao , . Curcumin prevents chronic alcohol-induced liver disease involving decreasing ROS generation and enhancing antioxidative capacity . Phytomedicine , 2012 . 19 ( 6 ): 545 - 550 . DOI: 10.1016/j.phymed.2011.12.006 http://doi.org/10.1016/j.phymed.2011.12.006 .

E Selzer , A Hebar . Basic principles of molecular effects of irradiation . Wiener Medizinische Wochenschrift , 2012 . 162 ( 3-4 ): 47 - 54 . DOI: 10.1007/s10354-012-0052-9 http://doi.org/10.1007/s10354-012-0052-9 .

Hideyuki Tominaga , Seiji Kodama , Matsuda Naoki , . Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation . Journal of Radiation Research , 2004 . 45 ( 2 ): 181 - 188 . DOI: 10.1269/jrr.45.181 http://doi.org/10.1269/jrr.45.181 .

李英哲 , 黄连珍 , 周丽玲 . 维生素A缺乏对大鼠脂质过氧化和抗氧化系统的影响 . 营养学报 , 2001 . 23 ( 1 ): 1 - 5 . DOI: 10.13325/j.cnki.acta.nutr.sin.2001.01.001 http://doi.org/10.13325/j.cnki.acta.nutr.sin.2001.01.001 .

Yingzhe LI , Lianzhen HUANG , Liling ZHOU . Effect of vitamin a deficiency on lipid peroxidation and antioxidation system in rats . Acta Nutrimenta Sinica , 2001 . 23 ( 1 ): 1 - 5 . DOI: 10.13325/j.cnki.acta.nutr.sin.2001.01.001 http://doi.org/10.13325/j.cnki.acta.nutr.sin.2001.01.001 .

T Finkel , N J Holbrook . Oxidants, oxidative stress and the biology of ageing . Nature , 2000 . 408 239 - 247 . DOI: 10.1038/35041687 http://doi.org/10.1038/35041687 .

许靖逸 , 李祥龙 , 李解 , . 雅安藏茶茶褐素对 60 Co γ辐射损伤的防护作用 . 核技术 , 2017 . 40 ( 4 ): 040301 DOI: 10.11889/j.0253-3219.2017.hjs.40.040301 http://doi.org/10.11889/j.0253-3219.2017.hjs.40.040301 http://www.j.sinap.ac.cn/hejishu/CN/abstract/abstract735.shtml .

Jingyi XU , Xianglong LI , jie LI , . Protective effect of extracted theabrownines from Ya'an Tibetan tea on radiation damage in mice caused by 60 Co γ-ray . Nuclear Techniques , 2017 . 40 ( 4 ): 040301 DOI: 10.11889/j.0253-3219.2017.hjs.40.040301 http://doi.org/10.11889/j.0253-3219.2017.hjs.40.040301 http://www.j.sinap.ac.cn/hejishu/CN/abstract/abstract735.shtml .

Kim Hyeong-Geug , Jang Seong-Soon , Lee Jin-Seok , . Panax ginseng Meyer prevents radiation-induced liver injury via modulation of oxidative stress and apoptosis . Journal of Ginseng Research , 2017 . 41 159 - 168 . DOI: 10.1016/j.jgr.2016.02.006 http://doi.org/10.1016/j.jgr.2016.02.006 .

X Xue , X Han , Y Li , . Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis . Stem Cell Research & Therapy , 2017 . 8 ( 1 ): 7 DOI: 10.1186/s13287-016-0464-3 http://doi.org/10.1186/s13287-016-0464-3 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

雅安藏茶茶褐素对⁶⁰Co γ辐射损伤的防护作用