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1. 中山大学
2. 中山大学昆虫学研究所
3. 中山大学中山大学昆虫学研究所
纸质出版日期:1995-07-01
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[1]张纯祥,罗达玲,李冕丰,刘小伟,曾蓉,王珣章.斜纹夜蛾核型多角体病毒DNA链断裂的辐射剂量响应[J].核技术,1995(07):405-409.
Radiation dose response of Strand breaks in SINPV-DNA[J]. Nuclear techniques, 1995, (7).
用琼脂糖凝胶电泳法测定了斜纹夜蛾核型多角体病毒(SINPV)经60Coγ射线辐照产生的DNA单链和双链断裂频数,得到在1-100kGy剂量范围内,DNA单、双链断裂频数与吸收剂量的关系。实验结果表明,SINPV-DNA对γ辐射的链断裂响应可以用单靶一次击中和二次击中复合模型描述,单链断裂是一次击中效应,而病毒DNA的双链断裂是一次击中和二次击中事件的共同效应,其中以二次击中事件效应为主。
The Spodoplera litura Nuclear Polyhedrosis Viruses (SINPV)is a kind of insectile virus with a simple structure
in which a double helix DNA is encapsulated in a protein coat and there is no function of enzymatic repair. In this study
the SINPV samples in dry powdered form held in sealed plastic tube were irradiated by 1-100kGy gamma rays. The single strand breaks (SSB) and double strand breaks (DSB) induced in SINPV after irradiation were measured by neutral and alkaline agarose gel eletrophoresis.A dose-response function combining the responses of one-hit and two-hit events was used to describe the SSB and DSB dose-response curves. It is shown that the SSB are one-hit events and the DSB are the combination of both one-hit
and two-hit events
and two-hit events are predominant in the DSB process.
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