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中国农业科学院原子能利用研究所
收稿日期:1984-03-16,
纸质出版日期:1984-10
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马昌燐, 温贤芳, 姚允寅, 等. 利用同位素15N对豆科作物固氮量测定的研究[J]. 核技术, 1984,07(5):47-50,74,75.
Changlin Ma, Xianfang Wen, Yunyin Yao, et al. Determination of nitrogen fixation of legume crops using isotope 15N[J]. Nuclear techniques, 1984, 07(5): 47-50,74,75.
本文报导了利用同位素
15
N对豆科作物固氮量测定的研究。结果表明,
15
N-
A
值法和总氮差值法测定五个大豆品种和两种豆科牧草的固氮量值显著相关,两方法的测定结果明显地呈线性关系。通过比较非固氮作物重复间总氮测量值和
15
N%原子超测定值变异系数的大小,表明用
A
N
值法估计固氮百分率比用总氮差值法可靠,试验结果还表明,
A
N
值法测定固氮量比总氮差值法和乙炔还原法具有一定的优越性。
Method for determination of N
2
fixed by legume crops is reported. By difference method and
15
N-A value method the amount of total N
2
fixed and percentages of total N
2
fixed by 5 soybeans and 2 species of legume grasses are highly corrected (r=0.96
y=1.051x+0.037 for the amount of N
2
fixation and r=0.39
y=0.245x+43.597 for the percentage of N
2
fixation). It is shown that the estimated percentage of N
2
fixed by A
N
-value method is more reliable than by difference method as a result of comparing the variation coefficents of total amount of N with the variation coefficents of
15
N% atom excess for non-fixing N
2
crops. It is found that the A
N
-value method has more advantageous than the difference method and the acetylene-ethylene method for measurement of dinitrogen fixation.
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R. Knowles et al ., Current perspectives in nitrogen fixation , 1981 , p. 327, 337. 482 .
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