WANG Jufang HE Jing ZHOU Guangming LI Wenjian WEI Zengquan. DNA strand breakage of human hepatoma cells induced by γ-ray measured using the Comet Assay[J]. Nuclear techniques, 2001, (11): 895-898.
WANG Jufang HE Jing ZHOU Guangming LI Wenjian WEI Zengquan. DNA strand breakage of human hepatoma cells induced by γ-ray measured using the Comet Assay[J]. Nuclear techniques, 2001, (11): 895-898.DOI:
DNA strand breakage induced by γ-ray was studied using the Comet Assay. The human hepatoma cells were chosen as the test cell line. The results showed that the length of the tail appeared longer as the dose increased
and the number of cells with tail
increased with increasing dose. The length ratio and area ratio had positive correlation with the dose. Among them
the length ratio showed more obvious change with the increasing dose than area ratio
thus it could be used to describe the level of DNA strand breakage simply and efficiently.