XU Qiang1 YANG Tiqiang2, 3 WANG Miao4 1. Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase[J]. Nuclear techniques, 2011, 34(2): 147-150.
XU Qiang1 YANG Tiqiang2, 3 WANG Miao4 1. Effect of low energy N~+ ion beam implantation on the secondary structure and activity of α-amylase[J]. Nuclear techniques, 2011, 34(2): 147-150.DOI:
amples of the α-amylase were treated with 1015–1016 ions/cm2 of 20 keV N+.The secondary structure of the ion-implanted α-amylase was studied by circular dichroism
and activity change of the α-amylase was analyzed.The results showed that in the dose range under investigation
the N+ implantation affected the relative contents of α-helix
β-sheet
β-turn and random coil of the α-amylase
but no significant differences were found between the samples implanted to different doses.The activity of the N+-implanted α-amylase changed
with a relationship between the activity increase and the conformation change.