Graphene

a novel two-dimensional nanomaterial of sp2-bonded carbon atoms

has interested researchers in physics

chemistry and materials

because of its excellent electronic

optical and mechanical properties.And biological applications of graphene and its derivatives have been in rapid growth.The antibacterial activity of graphene oxide is always a hot topic.In this paper

we study antibacterial activity of graphene oxide(GO) by inhibition zone assay

turbidity estimation by spectrophotometer and plate count method

respectively.The results show that just GO exhibits antimicrobial activity via just the plate count method

with an inhibitory rate of about 80%.Owing to the physicochemical properties of GO

we believe that the plate count method can be used for detecting antimicrobial activity of GO.