硅油对淀粉样蛋白和多肽积聚的促进作用

李 佳; 雷豪志; 代 彬; 其其格; 张 益; 胡 钧

doi:10.11889/j.0253-3219.2015.hjs.38.080501

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硅油对淀粉样蛋白和多肽积聚的促进作用

核物理、交叉学科研究 | 更新时间：2021-03-03

- 硅油对淀粉样蛋白和多肽积聚的促进作用
- Silicone oil promotes the aggregation of amyloid proteins and peptides
- 核技术 2015年38卷第8期文章编号：080501
- 作者机构：
  
  1. 中国科学院上海应用物理研究所微观界面物理与探测重点实验室嘉定园区,上海,201800
  2. 中国科学院生物与化学交叉中心,上海,200032
- 作者简介：
- 基金信息：
  
  国家重大科学研究计划(No.2013CB932801)、国家自然科学基金(No.11274334)、中国科学院重点部署项目(No.KJZD-EW-M03)资助()
- DOI：10.11889/j.0253-3219.2015.hjs.38.080501
  中图分类号：
- 纸质出版日期：2015-08-13
- 稿件说明：
移动端阅览
李佳, 雷豪志, 代彬, 等. 硅油对淀粉样蛋白和多肽积聚的促进作用[J]. 核技术, 2015,38(8):080501

LI Jia, LEI Haozhi, DAI Bin, et al. Silicone oil promotes the aggregation of amyloid proteins and peptides[J]. Nuclear techniques, 2015, 38(8): 080501
李佳, 雷豪志, 代彬, 等. 硅油对淀粉样蛋白和多肽积聚的促进作用[J]. 核技术, 2015,38(8):080501 DOI： 10.11889/j.0253-3219.2015.hjs.38.080501.

LI Jia, LEI Haozhi, DAI Bin, et al. Silicone oil promotes the aggregation of amyloid proteins and peptides[J]. Nuclear techniques, 2015, 38(8): 080501 DOI： 10.11889/j.0253-3219.2015.hjs.38.080501.

摘要

本文研究了硅油对淀粉样蛋白和多肽积聚的影响，采用硫磺素T(ThT)荧光分析法和原子力显微镜表征。结果显示，5种淀粉样蛋白/多肽在含有硅油的体系中积聚更快、更充分，表明一定量的硅油能促进这些淀粉样蛋白/多肽的积聚。此外，研究了涡旋操作对硅油污染的引入。结果表明，涡旋能将离心管内壁的硅油引入溶液中，从而促进GAV9肽的积聚。

Abstract

Background: The aggregation of amyloid proteins leads to some serious diseases

such as Alzheimer’s disease

Parkinson’s disease; silicone oil is identified as biosafety material

and is widely used in industry and daily life

even implantation in vivo. Purpose: The aim is to study the influence of silicone oil on the aggregation of amyloid proteins and peptides. Methods: We measured the fluorescence of thioflavin T(ThT) in the samples and image the aggregated fibers by atomic force microscope (AFM). Results: Five kinds of amyloid proteins/peptides aggregated faster and more sufficiently in the samples containing silicone oil

and the fibers are longer and denser in silicone oil sample. Conclusion: A certain amount of silicone oil can promote the aggregation of these amyloid proteins and peptides. Abstract

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