Demin Xu, Chengzhang Zhu. Application of autoradiography and liquid scintillation counting in the study of proliferation of cultured cells of smooth muscle[J]. Nuclear techniques, 1984, 07(5): 66-67,53,77.
Demin Xu, Chengzhang Zhu. Application of autoradiography and liquid scintillation counting in the study of proliferation of cultured cells of smooth muscle[J]. Nuclear techniques, 1984, 07(5): 66-67,53,77.DOI:
The proliferative effect of hyperlipemic serum with or without Chinese herb medicine pollen typhae was compared in vitro.
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H-TdR was incorporated to DNA of cultured cells of smooth muscle of rabbit aorta. Radioactivity of DNA was determined by liquid scintillation counting (cpm) and the percentage of labelled nuclei was determined by autoradiography. It was found that the counting and the percentage of labelled nuclei were low in the cultured smooth muscle cells when hyperlipemic sera were added with pollen typhae than hyperlipemic sera were added alone. The results demonstated that pollen typhae has inhibiting effect on the stimulating action of hyperlipemic serum upon cells of smooth muscle.
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references
W. H. Hauss et al ., Atherosclerosis , 34 , 119 ( 1979 ).