To establish an IFMA of anti-HCV

the antigen of HCV was covered on the microwell plate

and then incubated with human anti-HCV in sample and Eu 3+ labeled goat anti-human IgG. The luminescent enhancement solution contains mainly 2-napthoyltrifluororacetate

and the data was treated with the log-logit function and four- parameter logistic function. Results showed that the intra- and inter- assay CV of the IFMA was 3.64% and 4.39%

respectively

and the recovery rate was 105.58%

the sensitivity was 0.03NCU/mL. The cross reactivity with HBsAg and HbeAg was 0.23% and 0.04%

respectively. The anti-HCV concentrations in serum of 278 health persons were all less than 1NCU/mL. Results indicated this new method was sensitive

stable and could be used as an effective non-radioassay tool for the basic or clinical study on HCV.