Temperature effects on fibrosis growth of glucagon was investigated by atomic force microscope.The protein fibers exhibit different surface structures under various incubation temperatures.At low temperature(20°C)

a majority of fibers take on helix structure.At high temperature(50°C)

the bulk of fibers do not have helix structure and they become a little thinner and much longer than that at low temperature.At midst temperature(35°C)

the amount of the two types of fiber are almost the same

and the thinner fiber grow much faster than the winder one at 35°C.These indicate that the two types of fiber have a different structure.In addition

we found that once the structure is formed

each type fiber was stable at different temperatures.