同步辐射串行晶体学静电纺丝上样实验技术

陈建樵; 汪启胜; 李冰; 周欢; 崔莹; 孙波; 王志军; 何建华

doi:10.11889/j.0253-3219.2017.hjs.40.080102

您当前的位置：

首页 >

文章列表页 >

同步辐射串行晶体学静电纺丝上样实验技术

同步辐射技术及应用 | 更新时间：2021-05-19

- 同步辐射串行晶体学静电纺丝上样实验技术
- Electrospinning sample delivery for serial crystallography using synchrotron radiation
- 核技术 2017年40卷第8期文章编号：080102
- 作者机构：
  
  1.中国科学院上海应用物理研究所张江园区上海 201204
  2.中国科学院大学北京 100049
- 作者简介：
  
  [ "陈建樵, 男, 1992年出生, 2014年毕业于四川大学, 现为硕士研究生, 从事同步辐射晶体学相关研究", "CHEN Jianqiao, male, born in 1992, graduated from Sichuan University in 2014, master student, focusing on synchrotron crystallography" ]
  何建华, E-mail:hejianhua@sinap.ac.cnHE Jianhua, E-mail:hejianhua@sinap.ac.cn
- 基金信息：
  
  中国科学院青年创新促进会(2015213);国家自然科学基金(U1632126);中国科学院战略性先导科技专项（B类）(XDB08030101)
- DOI：10.11889/j.0253-3219.2017.hjs.40.080102
  中图分类号： TL99
- 收稿日期：2017-04-11，
  
  修回日期：2017-04-28，
  
  纸质出版日期：2017-08-10
- 稿件说明：
移动端阅览
陈建樵, 汪启胜, 李冰, 等. 同步辐射串行晶体学静电纺丝上样实验技术[J]. 核技术, 2017,40(8):080102

Jianqiao CHEN, Qisheng WANG, Bing LI, et al. Electrospinning sample delivery for serial crystallography using synchrotron radiation[J]. Nuclear techniques, 2017, 40(8): 080102
陈建樵, 汪启胜, 李冰, 等. 同步辐射串行晶体学静电纺丝上样实验技术[J]. 核技术, 2017,40(8):080102 DOI： 10.11889/j.0253-3219.2017.hjs.40.080102.

Jianqiao CHEN, Qisheng WANG, Bing LI, et al. Electrospinning sample delivery for serial crystallography using synchrotron radiation[J]. Nuclear techniques, 2017, 40(8): 080102 DOI： 10.11889/j.0253-3219.2017.hjs.40.080102.

摘要

串行晶体学作为一种解析蛋白质晶体结构的新方法，因其拥有室温采集、辐射损伤低、时间分辨等优势而得到迅速发展。早期发展于自由电子激光的串行飞秒晶体学已成功实现了蛋白质微小晶体结构的解析。为了引入串行晶体学的技术优势，充分发挥第三代同步辐射的丰富资源和特点，基于上海光源BL17U1生物大分子线站，我们对串行晶体学上样方法进行了理论和实验研究，解决了静电纺丝上样方式在同步辐射上应用的难题，用溶菌酶微小晶体论证了其可行性；同时探讨了基于同步辐射的串行晶体学在毫秒时间尺度上的发展潜力，为光束线技术升级提供参考。

Abstract

Background

As a novel approach to solve the protein structure

serial femtosecond crystallography (SFX) has been developed rapidly owing to the advantages of the room-temperature data collection

lower radiation damage and time-resolved study. The first X-ray free-electron laser (XFEL) based SFX experiment has offered a new method to structure determination of protein microcrystals successfully.

Purpose

The aim is to introduce SFX into the widely-available third-generation synchrotron radiation (SR).

Methods

The electrospinning sample delivery system is a potential method to be applied on SR. The theoretical and experimental researches on electrospinning were studied using BL17U1 beamline in Shanghai synchrotron radiation facility (SSRF).

Results

A microjet containing microcrystals was produced from the liquid meniscus and controlled by an electrostatic field. The air ionization problem disturbing electrospinning in SR experiment was solved by providing vacuum condition and coaxial electrospinning technique. Using lysozyme microcrystals as a model sample

the feasibility of electrospinning was demonstrated.

Conclusion

It is shown that the serial crystallography on millisecond time scale is suitable for SR

which provides a reference for the future beamline upgrading.

关键词

Keywords

references

F Stellato , D Oberthur , M N Liang , . Room-temperature macromolecular serial crystallography using synchrotron radiation . International Union of Crystallography Journal , 2014 . 1 ( 6 ): 204 - 212 . DOI: 10.1107/S2052252514010070 http://doi.org/10.1107/S2052252514010070 .

H N Chapman , P Fromme , A Barty , . Femtosecond X-ray protein nanocrystallography . Nature , 2011 . 470 ( 7332 ): 73 - 77 . DOI: 10.1038/nature09750 http://doi.org/10.1038/nature09750 .

D P Deponte , U Weierstall , K Schmidt , . Gas dynamic virtual nozzle for generation of microscopic droplet streams . Journal of Physics D:Applied Physics , 2008 . 41 ( 19 ): 195505 DOI: 10.1088/0022-3727/41/19/195505 http://doi.org/10.1088/0022-3727/41/19/195505 .

U Weierstall , D James , C Wang , . Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography . Nature Communications , 2014 . 5 3309 DOI: 10.1038/ncomms4309 http://doi.org/10.1038/ncomms4309 .

R G Sierra , H Laksmono , J Kern , . Nanoflow electrospinning serial femtosecond crystallography . Acta Crystallographica Section D:Biological Crystallography , 2012 . 68 ( 11 ): 1584 - 1587 . DOI: 10.1107/S0907444912038152 http://doi.org/10.1107/S0907444912038152 .

M Heymann , A Opathalage , J L Wierman , . Room-temperature serial crystallography using a kinetically optimaized microfluidic device for protein crystallization and on-chip X-ray diffraction . International Union of Crystallography Journal , 2014 . 1 ( 5 ): 349 - 360 . DOI: 10.1107/s2052252514016960 http://doi.org/10.1107/s2052252514016960 .

L M G Chavas , L Gumprecht , H N Chapman . Possibilities for serial femtosecond crystallography sample delivery at future light sources . Structural Dynamics , 2015 . 2 ( 4 ): 041709 DOI: 10.1063/1.4921220 http://doi.org/10.1063/1.4921220 .

P Nogly , D James , D Wang , . Lipidic cubic phase serial millisecond crystallography using synchrotron radiation . International Union of Crystallography Journal , 2015 . 2 ( 2 ): 168 - 176 . DOI: 10.1107/S2052252514026487 http://doi.org/10.1107/S2052252514026487 .

A M Ganan-calvo , J M Montanero . Revision of capillary cone-jet physics:electrospray and flow focusing . Physical Review E , 2009 . 79 ( 6 ): 066305 DOI: 10.1103/PhysRevE.79.066305 http://doi.org/10.1103/PhysRevE.79.066305 .

Y M Shin , M M Hohman , M P Brenner , . Experimental characterization of electrospinning:the electrically forced jet and instabilities . Polymer , 2001 . 42 ( 25 ): 9955 - 9967 . DOI: 10.1016/S0032-3861(01)00540-7 http://doi.org/10.1016/S0032-3861(01)00540-7 .

O B Zeldin , M Gerstel , E F Garman . RADDOSE-3D:time-and space-resolved modelling of dose in macromolecular crystallography . Journal of Applied Crystallography , 2013 . 46 ( 4 ): 1225 - 1230 . DOI: 10.1107/S0021889813011461 http://doi.org/10.1107/S0021889813011461 .

Q S Wang , F Yu , S Huang , . The macromolecular crystallography beamline of SSRF . Nuclear Science and Techniques , 2015 . 26 ( 1 ): 010102 DOI: 10.13538/j.1001-8042/nst.26.010102 http://doi.org/10.13538/j.1001-8042/nst.26.010102 .

R G Sierra , C Gati , H Laksmono , . Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem Ⅱ . Nature Methods , 2015 . 13 ( 1 ): 59 - 62 . DOI: 10.1038/nmeth.3667 http://doi.org/10.1038/nmeth.3667 .

B K Ku , S S Kim . Electrohydrodynamic spraying characteristics of glycerol solutions in vacuum . Journal of Electrostatics , 2003 . 57 ( 2 ): 109 - 128 . DOI: 10.1016/S0304-3886(02)00106-7 http://doi.org/10.1016/S0304-3886(02)00106-7 .

J C Falkner , A M Al-somali , J A Jamison , . Generation of size-controlled, submicrometer protein crystals . Chemistry of Materials , 2005 . 17 ( 10 ): 2679 - 2686 . DOI: 10.1021/cm047924w http://doi.org/10.1021/cm047924w .

T A White , R A Kirian , A V Martin , . CrystFEL:a software suite for snapshot serial crystallography . Journal of Applied Crystallography , 2012 . 45 ( 2 ): 335 - 341 . DOI: 10.1107/S0021889812002312 http://doi.org/10.1107/S0021889812002312 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

极紫外掩模的同步辐射在波长高分辨成像仿真研究

原位同步辐射技术在超级电容器中的应用进展

钙钛矿氧化物氧八面体旋转导致的超晶格半指数峰的模拟分析

上海光源BL02U2线站掠入射X射线衍射仪的研制

20 Hz高时空分辨X射线单色光动态显微CT研究