Report gene HSV1-TK and therapy gene were connected by IRES

and recombinant adenovirus vector Ad5-TK-IRES-BDNF-EGFP was constructed and infected with BMSCs at MOI of 0

50

100

150

200 and 250

with the control recombinant adenovirus vector of Ad5-EGFP. Green fluorescence cell positive rate was observed under the microscopy. MTT assay was used to determine the cell proliferation. bFGF and EGF were used to induce the BMSCs

and RQ-PCR to determine target gene expression in infection BMSCs. Uptake of 131I-FIAU was assessed by gamma counter. The data were processed by SPSS11.code. Recombinant adenovirus at MOI 150 had high infection efficiency and low toxic in BMSCs. There was a strong relation between the mRNA expression of TK and BDNF in infection BMSCs. The significance between the infection BMSCs and control BMSCs for uptake of 131I-FIAU at all the time points was t=23.06–173.83 and P<0.05 (n=3). Recombinant adenovirus vector had high infection and low toxic for BMSCs

and effective uptake of 131I-FIAU. This suggests a suitable gene vector for tracing genetically modified stem cells.