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江苏省农业科学院原子能利用研究所
纸质出版日期:1994-02-01
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[1]李正魁,张伯森.辐射共聚制备固定化酒精酵母细胞的研究[J].核技术,1994(02):119-124.
A study on immobilized ethanol yeast cells by radiation technique[J]. Nuclear techniques, 1994, (2).
在低温(-78℃)条件下,用60Coγ射线对单体丙烯酸羟乙酯(HEA)与聚乙二醇二甲基丙烯酸酯系列单体14G、9G、4G、2G等进行辐射引发共聚,获得了亲水性共聚合凝胶载体,采用这些载体吸附固定化酒精酵母细胞的乙醇产率均高于游离细胞,其中Poly(HEA—14G)共聚物载体乙醇产率是游离细胞的2.45倍,12次分批反应均高于游离细胞。实验结果表明:固定化酵母细胞的乙醇生产率与共聚体系内亲水性单体及其它单体的浓度配比、双官能团单体的链长、共聚物载体的水合度以及载体结构、多孔性因素等有关,乙醇生产率的高低取决于载体的膨胀能力和多孔性.
Hydrophilic monomer 2-hydroxyethyl acrylate(HEA) and a series of polyethylene glycol dimethacrylate monomers were copolymerised by radiation technique at low temperature(-78℃) and hydrophilic were obtained.The immobilization of yeast cells with these copolymeried carriers led to a higher ethanol productivity than free cells.Of all copolymer carriers
the ethanol yield with poly(HEA-14G) was the highest
about 2.45 times as high as that of free yeast cells.In addition
the ethanol productivity of 12 batch repeated reactions with poly(HEA-14G) carrier was all higher than that of free yeast cells.The ethanol productivity of immobilized yeast cells was dependent on the proportion of hydrophilic monomer to other monomers in copolymer systems
the chain length of the bifunctional monomer
the degree of hydration of copolymer carriers
the structure of copolymer carriers and porosity in the internal structure of carriers.The ethanol yield of immobilized cells depended on swelling ability and porosity of copolymer carriers.
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