MAO Shuhong1, 2 JIN Genming1 WEI Zengquan1 XIE Hongmei1 GU Ying3 MA Qiufeng1 1. Screening of respiration deficiency mutants of yeasts (Saccharomyces cerevisiae) induced by ion irradiation and the mtDNA restriction analysis[J]. Nuclear techniques, 2005, (11).
MAO Shuhong1, 2 JIN Genming1 WEI Zengquan1 XIE Hongmei1 GU Ying3 MA Qiufeng1 1. Screening of respiration deficiency mutants of yeasts (Saccharomyces cerevisiae) induced by ion irradiation and the mtDNA restriction analysis[J]. Nuclear techniques, 2005, (11).DOI:
Screening of the respiration deficiency mutants of Saccharomyces cerevisiae induced by 5.19 MeV/u 22Ne5 + ion irradiation is reported in this paper. Some respiration deficiency mutants of white colony phenotype
in a condition of selective culture of TTC medium
were obtained. A new and simplified method based on mtDNA restric- tion analysis is described. We found that there are many obvious differences in mtDNAs between wild yeasts and the respiration deficiency mutants. The mechanism of obtaining the respiration deficiency mutants induced by heavy ion irradiation is briefly discussed.