CA<sub>125</sub>时间分辨荧光免疫分析

蔡刚明; 黄飚; 朱利国; 肖华龙; 谭成; 陶永辉; 金坚

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CA₁₂₅时间分辨荧光免疫分析

更新时间：2021-03-12

- CA₁₂₅时间分辨荧光免疫分析
- Time-resolved fluoroimmunoassay of CA₁₂₅
- 核技术 2001年第5期
- 作者机构：
  
  核医学国家重点实验室!江苏省原子医学研究所无锡,214063
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R730.4
- 纸质出版日期：2001-05-01
- 稿件说明：
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[1]蔡刚明,黄飚,朱利国,肖华龙,谭成,陶永辉,金坚.CA_(125)时间分辨荧光免疫分析[J].核技术,2001(05):385-388.

CAI Gangming HUANG Biao ZHU Liguo XIAO Hualong TAN Cheng TAO Yonghui JIN Jian. Time-resolved fluoroimmunoassay of CA₁₂₅[J]. Nuclear techniques, 2001, (5): 385-388.
[1]蔡刚明,黄飚,朱利国,肖华龙,谭成,陶永辉,金坚.CA_(125)时间分辨荧光免疫分析[J].核技术,2001(05):385-388. DOI：

CAI Gangming HUANG Biao ZHU Liguo XIAO Hualong TAN Cheng TAO Yonghui JIN Jian. Time-resolved fluoroimmunoassay of CA₁₂₅[J]. Nuclear techniques, 2001, (5): 385-388. DOI：

摘要

以CA12 5单克隆抗体固相包被

用平衡饱和法与CA12 5、Eu3+标异硫氰酸苄基二乙烯三胺四乙酸 -CA12 5单抗形成三层夹心

以 β -二酮体为增强液

建立CA12 5的时间分辨荧光免疫分析法。数据采用Logit-Log函数和四参数Logistic函数数据处理程序处理。方法的批内和批间CV分别为 4 .5%和 4 .0 %

平均回收率 96.7%

灵敏度 3.3μg L

可测范围为 16— 2 0 62 μg L。本法与CEA无交叉

与AFP有 4 .6%交叉

与 β -HCG有 12 .4 %交叉。测定结果与进口IRMA试剂盒相比较

Abstract

A two-site time-resolved fluoroimmunoassay (TRFIA) of CA 125 based on the direct sandwich technique has been developed

with the equilibrium method. The monoclonal antibody (MoAb) against CA 125 was labelled with europium by the help of europium-chelate of diethylenetriaminepentaacetic acid (DTPA). The luminescent enhancement system was an enhancement solution that contained mainly of 2-naphthoyltrifluoroacetone. The intra- and inter- assay CV of the TRFIA were 4.5% and 4.0%

respectively

and the recovery rate was 96.7%

the sensitivity was 3.3μg/mL. The cross-reacting rate with CEA was negligible

and that with AFP and β-HCG was 4.6% and 12.4%

respectively. Compared with the imported IRMA Kit

the correlation coefficient was 0.999.

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CA125时间分辨荧光免疫分析

Time-resolved fluoroimmunoassay of CA125

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Abstract

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CA₁₂₅时间分辨荧光免疫分析

Time-resolved fluoroimmunoassay of CA₁₂₅