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Shanghai Institute of Cell Biology, Academia Sinica
*现在上海昆虫研究所工作.
收稿日期:1979-06-18,
纸质出版日期:1981-02
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Cao Mei-xun, Liang Su-xiang, Dong Xiao-qin. Radioimmunoassay of progesterone[J]. Nuclear Techniques, 1981,04(1):11-15,29,29.
梅讯 曹, 素香 梁, 晓琴 董. 孕酮的放射免疫测定法[J]. 核技术, 1981, 04(1): 11-15,29,29.
本方法以
3
H-孕酮为标记抗原,孕酮-11α-琥珀酰—牛血清白蛋白为免疫原,能有效地测定生理水平的血清孕酮。本文对方法的专一性、灵敏度和精确度作了鉴定,并测定了正常妇女月经周期和中期怀孕妇女药物引产前后血清孕酮量的变化。
An efficient radioimmunoassay for determination of the physiological level of progesterone in human serum was developed
using
3
H-labelled progesterone as radiologand and progesterone-11α-succinyl-BSA as immunogen. It was applied to determine the serum progesterone level of women in regular menstruation in second trimester of pregnancy and administration of the aborfifacient.
I. H. Thorneycroft and S. C. Stone , Contraception , 5 , 129 ( 1972 ).
E. M. D. Cameron and J. J. Scarisbrick , Clin. Chem. , 19 , 1405 ( 1973 ).
A. Riondel et al ., J. Clin. Endocr. , 25 , 229 ( 1965 ).
H. J. vander Molen and D. Groen , J. Clin. Endocr. , 25 , 1625 ( 1965 ).
J. D. Neill et al ., J. Clin. Endocr. , 27 , 1167 ( 1967 ).
T. Yoshimi and M. B. Lipsett , Steroids , 11 , 528 ( 1968 ).
S. Furuyama and C. A. Nugent , Steroids , 17 , 663 ( 1971 ).
G. E. Abraham et al ., J. Clin. Endocr. , 32 , 619 ( 1971 ).
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