LIU Ning, JIN Jiannan, ZHANG Shuyuan, et al. 125I labeling of human serum albumins and their metabolic process in rabbits[J]. Nuclear techniques, 2000, (11): 816-820.
LIU Ning, JIN Jiannan, ZHANG Shuyuan, et al. 125I labeling of human serum albumins and their metabolic process in rabbits[J]. Nuclear techniques, 2000, (11): 816-820.DOI:
I labeling of three human serum albumins(HSAs) produced by different methods were performed by Chloramine-T
with yields of more than 80%. 125I labelled HSAs were purified by Sephadex G50 column with elution of 0.05mol/L PBS(pH7.4) and analysed by HPLC on a Sepheragel TSK phenyl SPW Column (7.5mm × 75mm)with gradient elution. The metabolic process of 125I-labelled HSAs in rabbits was investigated and their biological half lifes was calculated. The result showed that no significant difference were found among the biological half lifes of the three kinds of HSAs in rabbits.