Background: A noninvasive

real-time far-field optical microscopy is needed to study the dynamic function inside cells and proteins.However

the resolution limit of traditional optical microscope is about 200 nm due to the diffraction limit of light.So

it’s hard to directly observe the subcellular structures.Over the past several years of microscopy development

the diffraction limit of fluorescence microscopy has been overcome and its resolution limit is about tens of nanometers.Methods: To overcome the diffraction limit of light

many super-resolution fluoresce microcopies

including stimulated emission of depletion microscopy(STED)

photoactivation localization microscopy(PALM) and stochastic optical reconstruction microscopy(STORM)

have been developed.Conclusions: These methods have been applied in cell biology

microbiology and neurobiology

and the technology of super-resolution provides a new insight into the life science.