The biological effects of nitrogen ion implantation (25 keV; 3.2×1016; 3.9×1016 and 4.6×1016 ions/cm2) on rape M1 generation were studied. The results showed that the germination percent of seeds treated at N+ doses between 3.2×1016 and 4.6×1016 ions/cm2 was not significantly different from control. It was observed that the emergent seedling and survival seedling percent of treated seeds at the dose of 3.9×1016 ions/cm2 was increased. Some agronomic and yield characters

such as plant height

number of leaves

fresh weight per plant at seedling stage

number of leaves

perimeter of stem

length and width of the biggest leaf at flower stage

number of primary branches

number of silique per plant and seed yield per plant at mature stage

were improved and increased at N+ treatment zone. According to the statistical result

the mutation frequency was about 3.2% in the field.