99mTc-EHIDA radionuclide hepatobiliary imaging was performed to evaluate the hepatobiliary functional changes one to two weeks after cryosurgery in porta hepatis of porcine. The imaging shown that the radioactivity of cryosurgical hepatic region was decreased or absent. The time of appearence of radioactivity in intestines was significantly prolonged

and was statistically significant as compared with control group. The binary tract system was damaged. It was suggested that the prefect protection of binary system should be accomplished in the cryosurgery of porta hepatis to avoid severe complications.