激素、蛋白质类物质的<sup>125</sup>I标记率及标记产品的脱碘率测定

沈维明; 朱本兴; 范我

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激素、蛋白质类物质的¹²⁵I标记率及标记产品的脱碘率测定

更新时间：2021-04-09

- 激素、蛋白质类物质的¹²⁵I标记率及标记产品的脱碘率测定
- Measurement of labelling rate and deiodated rate for some hormones and proteins labelled with 125 I
- 核技术 1997年第4期
- 作者机构：
  
  苏州医学院
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： Q57
- 纸质出版日期：1997-04-01
- 稿件说明：
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[1]沈维明,朱本兴,范我.激素、蛋白质类物质的~(125)I标记率及标记产品的脱碘率测定[J].核技术,1997(04):51-55.

Shen Weiming Zhu Bening Fan Wo. Measurement of labelling rate and deiodated rate for some hormones and proteins labelled with 125 I[J]. Nuclear techniques, 1997, (4).
[1]沈维明,朱本兴,范我.激素、蛋白质类物质的~(125)I标记率及标记产品的脱碘率测定[J].核技术,1997(04):51-55. DOI：

Shen Weiming Zhu Bening Fan Wo. Measurement of labelling rate and deiodated rate for some hormones and proteins labelled with 125 I[J]. Nuclear techniques, 1997, (4). DOI：

摘要

采用氯胺－Ｔ法对胰岛素、牛血清白蛋白和猪超氧化物歧化酶进行１２５Ｉ标记，标记率采用淋洗分离时标记产品峰计数率与诸峰计数率之和的比值、三氯醋酸（ＴＣＡ）沉淀、纸层析及纸电泳等四种方法进行测定。测定结果表明，第一种方法结果偏低，与后三种方法有显著差异，而后三种方法之间无显著差异，其中尤以ＴＣＡ沉淀法操作简单、方便、速度快。还采用ＴＣＡ沉淀法对上述几种１２５Ｉ标记产品的保存条件作了探讨，结果提示，标记产品加蛋白保护，并且在低温条件下或以冻干品形式存放时脱碘率最低。

Abstract

Chloramine-T was used for 125 I labelling of insulin

bovine serum albumin and pig superoxide dismutase.The labelling rates were measured by four methods

i.e.comparing the counts from the labelled compound with those from the eluate

TCA precipitation

paper chromatograph and paper electrophoresis.It was shown that the labelling rate obtained with the first method was the lowest but those with other three methods weres imilar.Especially the TCA precipitation was considered to be the best because of its easiness and rapidit.In addition

the deiodated rates of the labelled compounds in various conditions were measured by TCA precipitation

and the lowest deiodated rate was observed for the products with some protein protection and stored at the temperature lower than -20℃ or as a lophilized state.

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激素、蛋白质类物质的125I标记率及标记产品的脱碘率测定

Measurement of labelling rate and deiodated rate for some hormones and proteins labelled with 125 I

DOI：

摘要

Abstract

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Keywords

references

激素、蛋白质类物质的¹²⁵I标记率及标记产品的脱碘率测定