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一种生物X射线小角散射光束线站自动换样溶液蠕动装置
An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline
- 核技术 2016年39卷第1期 文章编号：010102
- 作者机构：
1.中国科学院上海应用物理研究所 张江园区 上海 201204;
2.中国科学院大学 北京 100049
- 作者简介：
洪春霞,女,1984年出生,2009年于南京航空航天大学获硕士学位,研究方向为小角散射实验站控制系统
边风刚,E-mail:bianfenggang@sinap.ac.cn
- 基金信息：
DOI：10.11889/j.0253-3219.2016.hjs.39.010102
中图分类号： TP273+.5收稿日期：2015-10-26，
录用日期：2015-12-29，
纸质出版日期：2016-01-25
- 稿件说明：
移动端阅览
洪春霞, 周平, 李怡雯, 等. 一种生物X射线小角散射光束线站自动换样溶液蠕动装置[J]. 核技术, 2016,39(1):010102
Chunxia HONG, Ping ZHOU, Yiwen LI, et al. An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline[J]. Nuclear techniques, 2016, 39(1): 010102
洪春霞, 周平, 李怡雯, 等. 一种生物X射线小角散射光束线站自动换样溶液蠕动装置[J]. 核技术, 2016,39(1):010102 DOI： 10.11889/j.0253-3219.2016.hjs.39.010102.
Chunxia HONG, Ping ZHOU, Yiwen LI, et al. An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline[J]. Nuclear techniques, 2016, 39(1): 010102 DOI： 10.11889/j.0253-3219.2016.hjs.39.010102.
摘要
利用同步辐射X射线小角散射技术可以研究溶液中蛋白质的结构信息。但是在实验过程中
高通量的X射线易造成蛋白质的辐射损伤
发生结构变化。本文介绍了一种自动换样溶液样品蠕动装置
在实验过程中利用Hamilton的PSD/4注射泵控制样品上下运动
减小单位体积照射时间以降低X射线对蛋白质的辐射损伤。此外
通过对注射泵、准直调节台和样品/缓冲液支撑台的协调控制实现了自动换样、回样和清洗功能
提高了实验效率。在上海光源生物X射线小角散射实验站进行了实验
通过对静止模式和蠕动模式下溶菌酶的散射曲线及回旋半径的测量
表明该装置可达到很好的防辐射损伤效果
实现了预期的样品蠕动装置功能。
Abstract
Background:
2
Protein structure in solution can be studied by small-angle X-ray scattering techniques at synchrotron radiation facilities. However
the structure of protein is prone to vary as the radiation damage induced by the intense X-ray beam during the experiments.
Purpose:
2
This study aims to develop an automatic solution-sample- changing peristaltic device for biological small angle X-ray scattering experiment.
Methods:
2
The injection pump PSD/4 of Hamilton is applied to eliminate the radiation damage of protein by minimizing the X-ray irradiation time of unit volume. In addition
automatic sample-changing
retrieving and cleaning was realized by the coordinated control of PSD/4
the alignment control stage and the sample/buffer control stage. Thus the experiment efficiency is improved.
Results:
2
The scattering curves and gyration radius of lysozyme under stationary collection mode and peristaltic collection mode were measured by experiments carried out at biological small angle X-ray scattering beamline of Shanghai Synchrotron Radiation Facility (SSRF). And results show that this device can prevent radiation damage of sample efficiently.
Conclusion:
2
The designed functionalities have been achieved as expected.
关键词
Keywords
references
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