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1.苏州医学院附属第一医院
2.苏州市传染病结核病医院(现改为苏州市第五人民医院)
收稿日期:1986-10-21,
纸质出版日期:1987-12
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杨永青, 江一民, 陆文栋, 等. 结核杆菌放射性快速药敏试验方法学的探讨及临床试验结果[J]. 核技术, 1987,10(12):50-53,62.
Yongqing Yang, Yimin Jiang, Wendong Lu, et al. Studies on mycobacterium tuberculosis sensitivity test by using the method of rapid radiometry with appendixes of clinical results[J]. Nuclear techniques, 1987, 10(12): 50-53,62.
杨永青, 江一民, 陆文栋, 等. 结核杆菌放射性快速药敏试验方法学的探讨及临床试验结果[J]. 核技术, 1987,10(12):50-53,62. DOI:
Yongqing Yang, Yimin Jiang, Wendong Lu, et al. Studies on mycobacterium tuberculosis sensitivity test by using the method of rapid radiometry with appendixes of clinical results[J]. Nuclear techniques, 1987, 10(12): 50-53,62. DOI:
目前,一般常规应用的结核杆菌药敏试验(例如改良罗氏法和琼脂稀释法)均需时较长,故有必要寻找一个快速药敏试验以供临床之需。结核杆菌快速药敏试验是需时较短,效果亦好的一种药敏试验方法,它系利用结核杆菌生长代谢时,能使
14
C-乙酸钠(CH
3
14
C O O Na)释放出
14
CO
2
的原理,测定
14
Co
2
的产量即可测知细菌的生长情况。为此,我们进行了方法学的探讨。
Three standard strains of mycobacterium tuberculosis (H
37
RV-fully sensitive
SM-R1000
μ
g/ml
RFP-R 100
μ
g/ml) were tested with 10 concentration of 5 antitubercular agent
INH
SM
PAS
RFP and EB. 114 isolates of mycobacterium tuberculosis taken from patients were tested with INH
PAS
SM and RFP
they were agreed with the results of standard Lowenstein-Jensen method is 81.7%. 82% of the isolate test were completed within 5 days. Our method may be used in routine clinical work. The liquid media that we prepared do not require human serum albumin and it is less expensive and readily available.
J. A. Kertcher et al ., Amer. Rev. Respir Dis. , 117 , 631 ( 1978 ).
郑翼宗 等 , 中华结核和呼吸系疾病杂志 , 3 , 21 ( 1980 )。
D. E. Snider et al ., Amer. Rev. Respir Dis . , 123 , 402 ( 1981 ).
郭润志 等 , 中华结核和呼吸系疾病杂志 , 7 , 339 ( 1984 )。
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