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1.上海交通大学 上海 200240
2.上海市计量技术测试研究院 上海 201203
[ "颜未, 男, 1991年出生, 2013年毕业于南华大学, 现为硕士研究生, 主要从事核能科学研究", "YAN Wei, male, born in 1991, graduated from University of South China in 2013, master student, focusing on nuclear and technology" ]
蒯琳萍, E-mail:lkuai@stju.edu.cn KUAI Linping, E-mail:lkuai@stju.edu.cn
收稿日期:2017-06-25,
修回日期:2017-08-22,
纸质出版日期:2017-12-10
移动端阅览
颜未, 唐方东, 何林锋, 等. γ辐射对青海弧菌Q67发光强度的影响[J]. 核技术, 2017,40(12):120502
Wei YAN, Fangdong TANG, Linfeng HE, et al. Effect of gamma ray radiation on the luminous intensity of Vibrio Qinghaiensis sp. Q67[J]. Nuclear techniques, 2017, 40(12): 120502
颜未, 唐方东, 何林锋, 等. γ辐射对青海弧菌Q67发光强度的影响[J]. 核技术, 2017,40(12):120502 DOI: 10.11889/j.0253-3219.2017.hjs.40.120502.
Wei YAN, Fangdong TANG, Linfeng HE, et al. Effect of gamma ray radiation on the luminous intensity of Vibrio Qinghaiensis sp. Q67[J]. Nuclear techniques, 2017, 40(12): 120502 DOI: 10.11889/j.0253-3219.2017.hjs.40.120502.
采用
60
Co γ射线源对青海弧菌Q67辐照,研究了辐射剂量(率)对其发光强度的影响。实验表明,在0.05 Gy·min
-1
、0.1 Gy·min
-1
、0.2 Gy·min
-1
三种剂量率下,青海弧菌Q67经过短时间的照射后,其发光强度明显受到抑制。剂量率(
d
)和照射时间(
t
)两个变量中,
d
的增加对于青海弧菌Q67发光强度影响更大,并且通过软件SPSS19.0进行回归拟合分析,发现青海弧菌Q67的相对发光值(
R
LV
)与
d
和
t
的自然对数值呈现二元线性关系。最后,以0.2 Gy·min
-1
的剂量率梯度为对象,测定了辐照后青海弧菌Q67光密度(OD
600
)的变化,发现经过不同短时间辐照后相对光密度(
R
OD
)也受到抑制,说明辐照引起的青海弧菌Q67菌体的损伤和死亡是其发光强度抑制的重要原因。研究表明,青海弧菌Q67对于γ辐照有较好的敏感性,可以作为一种潜在的评价γ射线对生命体危害的生物材料。
Background
2
The suppression of bacterial luminescence by poisonous substance has being previously reported
such as heavy metals
pesticides
however
rarely radiation.
Purpose
2
This study aims to investigate the toxicity of the bioluminescent bacteria
Vibrio Qinghaiensis sp. Q67
to γ-ray radiation and the possibility of using bacteria as a biological radiation dosemeter.
Methods
2
In this paper
the effects of γ-ray radiation on luminous intensity of Vibrio Qinghaiensis sp. Q67 are studied using
60
Co γ radiation source with various dose rate (
d
) and irradiation time (
t
).
Results
2
Experimental results show that the relative luminous values (
R
LV
) of Vibrio Qinghaiensis sp. Q67 decrease with the increase of dose rate (
d
) and irradiation time (
t
) for acute radiation
and linear bivariate associations is found between
R
LV
and the logarithm value of
d
and
t
.
Conclusion
2
Vibrio Qinghaiensis sp. Q67 is sensitive to acute γ-ray radiation
which could be used to monitor γ-ray radiation.
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