鸡烟碱样胆碱能受体与〔<sup>125</sup>I〕-<italic style="font-style: italic">α</italic>-银环蛇毒素结合动力学的研究

陈世铭

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鸡烟碱样胆碱能受体与〔¹²⁵I〕-α-银环蛇毒素结合动力学的研究

核技术在医学中的应用 | 更新时间：2022-09-13

- 鸡烟碱样胆碱能受体与〔¹²⁵I〕-α-银环蛇毒素结合动力学的研究
- Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick
- 核技术 1987年10卷第9期
- 作者机构：
  
  军事医学科学院药理毒理研究所
- 作者简介：
  
  *现在解放军总医院工作
- 基金信息：
- DOI：
  中图分类号：
- 收稿日期：1985-07-05，
  
  纸质出版日期：1987-09
- 稿件说明：
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陈世铭. 鸡烟碱样胆碱能受体与〔¹²⁵I〕-α-银环蛇毒素结合动力学的研究[J]. 核技术, 1987,10(9):53-56,44,65.

Shiming Chen. Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick[J]. Nuclear techniques, 1987, 10(9): 53-56,44,65.
陈世铭. 鸡烟碱样胆碱能受体与〔¹²⁵I〕-α-银环蛇毒素结合动力学的研究[J]. 核技术, 1987,10(9):53-56,44,65. DOI：

Shiming Chen. Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick[J]. Nuclear techniques, 1987, 10(9): 53-56,44,65. DOI：

摘要

从鸡脑及骨骼肌中分离提取烟碱样胆碱能受体，用[

125

I]-

-银环蛇毒素进行受体-配体结合动力学研究。结果表明，这种结合呈二级动力学反应性质。脑视叶中提纯的受体和骨骼肌中分离的受体，

值分别为129pM及4pM（25℃）。烟碱、箭毒碱等可抑制受体－配体的结合反应并测得各自的抑制常数及Hill系数。

Abstract

N-AChRs of chicken brain (either from optic lobe or from the rest of brain) and skeletal muscle were extracted in presence of protease inhibitors. The receptor of brain was further purified to homogeneity (pI=5.4) by affinity chromatography. The kinetic properties for binding of [

125

I]-

-BTX to detergent-solubilized and purified receptors from optie lobe and detergent-solubilized receptor from skeletal muscle were studied. It was confirmed that they are nicotinic. The binding of

-BTX to AChR from optic lobe was consistent with a simple bimolecular reaction. In contrast

it was biphasic to the muscle AChR. Dissociation constants (K

) for AChR-[

125

I]-

-BTX complex from optic lobe and from muscle were 129 pM and 4 pM respectively at 25℃. The reaction of [

125

I]-α-BXT with AChR was inhibited by agonists or antagonists and the

and h values were determined by Hill plot analysis of the data.

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references

陈世铭，国外医学分子生物学分册 , 6 , 2 , 56 ( 1984 )。

Z. Vogel et al. , Proc. Nat. Acad. Sci. USA. , 69 , 3180 ( 1972 ).

J. O. Dolly et al. , Biochemistry , 16 , 5053 ( 1977 ).

M. M. S. Lo et al. , Eur. J. Biochem. , 116 , 155 ( 1981 ).

U. Quast et al. , Biochemistry , 17 , 2405 ( 1978 ).

T. Heidmann et al. , Eur. J. Biochem. , 94 , 255 ( 1979 ).

B. J. Morley ， Pharmac. Ther. , 15 , 111 ( 1981 ).

G-K. Wang et al. , J. Biol. Chem. , 253 , 8507 ( 1978 ).

U. Z. Littauer et al. , Neurotransmitters and their receptors , John Wiley and Sons Ltd. , New york , 1980 , p. 271 .

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鸡烟碱样胆碱能受体与〔125I〕-α-银环蛇毒素结合动力学的研究

Studies on kinetic properties for binding of [125I]-α-bungarotoxin to nicotinic ACh receptors from chick

DOI：

摘要

Abstract

关键词

Keywords

references

鸡烟碱样胆碱能受体与〔¹²⁵I〕-α-银环蛇毒素结合动力学的研究

Studies on kinetic properties for binding of [¹²⁵I]-α-bungarotoxin to nicotinic ACh receptors from chick