We transformed the transmission optical system of soft X-ray microscopy beam line

and established a platform suitable for studies on soft X-ray radiation damages to single cell.The X-ray energy of OK absorption edge was selected to scan Hela cells single by single.Single cell gel electrophoresis was used to evaluate the irradiation-induced damage effects.The results show that the radiation damage effect has dependence to the radiation dose.Thus

we provide a useful exploration for further works on radiation damage to single cells by soft X-rays.