After oral administration of perlolyrine and [2-15N] perlolyrine
urines of rats were hydrolyzed with glucuronidase
basified with NaHCO3-Na2CO3
and extracted with ethyl ether-iso-propyl alcohol etc. The organic phases (neutral and basic fractions) were concentrated for TMS derivatives. The aqueous phase were acidified with sulfuric acid
taken to dryness and extracted with methanol (water soluble acidic fractions) and concentrated for TMS derivatives. The TMS derivatives were determined by GC-MS. Perlolyrine and one metabolite were found from the neutral and basic fractions
and two different metabolites were found from the water soluble acidic fractions. It is proposed that the major metabolic pathways of perlolyrine are the hydroxylation of perlolyrine and the oxidition of its hydroxylmethyl group.