A method of observing the blood supply of rabbits with replanted limb by Na99TcmO4 trace imaging ( TTI ) is described and compared with clinical routine method( CRM ) . 40 healthy adult rabbits with replanted limb were divided into and A ( n = 25) and B ( n = 15 ) groups by 20 min after operation. Locally freezing ( < 5°C ) 10 min was given to group A and locally warming ( 40—45°C ) was given to group B. Group C ( 5 normal control rabbits ) was kept at common temperature ( 20 — 25°C ) . Dynamic and static images were performed on all rabbits. The radioac- tivity uptake of replanted limb/ normal limb ratio ( T/NT ) was analyzed using the region of interest analysis. The vascular crisis ( VC ) in 45 rabbits was evaluated with explorative operation. The Ο

ⅠandⅡscales were classified according to their radionuclide distribution and the T/NT were 0.142 ± 0.061

0.472 ± 0.055

0.843 ± 0.184 ( t = 7.23

13.65

2.71

P < 0.01 )

respectively. The Ο scales ( T/NT < 0.25 ) of TTI can be regarded as a semi-quantitative and qualitative standard on diagnosing VC. 25

3

0 cases in groups A

B and C was confirmed with VC by explorative operation. The success rate of making VC model by locally freezing was 100%. The sensitivity( 96.4% ) in diag- nosing VC by TTI was much higher than CRM ( 39.3% ) ( χ 2 = 20.9

P < 0.01 ) . It is conclude that the blood supply of rabbits with replanted limb could be reported more accurately and directly by TTI than CRM. TTI could play an important role on early and no traumatic diagnosing VC in patients with replanted finger or limb.