The preparation of [99mTc(CO)3(OH2)3]+ using carbonyl kits and the labeling of morpholinos (MORF)

a DNA analogue

were described. A 25 mer MORF with a primary amine on the 3’ equivalent end attached via a 10 member linker was conjugated with DTPA. The carbonyl kits were reconstituted according to the manufacturer’s recommendation. The reversed phase and size exclusion HPLC indicate that the Tc-carbonyl intermediate’s yield was more than 90%. The DTPA conjugated MORF was successfully radiolabeled

the labeling efficiency ranged (63±7)%

and the specific activity

which was dependent on the specific activity of [99mTc(CO)3(OH2)3]+

was (9.62 ±1.26) GBq/μg. In saline at room temperature and in fresh human serum at 37°C

the radioactivity profile was unchanged over 48 h. This strategy for preparing [99mTc(CO)3(OH2)3]+ using carbonyl kits and labeling MORF with [99mTc(CO)3(OH2)3]+ therefore appears to be suitable for further applications.