基于催化聚合的同步辐射X射线成像标签

诸颖; 孔华庭; 张继超; 王丽华; 胡钧; 樊春海

doi:10.11889/j.0253-3219.2017.hjs.40.060103

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基于催化聚合的同步辐射X射线成像标签

同步辐射技术及应用 | 更新时间：2021-05-19

- 基于催化聚合的同步辐射X射线成像标签
- Catalytic polymerization-based X-ray imaging tags
- 核技术 2017年40卷第6期文章编号：060103
- 作者机构：
  
  中国科学院上海应用物理研究所物理生物学研究室中国科学院微观界面物理与探测重点实验室上海同步辐射光源生物成像中心嘉定园区上海 201800
- 作者简介：
  
  [ "诸颖, 女, 1981年出生, 2008年于中国科学院上海应用物理研究所获博士学位, 研究员, 研究方向为同步X射线显微成像", "ZHU Ying, female, born in 1981, graduated from Shanghai Institute of Applied Physics, Chinese Academy of Sciences with a doctoral degree in 2008, professor, focusing on synchrotron-based X-ray microscopy" ]
  樊春海, E-mail:fchh@sinap.ac.cn FAN Chunhai, E-mail:fchh@sinap.ac.cn
- 基金信息：
  
  上海市青年科技英才扬帆计划(17YF1423600);国家重点研发计划(2016YFA0400902);国家自然科学基金(11675251);国家自然科学基金(21390414);中国科学院青年创新促进会(2016236)
- DOI：10.11889/j.0253-3219.2017.hjs.40.060103
  中图分类号： TL99;O63
- 收稿日期：2017-04-13，
  
  修回日期：2017-04-21，
  
  纸质出版日期：2017-06-10
- 稿件说明：
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诸颖, 孔华庭, 张继超, 等. 基于催化聚合的同步辐射X射线成像标签[J]. 核技术, 2017,40(6):060103

Ying ZHU, Huating KONG, Jichao ZHANG, et al. Catalytic polymerization-based X-ray imaging tags[J]. Nuclear techniques, 2017, 40(6): 060103
诸颖, 孔华庭, 张继超, 等. 基于催化聚合的同步辐射X射线成像标签[J]. 核技术, 2017,40(6):060103 DOI： 10.11889/j.0253-3219.2017.hjs.40.060103.

Ying ZHU, Huating KONG, Jichao ZHANG, et al. Catalytic polymerization-based X-ray imaging tags[J]. Nuclear techniques, 2017, 40(6): 060103 DOI： 10.11889/j.0253-3219.2017.hjs.40.060103.

摘要

同步辐射X射线显微成像具有极高的空间分辨率、很好的穿透深度和优秀的能量分辨，因而在纳米分辨细胞成像领域具有巨大的应用潜力。然而，目前X射线显微技术多用于细胞结构成像，而和该技术相适合的特异性识别细胞内重要生物靶标的分子探针仍较缺乏。本工作利用同步辐射X射线良好的能量分辨特点，经体外化学催化反应成功制备了同步X射线可见的成像标签，成像分辨率达到30 nm。研究结果为进一步制备X射线敏感的分子探针，实现对细胞内生物分子的特异性识别和成像打下了良好的基础。

Abstract

Background

With short wavelengths

long penetration depth and excellent energy resolution of X-rays

synchrotron-based X-ray microscopy (XRM) has demonstrated its great potential for nanoscale cellular imaging. However

despite of the high-quality sub-cellular morphological details provided by XRM

it lacks appropriate molecular probes capable of localizing cellular targets.

Purpose

This study aims to perform the preparation of X-ray imaging tags.

Methods

In vitro

chemical catalytic reaction was introduced to get the X-ray imaging tags.

Results

By exploiting the excellent energy resolution of X-rays

synchrotron-based X-ray imaging tags were prepared with the imaging resolution of 30 nm when observed by soft X-ray microscopy at Shanghai synchrotron radiation facility (SSRF).

Conclusion

The results indicate high potential to design X-ray sensitive molecular probes for cell imaging.

关键词

Keywords

references

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