
浏览全部资源
扫码关注微信
1.中国科学院上海生物化学研究所
2.复旦大学
收稿日期:1980-09-01,
纸质出版日期:1982-06
移动端阅览
陈法贤, 包俊茹, 邵清. 双标记样品液体闪烁测量法及使用电子计算机进行数据处理[J]. 核技术, 1982,05(3):6-11.
Faxian Chen, Junru Bao, Qing Shao. A method for liquid scintillation counting of dual labelled samples and data handling with a desk-top computer[J]. Nuclear techniques, 1982, 05(3): 6-11.
本文报导了
3
H和
32
P、
3
H和
14
C双标记样品液体闪烁测量法,使用仪器为Beckman LS8100和NE 8312液体闪烁谱仪。由双标记样品在仪器不同计数道中计数效率与
H
-数或外道比值
R
的关系,推导出计算双标记样品dpm的公式,所得数据用Wang 600型台式电子计算机处理。结果证明:这种测量和计算方法适用于双标记样品,并能得到满意的结果。
This paper described a method in which
3
H and
32
P
3
H and
14
C dual labelled samples were counted by a Beckman LS 8100 or an NE 8312 liquid scintillation spectrometer. An equation for calculating dpm of dual labelled samples was derived from the relation between the counting efficiency of dual labelled samples in different counting channels of a spectrometer and the H-number or external channel ratio value R. The data were processed by a Wang 600 calculator. The result shows that a satisfactory result can be obtained by using this method for measurement and calculation of dual labelled samples.
R. DE Wachter and W. Fiers , Anal. Biochem. , 18 , 2 , 351 ( 1967 ).
T. C. Hall and C. J. Weiser , Anal. Biochem. , 17 , 294 ( 1966 ).
曹梅讯 等 , 核技术 , 1 , 64 ( 1978 ).
E. J. C. Curtis and I. P. Toms , Liq. Scintill Counting , 2 , 167 ( 1971 ).
A. Dyer , An introduction to liq. scintill. counting , Heyden , London , 1974 , p. 69 .
A. G. Bruce and Olk C. Uhlenbeck , Nucleic Acids Research , 5 , 10 , 3665 ( 1978 ).
Donald L. Horrocks , Liq. Scintill. Counting , 5 , 145 ( 1977 ).
0
浏览量
28
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010802024621