<sup>125</sup>I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究

史育红; 谭天秩; 项鹏

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¹²⁵I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究

更新时间：2021-03-12

- ¹²⁵I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究
- The characters of ¹²⁵I-VIP binding to the receptors derived from human gastrointestinal adenocarcinoma and their adjacent tissues
- 核技术 2000年第5期
- 作者机构：
  
  1. 华西医科大学第一临床医学院,成都,610041
  2. 华西医科大学基础医学院,成都,610041
  3. 华西医科大学第一临床医学院!成都610041华西医科大学基础医学院,成都,610041
- 作者简介：
- 基金信息：
  
  国家自然科学基金!39570225()
- DOI：
  中图分类号： R735
- 纸质出版日期：2000-05-01
- 稿件说明：
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[1]史育红,谭天秩,项鹏.~(125)I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究[J].核技术,2000(05):294-298.

The characters of ¹²⁵I-VIP binding to the receptors derived from human gastrointestinal adenocarcinoma and their adjacent tissues[J]. Nuclear techniques, 2000, (5): 294-298.
[1]史育红,谭天秩,项鹏.~(125)I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究[J].核技术,2000(05):294-298. DOI：

The characters of ¹²⁵I-VIP binding to the receptors derived from human gastrointestinal adenocarcinoma and their adjacent tissues[J]. Nuclear techniques, 2000, (5): 294-298. DOI：

摘要

探讨

１２５

Ｉ－血管活性肠肽（ＶＩＰ）与人胃腺癌及癌旁组织细胞膜、结肠腺癌及癌旁组织细胞膜的体外受体结合特性。

１２５

Ｉ－ＶＩＰ采用ＣＨ－Ｔ法制备，ＳｅｐｈａｄｅｘＧ－５０柱层析纯化，硅胶６０Ｆ

２５４

薄板层析（ＴＬＣ）检测。

１２５

－Ｉ－ＶＩＰ与人胃肠腺癌及癌旁组织细胞膜进行体外时间－温度结合实验、饱和结合实验和竞争结合实验。结果显示：碘标记率７０％，

１２５

Ｉ－ＶＩＰ比活度１８ＴＢｑ／ｍｍｏｌ，放射化学纯度９８％；

１２５

Ｉ－ＶＩＰ与人胃腺癌及癌旁组织细胞膜、结肠腺癌及癌旁组织细胞膜的结合具有温度与时间依赖性、可饱和性及特异性；Ｓｃａｔｃｈａｒｄ作图均为一直线，提示上述组织细胞膜含有单一位点ＶＩＰ受体；胃腺癌细胞膜及癌周组织细胞膜、结肠腺癌细胞膜及癌周组织细胞膜上ＶＩＰ受体的Ｋ

ｄ

值分别为１．９１、１．２６、０．９７、１．５３ｎｍｏｌ／Ｌ；Ｂ

ｍａｘ

分别为３３９、１５６、３０４、１５１ｆｍｏｌ／ｍｇ。研究结果表明人胃肠腺癌细胞膜与其癌旁组织细胞膜相比，ＶＩＰ受体亲和力无明显变化，最大结合容量明显增加，为ＶＩＰ受体显像奠定了直接的实验依据。

Abstract

To investigate the characteristics of

125

I-VIP binding to human gastrointestinal adenocarcinoma and their adjacent tissue. VIP was labeled with Na

125

I using chloramine -T method

separated by Sephadex G-50 column chromatography and examined by silica 60F

254

thin layer chromatography. The effects of time and temperature on the specific binding were examined

saturable binding and competitive binding between

125

I-VIP and VIP receptors in membranes from gastrointestinal adenocarcinoma and their adjacent tissue were carried out. The results showed that labeling rate was 70%

the specific activity of

125

I-VIP was 18TBq/mmol

the radiochemical purity was better than 98%. A single specific binding sites of high affinity were present in gastric adenocarcinoma and its adjacent tissue as well as in colon adenocarcinoma and its adjacent tissue. The K

and B

max

values were 1.19nmol/L and 339fmol/mg protein

1.26nmol/L and 156fmol/mg protein

0.97nmol/L and 304fmol/mg protein

1.53nmol/L and 151fmoll/mg protein respectively. The present study demonstrated that the binding sites of VIP on human gastrointestinal adenocarcinoma were more abundant than their adjacent tissue membrane

which provide valuable experimental evidence for VIP receptor imaging.

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125I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究

The characters of 125I-VIP binding to the receptors derived from human gastrointestinal adenocarcinoma and their adjacent tissues

DOI：

摘要

Abstract

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Keywords

references

¹²⁵I-VIP与人胃肠腺癌及癌旁组织细胞膜的体外受体结合特性研究

The characters of ¹²⁵I-VIP binding to the receptors derived from human gastrointestinal adenocarcinoma and their adjacent tissues