The fully automated synthesis of tumor imaging agents 18F-fluoroacetate （18F-FAC） and 1-H-1-（3-[18F]fluoro-2-hydro-xylpropyl）-2-nitroimidazole （18F-FMISO） via "one-pot" procedure in TRACERlab FXF-N synthesizer is described. 18F-FAC injection was prepared via two-step reactions including nucleophilic fluorination of the precursor benzyl bromoacetate with 18F-fluoride and subsequently hydrolysis with NaOH at the same reaction vessel

and purified with Sep Pak cartridges. The uncorrected radiochemical yield of 18F-FAC was about 45%

the radiochemical purity was more than 99%

and the whole synthesis time was less than 40 min. 18F-FMISO injection was prepared by the similar method. The uncorrected radiochemical yield of 18F-FMISO is above 40%

the radiochemical purity is above 95%

and the total synthesis time was less than 40 min. The fully automated one-pot synthesis procedures of 18F-FAC and 18F-FMISO injection are easy to operate and can be performed in any automated system designed for the synthesis of 2-18F-2-deoxy-D-glucose （18F-FDG） by the nucleophilic substitution.